MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 501–600 (out of 7566)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
5909 846.3846 3381.5092 3381.4772 9.47 1 5 1 +1Score > 20 indicates identity
Score > 17 indicates homology
NFKVPFNFYNVHYNSTHVVGTSGGSTDDMK + 4 Deamidated (NQ); Oxidation (M)
2098 549.2496 1096.4846 1096.4753 8.44 1 4 1 +1Score > 20 indicates identity
Score > 17 indicates homology
GDARMMASSR + Oxidation (M)
5786 846.3846 2536.1319 2536.1322 -0.10 0 4 1 +1Score > 20 indicates identity
Score > 17 indicates homology
AASMITESQCYQLEQNLHQQR + 2 Deamidated (NQ)
6554 900.4092 3597.6076 3597.5529 15.2 1 4 1 +1Score > 20 indicates identity
Score > 17 indicates homology
VGCMLAMVPLYPYSCNPDDVMFAQESMRER + 2 Oxidation (M)
74 387.1759 1544.6744 1544.6889 -9.41 0 4 0.87 +1Score > 16 indicates identity QAGCTLHEVGTTNR + 2 Deamidated (NQ)
1143 468.2127 1868.8217 1868.8496 -14.9 1 4 1 +1Score > 20 indicates identity
Score > 17 indicates homology
MSKSIGNTVSPQDVMNK + 2 Deamidated (NQ); 2 Oxidation (M)
6854 927.4214 1852.8283 1852.8295 -0.61 1 4 1 +1Score > 21 indicates identity
Score > 17 indicates homology
QDIRAMQDQVMELSR + 2 Deamidated (NQ); 2 Oxidation (M)
480 414.1881 826.3617 826.3564 6.40 1 4 1.7 +1Score > 19 indicates identity MKMEEK + 2 Oxidation (M)
629 427.6943 853.3741 853.3600 16.5 0 4 0.77 +1Score > 16 indicates identity MTSSAGQR + Deamidated (NQ); Oxidation (M)
6489 900.4092 2698.2057 2698.1884 6.41 1 4 0.56 +1Score > 20 indicates identity
Score > 14 indicates homology
CVEADCGQMQEELDLTISAETRK + Oxidation (M)
156 387.1759 1544.6744 1544.6889 -9.41 0 4 0.88 +1Score > 16 indicates identity QAGCTLHEVGTTNR + 2 Deamidated (NQ)
6881 927.4214 1852.8283 1852.8625 -18.4 1 4 1 +1Score > 21 indicates identity
Score > 17 indicates homology
LNNKEMNAQLDYLNR + 2 Deamidated (NQ); Oxidation (M)
474 414.1881 826.3617 826.3531 10.5 0 4 1.7 +1Score > 19 indicates identity MFQQQK + 2 Deamidated (NQ); Oxidation (M)
3457 657.2986 1968.8741 1968.8591 7.62 0 4 0.75 +1Score > 21 indicates identity
Score > 16 indicates homology
MPNVDVVGEMVNTMSASR + Deamidated (NQ); 2 Oxidation (M)
5143 792.3600 2374.0583 2374.0899 -13.3 0 4 1 +1Score > 21 indicates identity
Score > 17 indicates homology
LSANWMAAAGHPGEDAGLYEAVK + Deamidated (NQ); Oxidation (M)
868 454.7066 1814.7972 1814.8100 -7.07 0 4 1.1 +1Score > 17 indicates identity VMQVSAMADQMLLDSK + Deamidated (NQ); 3 Oxidation (M)
6040 859.8907 3435.5339 3435.5929 -17.2 1 4 1.4 +1Score > 18 indicates identity RQMLNEHYDVTTAAYAVGYESYPISVGNIR + 3 Deamidated (NQ); Oxidation (M)
2178 549.2496 1096.4846 1096.4753 8.44 1 4 0.69 +1Score > 20 indicates identity
Score > 15 indicates homology
GDARMMASSR + Oxidation (M)
4449 738.3355 2211.9847 2212.0140 -13.2 1 4 0.39 +1Score > 20 indicates identity
