MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 6301–6400 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
6068 859.8907 1717.7669
6069 859.8907 1717.7669
6070 859.8907 1717.7669
6071 859.8907 1717.7669
6072 859.8907 1717.7669
6073 859.8907 1717.7669
6074 859.8907 1717.7669
6075 859.8907 1717.7669
6076 859.8907 1717.7669
6077 859.8907 1717.7669
6078 859.8907 1717.7669
6079 859.8907 1717.7669
6082 859.8907 1717.7669
6083 859.8907 1717.7669
6084 859.8907 1717.7669
6085 859.8907 1717.7669
6086 859.8907 1717.7669
6087 859.8907 1717.7669
6088 859.8907 1717.7669
6089 859.8907 1717.7669
6090 859.8907 1717.7669
6091 859.8907 1717.7669
6092 859.8907 1717.7669
6093 859.8907 1717.7669
6094 859.8907 1717.7669
6095 859.8907 1717.7669
6096 859.8907 1717.7669
6097 859.8907 1717.7669
6098 859.8907 1717.7669
6099 859.8907 1717.7669
6100 859.8907 1717.7669
6101 873.3969 1744.7793
6102 873.3969 1744.7793
6103 873.3969 1744.7793
6104 873.3969 1744.7793
6105 873.3969 1744.7793
6106 873.3969 1744.7793
6107 873.3969 1744.7793
6108 873.3969 1744.7793
6109 873.3969 1744.7793
6110 873.3969 1744.7793
6112 873.3969 1744.7793
6113 873.3969 1744.7793
6114 873.3969 1744.7793
6115 873.3969 1744.7793
6116 873.3969 1744.7793
6117 873.3969 1744.7793
6119 873.3969 1744.7793
6120 873.3969 1744.7793
6121 873.3969 1744.7793
6122 873.3969 1744.7793
6125 873.3969 1744.7793
6126 873.3969 1744.7793
6128 873.3969 1744.7793
6129 873.3969 1744.7793
6131 873.3969 1744.7793
6132 873.3969 1744.7793
6134 873.3969 1744.7793
6135 873.3969 1744.7793
6136 873.3969 1744.7793
6137 873.3969 1744.7793
6138 873.3969 1744.7793
6139 873.3969 1744.7793
6140 873.3969 1744.7793
6141 873.3969 1744.7793
6142 873.3969 1744.7793
6143 873.3969 1744.7793
6147 873.3969 1744.7793
6154 873.3969 1744.7793
6155 873.3969 1744.7793
6156 873.3969 1744.7793
6162 873.3969 1744.7793
6164 873.3969 1744.7793
6169 873.3969 1744.7793
6170 873.3969 1744.7793
6171 873.3969 1744.7793
6172 873.3969 1744.7793
6174 873.3969 1744.7793
6175 873.3969 1744.7793
6176 873.3969 1744.7793
6180 873.3969 1744.7793
6181 873.3969 1744.7793
6182 873.3969 1744.7793
6183 873.3969 1744.7793
6185 873.3969 1744.7793
6186 873.3969 1744.7793
6187 873.3969 1744.7793
6188 873.3969 1744.7793
6189 873.3969 1744.7793
6191 873.3969 1744.7793
6192 873.3969 1744.7793
6194 873.3969 1744.7793
6195 873.3969 1744.7793
6196 873.3969 1744.7793
6197 873.3969 1744.7793
6198 873.3969 1744.7793
6199 873.3969 1744.7793
6200 873.3969 1744.7793
6201 873.3969 1744.7793
6202 873.3969 1744.7793
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