MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 5901–6000 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
5571 819.3723 1636.7301
5572 819.3723 1636.7301
5573 819.3723 1636.7301
5574 819.3723 1636.7301
5575 819.3723 1636.7301
5576 819.3723 1636.7301
5577 819.3723 1636.7301
5578 819.3723 1636.7301
5579 819.3723 1636.7301
5580 819.3723 1636.7301
5582 819.3723 1636.7301
5583 819.3723 1636.7301
5584 819.3723 1636.7301
5585 819.3723 1636.7301
5586 819.3723 1636.7301
5587 819.3723 1636.7301
5588 819.3723 1636.7301
5589 819.3723 1636.7301
5590 819.3723 1636.7301
5591 819.3723 1636.7301
5594 832.8785 1663.7424
5595 832.8785 1663.7424
5596 832.8785 1663.7424
5597 832.8785 1663.7424
5598 832.8785 1663.7424
5599 832.8785 1663.7424
5600 832.8785 1663.7424
5601 832.8785 1663.7424
5602 832.8785 1663.7424
5604 832.8785 1663.7424
5605 832.8785 1663.7424
5607 832.8785 1663.7424
5609 832.8785 1663.7424
5611 832.8785 1663.7424
5612 832.8785 1663.7424
5615 832.8785 1663.7424
5616 832.8785 1663.7424
5623 832.8785 1663.7424
5624 832.8785 1663.7424
5625 832.8785 1663.7424
5626 832.8785 1663.7424
5627 832.8785 1663.7424
5628 832.8785 1663.7424
5629 832.8785 1663.7424
5630 832.8785 1663.7424
5632 832.8785 1663.7424
5633 832.8785 1663.7424
5634 832.8785 1663.7424
5635 832.8785 1663.7424
5636 832.8785 1663.7424
5637 832.8785 1663.7424
5638 832.8785 1663.7424
5639 832.8785 1663.7424
5640 832.8785 1663.7424
5641 832.8785 1663.7424
5642 832.8785 1663.7424
5643 832.8785 1663.7424
5644 832.8785 1663.7424
5645 832.8785 1663.7424
5646 832.8785 1663.7424
5647 832.8785 1663.7424
5648 832.8785 1663.7424
5649 832.8785 1663.7424
5650 832.8785 1663.7424
5651 832.8785 1663.7424
5652 832.8785 1663.7424
5653 832.8785 1663.7424
5654 832.8785 1663.7424
5656 832.8785 1663.7424
5657 832.8785 1663.7424
5658 832.8785 1663.7424
5659 832.8785 1663.7424
5660 832.8785 1663.7424
5663 832.8785 1663.7424
5664 832.8785 1663.7424
5665 832.8785 1663.7424
5666 832.8785 1663.7424
5667 832.8785 1663.7424
5668 832.8785 1663.7424
5669 832.8785 1663.7424
5670 832.8785 1663.7424
5671 832.8785 1663.7424
5672 832.8785 1663.7424
5673 832.8785 1663.7424
5674 832.8785 1663.7424
5675 832.8785 1663.7424
5676 832.8785 1663.7424
5677 832.8785 1663.7424
5678 832.8785 1663.7424
5679 832.8785 1663.7424
5680 832.8785 1663.7424
5681 832.8785 1663.7424
5682 832.8785 1663.7424
5683 832.8785 1663.7424
5684 832.8785 1663.7424
5685 832.8785 1663.7424
5686 832.8785 1663.7424
5687 832.8785 1663.7424
5689 832.8785 1663.7424
5690 832.8785 1663.7424
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