MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 5601–5700 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
5191 792.3600 1582.7055
5192 792.3600 1582.7055
5193 792.3600 1582.7055
5195 792.3600 1582.7055
5196 792.3600 1582.7055
5197 792.3600 1582.7055
5198 792.3600 1582.7055
5199 792.3600 1582.7055
5201 792.3600 1582.7055
5202 792.3600 1582.7055
5203 792.3600 1582.7055
5204 792.3600 1582.7055
5205 792.3600 1582.7055
5206 792.3600 1582.7055
5207 792.3600 1582.7055
5208 792.3600 1582.7055
5209 792.3600 1582.7055
5210 792.3600 1582.7055
5211 792.3600 1582.7055
5213 792.3600 2374.0583 2374.0423 6.75 0     +0Score > 21 indicates identity
Score > 13 indicates homology		 IEYVGNGYINEAQNMGWLQR + 4 Deamidated (NQ); Oxidation (M)
5214 792.3600 1582.7055
5216 792.3600 1582.7055
5217 792.3600 1582.7055
5218 792.3600 1582.7055
5219 792.3600 1582.7055
5220 792.3600 1582.7055
5221 792.3600 1582.7055
5222 792.3600 1582.7055
5223 792.3600 1582.7055
5225 792.3600 1582.7055
5226 792.3600 1582.7055
5227 792.3600 1582.7055
5228 792.3600 1582.7055
5229 792.3600 1582.7055
5230 792.3600 1582.7055
5231 792.3600 1582.7055
5232 792.3600 1582.7055
5233 792.3600 1582.7055
5234 792.3600 1582.7055
5235 792.3600 1582.7055
5236 792.3600 1582.7055
5237 792.3600 1582.7055
5238 792.3600 1582.7055
5239 792.3600 1582.7055
5240 792.3600 1582.7055
5241 792.3600 1582.7055
5242 792.3600 1582.7055
5243 792.3600 1582.7055
5244 792.3600 1582.7055
5245 792.3600 1582.7055
5246 792.3600 1582.7055
5248 792.3600 1582.7055
5249 792.3600 1582.7055
5250 792.3600 1582.7055
5251 792.3600 1582.7055
5252 792.3600 1582.7055
5253 792.3600 1582.7055
5254 792.3600 1582.7055
5255 792.3600 1582.7055
5256 805.8662 1609.7179
5257 805.8662 1609.7179
5258 805.8662 1609.7179
5259 805.8662 1609.7179
5260 805.8662 1609.7179
5261 805.8662 1609.7179
5262 805.8662 1609.7179
5263 805.8662 1609.7179
5264 805.8662 1609.7179
5265 805.8662 1609.7179
5266 805.8662 1609.7179
5267 805.8662 1609.7179
5268 805.8662 1609.7179
5269 805.8662 1609.7179
5271 805.8662 1609.7179
5274 805.8662 1609.7179
5275 805.8662 1609.7179
5277 805.8662 1609.7179
5278 805.8662 1609.7179
5279 805.8662 1609.7179
5282 805.8662 1609.7179
5283 805.8662 1609.7179
5285 805.8662 1609.7179
5287 805.8662 1609.7179
5288 805.8662 1609.7179
5289 805.8662 1609.7179
5292 805.8662 1609.7179
5293 805.8662 1609.7179
5294 805.8662 1609.7179
5295 805.8662 1609.7179
5296 805.8662 1609.7179
5297 805.8662 1609.7179
5299 805.8662 1609.7179
5300 805.8662 1609.7179
5301 805.8662 1609.7179
5302 805.8662 1609.7179
5304 805.8662 1609.7179
5305 805.8662 1609.7179
5306 805.8662 1609.7179
5307 805.8662 1609.7179
5308 805.8662 1609.7179
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