MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 6601–6700 (out of 7566)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
6432 886.9030 1771.7915
6434 886.9030 1771.7915
6435 886.9030 1771.7915
6436 886.9030 1771.7915
6437 886.9030 1771.7915
6439 900.4092 1798.8038
6440 900.4092 1798.8038
6441 900.4092 1798.8038
6442 900.4092 1798.8038
6443 900.4092 1798.8038
6444 900.4092 1798.8038
6445 900.4092 1798.8038
6446 900.4092 1798.8038
6447 900.4092 1798.8038
6448 900.4092 1798.8038
6450 900.4092 1798.8038
6451 900.4092 1798.8038
6452 900.4092 1798.8038
6453 900.4092 1798.8038
6454 900.4092 1798.8038
6456 900.4092 1798.8038
6457 900.4092 1798.8038
6458 900.4092 1798.8038
6459 900.4092 1798.8038
6460 900.4092 1798.8038
6461 900.4092 1798.8038
6463 900.4092 1798.8038
6464 900.4092 1798.8038
6465 900.4092 1798.8038
6466 900.4092 1798.8038
6467 900.4092 1798.8038
6468 900.4092 1798.8038
6469 900.4092 1798.8038
6470 900.4092 1798.8038
6471 900.4092 1798.8038
6472 900.4092 1798.8038
6474 900.4092 1798.8038
6475 900.4092 1798.8038
6476 900.4092 1798.8038
6480 900.4092 1798.8038
6481 900.4092 1798.8038
6484 900.4092 1798.8038
6486 900.4092 1798.8038
6487 900.4092 1798.8038
6490 900.4092 1798.8038
6491 900.4092 1798.8038
6492 900.4092 1798.8038
6493 900.4092 1798.8038
6494 900.4092 1798.8038
6495 900.4092 1798.8038
6498 900.4092 1798.8038
6499 900.4092 1798.8038
6503 900.4092 1798.8038
6506 900.4092 1798.8038
6508 900.4092 1798.8038
6510 900.4092 1798.8038
6513 900.4092 1798.8038
6514 900.4092 1798.8038
6515 900.4092 1798.8038
6516 900.4092 1798.8038
6517 900.4092 1798.8038
6518 900.4092 1798.8038
6519 900.4092 1798.8038
6520 900.4092 1798.8038
6522 900.4092 1798.8038
6525 900.4092 1798.8038
6526 900.4092 1798.8038
6527 900.4092 1798.8038
6529 900.4092 1798.8038
6531 900.4092 1798.8038
6532 900.4092 1798.8038
6533 900.4092 1798.8038
6534 900.4092 1798.8038
6536 900.4092 1798.8038
6537 900.4092 1798.8038
6538 900.4092 1798.8038
6540 900.4092 1798.8038
6541 900.4092 1798.8038
6542 900.4092 1798.8038
6543 900.4092 1798.8038
6544 900.4092 1798.8038
6545 900.4092 1798.8038
6546 900.4092 1798.8038
6547 900.4092 1798.8038
6548 900.4092 1798.8038
6549 900.4092 1798.8038
6550 900.4092 1798.8038
6551 900.4092 1798.8038
6552 900.4092 1798.8038
6553 900.4092 1798.8038
6555 900.4092 1798.8038
6557 900.4092 1798.8038
6558 900.4092 1798.8038
6559 900.4092 1798.8038
6560 900.4092 1798.8038
6562 900.4092 1798.8038
6564 900.4092 1798.8038
6565 900.4092 1798.8038
6567 900.4092 1798.8038
6568 900.4092 1798.8038
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