MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 6001–6100 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
5691 832.8785 1663.7424
5696 832.8785 1663.7424
5697 832.8785 1663.7424
5699 832.8785 1663.7424
5703 832.8785 1663.7424
5705 832.8785 1663.7424
5706 832.8785 1663.7424
5707 832.8785 1663.7424
5708 832.8785 1663.7424
5709 832.8785 1663.7424
5710 832.8785 1663.7424
5711 832.8785 1663.7424
5713 832.8785 1663.7424
5714 832.8785 1663.7424
5715 832.8785 1663.7424
5716 832.8785 1663.7424
5717 832.8785 1663.7424
5718 832.8785 1663.7424
5719 832.8785 1663.7424
5720 832.8785 1663.7424
5721 832.8785 1663.7424
5722 832.8785 1663.7424
5723 832.8785 1663.7424
5724 832.8785 1663.7424
5726 832.8785 1663.7424
5727 832.8785 1663.7424
5728 832.8785 1663.7424
5729 832.8785 1663.7424
5730 832.8785 1663.7424
5731 832.8785 1663.7424
5732 832.8785 1663.7424
5733 832.8785 1663.7424
5734 832.8785 1663.7424
5736 832.8785 1663.7424
5737 832.8785 1663.7424
5738 832.8785 1663.7424
5739 832.8785 1663.7424
5740 832.8785 1663.7424
5741 832.8785 1663.7424
5742 832.8785 1663.7424
5743 832.8785 1663.7424
5745 832.8785 1663.7424
5746 832.8785 1663.7424
5747 832.8785 1663.7424
5748 832.8785 1663.7424
5749 832.8785 1663.7424
5750 832.8785 1663.7424
5751 832.8785 1663.7424
5752 832.8785 1663.7424
5753 832.8785 1663.7424
5754 832.8785 1663.7424
5755 832.8785 1663.7424
5756 832.8785 1663.7424
5757 832.8785 1663.7424
5758 832.8785 1663.7424
5760 832.8785 1663.7424
5761 832.8785 1663.7424
5762 832.8785 1663.7424
5763 846.3846 1690.7546
5764 846.3846 1690.7546
5765 846.3846 1690.7546
5766 846.3846 1690.7546
5768 846.3846 1690.7546
5769 846.3846 1690.7546
5770 846.3846 1690.7546
5771 846.3846 1690.7546
5772 846.3846 1690.7546
5773 846.3846 1690.7546
5774 846.3846 1690.7546
5775 846.3846 1690.7546
5776 846.3846 1690.7546
5777 846.3846 1690.7546
5778 846.3846 1690.7546
5779 846.3846 1690.7546
5780 846.3846 1690.7546
5781 846.3846 1690.7546
5788 846.3846 1690.7546
5789 846.3846 1690.7546
5790 846.3846 1690.7546
5791 846.3846 1690.7546
5793 846.3846 1690.7546
5794 846.3846 1690.7546
5795 846.3846 1690.7546
5797 846.3846 1690.7546
5804 846.3846 1690.7546
5808 846.3846 1690.7546
5809 846.3846 1690.7546
5811 846.3846 1690.7546
5815 846.3846 1690.7546
5816 846.3846 1690.7546
5817 846.3846 1690.7546
5818 846.3846 1690.7546
5822 846.3846 1690.7546
5824 846.3846 1690.7546
5826 846.3846 1690.7546
5828 846.3846 1690.7546
5830 846.3846 1690.7546
5832 846.3846 1690.7546
5833 846.3846 1690.7546
5834 846.3846 1690.7546
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