Score > 13 indicates homology
MGRTQLQDAVPMTLGQEFR + 3 Deamidated (NQ); 2 Oxidation (M)
4447 738.3355 1474.6565 1474.6657 -6.24 1 4 1 +1Score > 20 indicates identity
Score > 17 indicates homology
ELHMGRMSQPTR + Deamidated (NQ); 2 Oxidation (M)
1680 508.7311 2030.8955 2030.8646 15.2 0 4 1 +1Score > 17 indicates identity EGYHQDWNTLIYNYGR + 3 Deamidated (NQ)
4470 738.3355 1474.6565 1474.6834 -18.3 1 4 1 +1Score > 20 indicates identity
Score > 17 indicates homology
RETQQMPEVGQR + Deamidated (NQ); Oxidation (M)
5480 819.3723 3273.4602 3273.5169 -17.3 0 4 1 +1Score > 21 indicates identity
Score > 17 indicates homology
MDAFIQYGIVAGVQAMQDSGLEITEENATR + Deamidated (NQ); Oxidation (M)
990 454.7066 1814.7972 1814.7781 10.5 1 4 1.2 +1Score > 17 indicates identity NMTFSKDDWQTQEGK + Deamidated (NQ)
4783 765.3478 3057.3620 3057.4162 -17.7 1 4 0.6 +1Score > 21 indicates identity
Score > 15 indicates homology
LEPQAEDAPLNNYANERLTQLNQQQR + 5 Deamidated (NQ)
1767 522.2372 2084.9199 2084.9296 -4.66 0 4 1.8 +1Score > 19 indicates identity EHVATGIDCYMQQFGVSK + Oxidation (M)
2765 603.2741 1806.8005 1806.7818 10.4 1 4 0.7 +1Score > 20 indicates identity
Score > 15 indicates homology
YRYAFINCTQCGPR + 2 Deamidated (NQ)
3805 684.3109 1366.6073 1366.6221 -10.8 0 4 1 +1Score > 20 indicates identity
Score > 17 indicates homology
LANAAITDMQMR + Deamidated (NQ); 2 Oxidation (M)
85 387.1759 1158.5058 1158.5267 -18.0 0 4 0.9 +1Score > 16 indicates identity EAYELFMEK
4534 738.3355 1474.6565 1474.6623 -3.95 0 4 1 +1Score > 20 indicates identity
Score > 17 indicates homology
TNNMTLHTNWAR + Deamidated (NQ); Oxidation (M)
250 400.6820 1598.6989 1598.7186 -12.3 1 4 0.83 +1Score > 16 indicates identity GGQHSGGNFKNDPQR + Deamidated (NQ)
5366 805.8662 1609.7179 1609.7195 -1.00 0 4 1.5 +1Score > 19 indicates identity HCFSVVAQNQQYK + 2 Deamidated (NQ)
5693 832.8785 3327.4848 3327.5071 -6.69 1 4 1.7 +1Score > 19 indicates identity NCATQFISCQDSVAALGMRVLGNPYGNDPR + Deamidated (NQ); Oxidation (M)
5961 859.8907 3435.5339 3435.5818 -14.0 0 4 1.4 +1Score > 18 indicates identity DVNALMTAGQLINWGMPTLAAEMLNALDCQR + 3 Deamidated (NQ); Oxidation (M)
1072 468.2127 1401.6163 1401.6194 -2.24 0 4 1 +1Score > 20 indicates identity
Score > 17 indicates homology
TLPPGCEGEEASR
48 387.1759 1158.5058 1158.5016 3.64 0 4 0.91 +1Score > 16 indicates identity CVQQFDFSK + Deamidated (NQ)
743 441.2004 1320.5795 1320.6054 -19.6 0 4 1 +1Score > 20 indicates identity
Score > 17 indicates homology
SQLPGMVEDAMK + Oxidation (M)
5785 846.3846 2536.1319 2536.1322 -0.10 0 4 1 +1Score > 20 indicates identity
Score > 17 indicates homology
AASMITESQCYQLEQNLHQQR + 2 Deamidated (NQ)
3467 657.2986 1968.8741 1968.8809 -3.43 0 4 0.61 +1Score > 21 indicates identity
