MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 6101–6200 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
5837 846.3846 1690.7546
5838 846.3846 1690.7546
5839 846.3846 1690.7546
5840 846.3846 1690.7546
5841 846.3846 1690.7546
5842 846.3846 1690.7546
5843 846.3846 1690.7546
5845 846.3846 1690.7546
5846 846.3846 1690.7546
5847 846.3846 1690.7546
5848 846.3846 1690.7546
5849 846.3846 1690.7546
5850 846.3846 1690.7546
5851 846.3846 1690.7546
5853 846.3846 1690.7546
5854 846.3846 1690.7546
5855 846.3846 1690.7546
5856 846.3846 1690.7546
5857 846.3846 1690.7546
5858 846.3846 1690.7546
5859 846.3846 1690.7546
5861 846.3846 1690.7546
5862 846.3846 1690.7546
5863 846.3846 1690.7546
5864 846.3846 1690.7546
5866 846.3846 1690.7546
5867 846.3846 1690.7546
5868 846.3846 1690.7546
5869 846.3846 1690.7546
5870 846.3846 1690.7546
5871 846.3846 1690.7546
5872 846.3846 1690.7546
5873 846.3846 1690.7546
5877 846.3846 1690.7546
5878 846.3846 1690.7546
5879 846.3846 1690.7546
5880 846.3846 1690.7546
5881 846.3846 1690.7546
5882 846.3846 1690.7546
5883 846.3846 1690.7546
5884 846.3846 1690.7546
5885 846.3846 1690.7546
5886 846.3846 1690.7546
5887 846.3846 1690.7546
5888 846.3846 1690.7546
5889 846.3846 1690.7546
5891 846.3846 1690.7546
5892 846.3846 1690.7546
5893 846.3846 1690.7546
5894 846.3846 1690.7546
5895 846.3846 1690.7546
5896 846.3846 1690.7546
5897 846.3846 1690.7546
5898 846.3846 1690.7546
5900 846.3846 1690.7546
5901 846.3846 1690.7546
5902 846.3846 1690.7546
5903 846.3846 1690.7546
5905 846.3846 1690.7546
5906 846.3846 1690.7546
5907 846.3846 1690.7546
5908 846.3846 1690.7546
5910 846.3846 1690.7546
5911 846.3846 1690.7546
5912 846.3846 1690.7546
5913 846.3846 1690.7546
5914 846.3846 1690.7546
5916 846.3846 1690.7546
5917 846.3846 1690.7546
5918 846.3846 1690.7546
5919 846.3846 1690.7546
5920 846.3846 1690.7546
5921 846.3846 1690.7546
5922 846.3846 1690.7546
5923 846.3846 1690.7546
5924 846.3846 1690.7546
5925 846.3846 1690.7546
5926 846.3846 1690.7546
5927 846.3846 1690.7546
5928 846.3846 1690.7546
5929 846.3846 1690.7546
5930 846.3846 1690.7546
5931 846.3846 1690.7546
5932 859.8907 1717.7669
5933 859.8907 1717.7669
5934 859.8907 1717.7669
5935 859.8907 1717.7669
5936 859.8907 1717.7669
5937 859.8907 1717.7669
5938 859.8907 1717.7669
5939 859.8907 1717.7669
5940 859.8907 1717.7669
5941 859.8907 1717.7669
5942 859.8907 1717.7669
5943 859.8907 1717.7669
5944 859.8907 1717.7669
5945 859.8907 1717.7669
5946 859.8907 1717.7669
5950 859.8907 1717.7669
5952 859.8907 1717.7669
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