MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 6201–6300 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
5953 859.8907 1717.7669
5954 859.8907 1717.7669
5956 859.8907 1717.7669
5957 859.8907 1717.7669
5958 859.8907 1717.7669
5959 859.8907 1717.7669
5960 859.8907 1717.7669
5962 859.8907 1717.7669
5963 859.8907 1717.7669
5965 859.8907 1717.7669
5968 859.8907 1717.7669
5969 859.8907 1717.7669
5970 859.8907 1717.7669
5972 859.8907 1717.7669
5973 859.8907 1717.7669
5974 859.8907 1717.7669
5975 859.8907 1717.7669
5976 859.8907 1717.7669
5978 859.8907 1717.7669
5979 859.8907 1717.7669
5980 859.8907 1717.7669
5982 859.8907 1717.7669
5983 859.8907 1717.7669
5984 859.8907 1717.7669
5986 859.8907 1717.7669
5987 859.8907 1717.7669
5988 859.8907 1717.7669
5989 859.8907 1717.7669
5990 859.8907 1717.7669
5991 859.8907 1717.7669
5992 859.8907 1717.7669
5993 859.8907 1717.7669
5994 859.8907 1717.7669
5995 859.8907 1717.7669
5996 859.8907 1717.7669
5997 859.8907 1717.7669
5998 859.8907 1717.7669
5999 859.8907 1717.7669
6000 859.8907 1717.7669
6001 859.8907 1717.7669
6003 859.8907 1717.7669
6004 859.8907 1717.7669
6005 859.8907 1717.7669
6006 859.8907 1717.7669
6007 859.8907 1717.7669
6008 859.8907 1717.7669
6009 859.8907 1717.7669
6010 859.8907 1717.7669
6011 859.8907 1717.7669
6012 859.8907 1717.7669
6013 859.8907 1717.7669
6014 859.8907 1717.7669
6015 859.8907 1717.7669
6016 859.8907 1717.7669
6017 859.8907 1717.7669
6018 859.8907 1717.7669
6019 859.8907 1717.7669
6020 859.8907 1717.7669
6021 859.8907 1717.7669
6022 859.8907 1717.7669
6023 859.8907 1717.7669
6024 859.8907 1717.7669
6025 859.8907 1717.7669
6026 859.8907 1717.7669
6027 859.8907 1717.7669
6028 859.8907 1717.7669
6029 859.8907 1717.7669
6030 859.8907 1717.7669
6031 859.8907 1717.7669
6032 859.8907 1717.7669
6033 859.8907 1717.7669
6034 859.8907 1717.7669
6036 859.8907 1717.7669
6037 859.8907 1717.7669
6038 859.8907 1717.7669
6039 859.8907 1717.7669
6041 859.8907 1717.7669
6043 859.8907 1717.7669
6044 859.8907 1717.7669
6045 859.8907 1717.7669
6046 859.8907 1717.7669
6047 859.8907 1717.7669
6048 859.8907 1717.7669
6049 859.8907 1717.7669
6050 859.8907 1717.7669
6051 859.8907 1717.7669
6052 859.8907 1717.7669
6053 859.8907 1717.7669
6054 859.8907 1717.7669
6055 859.8907 1717.7669
6056 859.8907 1717.7669
6059 859.8907 1717.7669
6060 859.8907 1717.7669
6061 859.8907 1717.7669
6062 859.8907 1717.7669
6063 859.8907 1717.7669
6064 859.8907 1717.7669
6065 859.8907 1717.7669
6066 859.8907 1717.7669
6067 859.8907 1717.7669
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