Score > 15 indicates homology
YVLGDMLGQSPQMEQVR + 3 Deamidated (NQ); Oxidation (M)
3827 684.3109 2049.9109 2049.9174 -3.15 0 4 1 +1Score > 20 indicates identity
Score > 17 indicates homology
NAMQDLIDHAINHADNNK + Deamidated (NQ); Oxidation (M)
2183 549.2496 1096.4846 1096.4753 8.44 1 4 1 +1Score > 20 indicates identity
Score > 17 indicates homology
GDARMMASSR + Oxidation (M)
3254 643.7925 2571.1411 2571.1759 -13.5 0 4 1.5 +1Score > 18 indicates identity NNVTTDAGAGFEQTLTGMTVGIDSR + Deamidated (NQ); Oxidation (M)
1551 508.7311 2030.8955 2030.9037 -4.05 1 4 1 +1Score > 17 indicates identity HGNSEMQKINQTSAMPEK + 2 Deamidated (NQ)
3416 657.2986 1968.8741 1968.8809 -3.43 1 4 0.79 +1Score > 21 indicates identity
Score > 16 indicates homology
MVETSQCVPYPAKADEK + Deamidated (NQ); Oxidation (M)
91 387.1759 1544.6744 1544.6889 -9.41 0 4 0.93 +1Score > 16 indicates identity QAGCTLHEVGTTNR + 2 Deamidated (NQ)
2299 562.7557 1123.4968 1123.4968 0.031 1 4 1.4 +1Score > 18 indicates identity MREYPNGEK + Deamidated (NQ)
4106 711.3232 2130.9479 2130.9124 16.7 0 4 0.64 +1Score > 21 indicates identity
Score > 15 indicates homology
NNTVYSAATQTGVCQGDTSR + 2 Deamidated (NQ)
1795 522.2372 1563.6899 1563.6844 3.55 0 4 1.8 +1Score > 19 indicates identity MICSPQNNTGAPMK + Oxidation (M)
40 387.1759 772.3372 772.3351 2.67 0 4 0.93 +1Score > 16 indicates identity AQGEDPR + Deamidated (NQ)
167 387.1759 772.3372 772.3464 -11.9 0 4 0.93 +1Score > 16 indicates identity NNPDGTR
3323 643.7925 1285.5705 1285.5946 -18.7 1 4 1.5 +1Score > 18 indicates identity SGRIDMGHGAGGR + Oxidation (M)
783 441.2004 1760.7727 1760.8039 -17.8 0 4 1 +1Score > 20 indicates identity
Score > 17 indicates homology
MPDAEFQSHIQLDSK + Oxidation (M)
7158 954.4337 1906.8529 1906.8883 -18.6 0 4 0.51 +1Score > 21 indicates identity
Score > 14 indicates homology
WSSQMINTLQIQFHR + 3 Deamidated (NQ); Oxidation (M)
456 414.1881 826.3617 826.3565 6.38 0 4 1.8 +1Score > 19 indicates identity MLGQMSK + Deamidated (NQ); 2 Oxidation (M)
2119 549.2496 1096.4846 1096.5005 -14.5 1 4 0.61 +1Score > 20 indicates identity
Score > 15 indicates homology
KLDMNSMAR + 2 Oxidation (M)
836 441.2004 1320.5795 1320.6058 -19.9 1 4 1 +1Score > 20 indicates identity
Score > 17 indicates homology
DNNRVGFAEAAR + 2 Deamidated (NQ)
5450 819.3723 3273.4602 3273.4363 7.30 0 4 0.53 +1Score > 21 indicates identity
Score > 14 indicates homology
YNGLPSMEIQGEAAPGTSSGDAMALMENLASK + 2 Deamidated (NQ); 2 Oxidation (M)
931 454.7066 907.3986 907.3817 18.6 1 4 1.2 +1Score > 17 indicates identity RDNQAMR + 2 Deamidated (NQ); Oxidation (M)
3920 697.8171 1393.6196 1393.6031 11.9 0 4 1.8 +1Score > 19 indicates identity TEQVNQQLMER + 3 Deamidated (NQ); Oxidation (M)
1547 508.7311 1015.4477 1015.4611 -13.1 0 4 1.1 +1Score > 17 indicates identity YYDAETVR
