MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
Repeat search

[help]

Export

Not what you expected? Try the select summary.

Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

Filters

[help]

Show

Unassigned peptides, 5801–5900 (out of 7566)

Page: Previous 1 …54 55 56 57 58 59 60 61 62 63 64 … 76 Next 

Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
5422 805.8662 1609.7179
5424 805.8662 1609.7179
5425 819.3723 1636.7301
5426 819.3723 1636.7301
5427 819.3723 1636.7301
5428 819.3723 1636.7301
5429 819.3723 1636.7301
5430 819.3723 1636.7301
5431 819.3723 1636.7301
5432 819.3723 1636.7301
5433 819.3723 1636.7301
5434 819.3723 1636.7301
5435 819.3723 1636.7301
5436 819.3723 1636.7301
5437 819.3723 1636.7301
5438 819.3723 1636.7301
5439 819.3723 1636.7301
5440 819.3723 1636.7301
5441 819.3723 1636.7301
5443 819.3723 1636.7301
5444 819.3723 1636.7301
5445 819.3723 1636.7301
5446 819.3723 1636.7301
5451 819.3723 1636.7301
5455 819.3723 1636.7301
5456 819.3723 1636.7301
5464 819.3723 1636.7301
5465 819.3723 1636.7301
5477 819.3723 1636.7301
5478 819.3723 1636.7301
5483 819.3723 1636.7301
5484 819.3723 1636.7301
5487 819.3723 1636.7301
5489 819.3723 1636.7301
5491 819.3723 1636.7301
5492 819.3723 1636.7301
5495 819.3723 1636.7301
5496 819.3723 1636.7301
5497 819.3723 1636.7301
5499 819.3723 1636.7301
5501 819.3723 1636.7301
5502 819.3723 1636.7301
5503 819.3723 1636.7301
5504 819.3723 1636.7301
5505 819.3723 1636.7301
5507 819.3723 1636.7301
5509 819.3723 1636.7301
5510 819.3723 1636.7301
5511 819.3723 1636.7301
5513 819.3723 1636.7301
5514 819.3723 1636.7301
5515 819.3723 1636.7301
5516 819.3723 1636.7301
5517 819.3723 1636.7301
5519 819.3723 1636.7301
5520 819.3723 1636.7301
5521 819.3723 1636.7301
5522 819.3723 1636.7301
5524 819.3723 1636.7301
5525 819.3723 1636.7301
5527 819.3723 1636.7301
5528 819.3723 1636.7301
5529 819.3723 1636.7301
5530 819.3723 1636.7301
5531 819.3723 1636.7301
5533 819.3723 1636.7301
5534 819.3723 1636.7301
5536 819.3723 1636.7301
5537 819.3723 1636.7301
5538 819.3723 1636.7301
5539 819.3723 1636.7301
5540 819.3723 1636.7301
5541 819.3723 1636.7301
5542 819.3723 1636.7301
5543 819.3723 1636.7301
5544 819.3723 1636.7301
5545 819.3723 1636.7301
5546 819.3723 1636.7301
5548 819.3723 1636.7301
5549 819.3723 1636.7301
5550 819.3723 1636.7301
5551 819.3723 1636.7301
5552 819.3723 1636.7301
5553 819.3723 1636.7301
5554 819.3723 1636.7301
5555 819.3723 1636.7301
5556 819.3723 1636.7301
5557 819.3723 1636.7301
5558 819.3723 1636.7301
5559 819.3723 1636.7301
5560 819.3723 1636.7301
5561 819.3723 1636.7301
5562 819.3723 1636.7301
5563 819.3723 1636.7301
5564 819.3723 1636.7301
5565 819.3723 1636.7301
5566 819.3723 1636.7301
5568 819.3723 1636.7301
5569 819.3723 1636.7301
5570 819.3723 1636.7301
Page: Previous 1 …54 55 56 57 58 59 60 61 62 63 64 … 76 Next 

Not what you expected? Try the select summary.

Mascot: http://www.matrixscience.com/