1961 535.7434 1069.4723 1069.4862 -13.0 1 4 1.2 +1Score > 18 indicates identity DAYREMAAK + Oxidation (M)
5592 819.3723 2455.0951 2455.0696 10.4 0 4 0.49 +1Score > 21 indicates identity
Score > 14 indicates homology
STPDFSTAENNQELANEVSCLK + 2 Deamidated (NQ)
2035 535.7434 1069.4723 1069.4637 7.97 0 4 1.3 +1Score > 18 indicates identity IEQFMQQK + 3 Deamidated (NQ); Oxidation (M)
2102 549.2496 1096.4846 1096.4753 8.44 1 4 1 +1Score > 20 indicates identity
Score > 17 indicates homology
GDARMMASSR + Oxidation (M)
3616 670.8048 2679.1901 2679.2421 -19.4 1 4 1.8 +1Score > 19 indicates identity RAVNPAVMMNDTLFNALNDWVDR + 2 Deamidated (NQ); Oxidation (M)
4114 711.3232 2841.2639 2841.2491 5.19 0 4 0.55 +1Score > 21 indicates identity
Score > 14 indicates homology
THEGNRPGDNWQFDGDAETIAHFAR + Deamidated (NQ)
7370 967.9399 3867.7304 3867.7261 1.11 0 4 1.8 +1Score > 19 indicates identity EQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAK + 7 Deamidated (NQ)
721 441.2004 1760.7727 1760.7709 0.99 0 4 1 +1Score > 20 indicates identity
Score > 17 indicates homology
MMSEGLDPLAPADENR + Oxidation (M)
4115 711.3232 1420.6319 1420.6293 1.87 0 4 0.74 +1Score > 21 indicates identity
Score > 15 indicates homology
NLQTTGCESWPK + Deamidated (NQ)
6124 873.3969 1744.7793 1744.8002 -12.0 0 4 0.5 +1Score > 22 indicates identity
Score > 14 indicates homology
IADALINEAQQDLQSN + 3 Deamidated (NQ)
47 387.1759 1158.5058 1158.5267 -18.0 0 4 0.96 +1Score > 16 indicates identity EAYELFMEK
1850 522.2372 1563.6899 1563.7205 -19.6 0 4 1.9 +1Score > 19 indicates identity AADAAFAEWGQTTPK + Deamidated (NQ)
553 427.6943 853.3741 853.3851 -13.0 0 4 0.85 +1Score > 16 indicates identity GEMSTVSK + Oxidation (M)
403 414.1881 1239.5426 1239.5661 -19.0 0 4 1.9 +1Score > 19 indicates identity SPMELMTMLR + 2 Oxidation (M)
714 441.2004 880.3863 880.3709 17.5 0 4 0.58 +1Score > 20 indicates identity
Score > 14 indicates homology
ATDSATCR
1771 522.2372 1563.6899 1563.7205 -19.6 0 4 1.9 +1Score > 19 indicates identity FESTGSQLTFAGYR + Deamidated (NQ)
7371 967.9399 3867.7304 3867.7261 1.11 0 4 1.9 +1Score > 19 indicates identity EQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAK + 7 Deamidated (NQ)
428 414.1881 826.3617 826.3643 -3.13 0 4 1.9 +1Score > 19 indicates identity MYVNQR + Deamidated (NQ); Oxidation (M)
5270 805.8662 3219.4357 3219.4973 -19.1 1 4 1.6 +1Score > 19 indicates identity VAMMNRLQQALEHNHFFLMAQPITGMR + 4 Deamidated (NQ); 2 Oxidation (M)
58 387.1759 772.3372 772.3351 2.67 0 4 0.97 +1Score > 16 indicates identity AQGEDPR + Deamidated (NQ)
2445 576.2618 1150.5091 1150.5077 1.26 1 4 1 +1Score > 20 indicates identity
Score > 17 indicates homology
DTANMQRWK + 2 Deamidated (NQ)
3515 657.2986 1968.8741 1968.8734 0.34 0 4 1 +1Score > 21 indicates identity
Score > 16 indicates homology
DYSELNVAQGQEMIAQR + 2 Deamidated (NQ); Oxidation (M)
3649 670.8048 2679.1901 2679.2349 -16.7 0 4 1.9 +1Score > 19 indicates identity TVYNQPANMFVSGFIGSPAMNFIR + 3 Deamidated (NQ); Oxidation (M)
3979 697.8171 1393.6196 1393.6329 -9.58 1 4 1.9 +1Score > 19 indicates identity QAQQMQEKMQK + Deamidated (NQ); Oxidation (M)
5803 846.3846 3381.5092 3381.5291 -5.87 0 4 0.88 +1Score > 20 indicates identity
Score > 16 indicates homology
DGCIAIACPITGDWVAMTNHTWAFSIAEMK + Oxidation (M)
1854 522.2372 2084.9199 2084.9261 -3.00 1 4 1.9 +1Score > 19 indicates identity QYLNMQNGANFGPWRLR + 5 Deamidated (NQ); Oxidation (M)
3922 697.8171 2787.2392 2787.2520 -4.59 0 4 1.9 +1Score > 19 indicates identity AGIPLDVMYDVVTNAAGNSWMFENR + 2 Deamidated (NQ); Oxidation (M)
1483 495.2250 988.4354 988.4358 -0.40 0 4 1 +1Score > 20 indicates identity
Score > 16 indicates homology
ISSGGMMFK + 2 Oxidation (M)
7232 954.4337 2860.2793 2860.2466 11.4 1 4 1 +1Score > 21 indicates identity
Score > 16 indicates homology
YMGNNGPMQLEVPRDQYAGAVETMR + 2 Deamidated (NQ); 2 Oxidation (M)
1398 495.2250 1482.6531 1482.6521 0.67 1 4 1 +1Score > 20 indicates identity
Score > 16 indicates homology
MNNNDLFQASRR + 2 Deamidated (NQ); Oxidation (M)
1725 522.2372 2084.9199 2084.9287 -4.22 0 4 2 +1Score > 19 indicates identity QTSLNGFYQDNGGDADLLR + 2 Deamidated (NQ)
6598 900.4092 3597.6076 3597.6697 -17.3 1 4 0.65 +1Score > 20 indicates identity
Score > 15 indicates homology
VKSWWVNAGEAFMIYSLAENFCSSNGYTLPR + Deamidated (NQ)
1093 468.2127 1868.8217 1868.8363 -7.78 0 4 1 +1Score > 20 indicates identity
Score > 16 indicates homology
MIEHSGGAESLANYFSR + Deamidated (NQ)
1436 495.2250 1482.6531 1482.6806 -18.6 0 4 1 +1Score > 20 indicates identity
Score > 16 indicates homology
MVNSGTEATMSAIR + Oxidation (M)
3986 697.8171 1393.6196 1393.6473 -19.9 1 4 1.9 +1Score > 19 indicates identity NESAGEQLRFGGK + 2 Deamidated (NQ)
3413 657.2986 1312.5827 1312.5643 14.0 1 4 1 +1Score > 21 indicates identity
Score > 16 indicates homology
HDVKGDNEENR + Deamidated (NQ)
1086 468.2127 934.4109 934.4145 -3.85 0 4 0.6 +1Score > 20 indicates identity
Score > 14 indicates homology
TDGTWEAR
3044 616.7802 2463.0917 2463.0570 14.1 1 4 1.5 +1Score > 18 indicates identity LNKEMVEGMGGTQSEQYQEFR + 3 Deamidated (NQ)
2419 576.2618 1725.7637 1725.7879 -14.0 0 4 0.63 +1Score > 20 indicates identity
Score > 14 indicates homology
AELGNNTEYDMLALR + Deamidated (NQ); Oxidation (M)
6167 873.3969 3489.5585 3489.5468 3.36 1 4 1 +1Score > 22 indicates identity
Score > 16 indicates homology
GDGCGHCAACNLRANGLNHYLADKPTVMAAMK + Deamidated (NQ); Oxidation (M)
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