MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 5501–5600 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
5058 778.8539 1555.6932
5059 778.8539 1555.6932
5060 778.8539 1555.6932
5061 778.8539 1555.6932
5062 778.8539 1555.6932
5063 778.8539 1555.6932
5065 778.8539 1555.6932
5066 778.8539 1555.6932
5067 778.8539 1555.6932
5068 778.8539 1555.6932
5069 778.8539 1555.6932
5070 778.8539 1555.6932
5071 778.8539 1555.6932
5072 778.8539 1555.6932
5073 778.8539 1555.6932
5074 778.8539 1555.6932
5075 778.8539 1555.6932
5076 778.8539 1555.6932
5077 778.8539 1555.6932
5079 778.8539 1555.6932
5080 778.8539 1555.6932
5081 778.8539 1555.6932
5082 778.8539 1555.6932
5083 778.8539 1555.6932
5084 778.8539 1555.6932
5085 778.8539 1555.6932
5086 778.8539 1555.6932
5087 792.3600 1582.7055
5088 792.3600 1582.7055
5089 792.3600 1582.7055
5090 792.3600 1582.7055
5091 792.3600 1582.7055
5092 792.3600 1582.7055
5094 792.3600 1582.7055
5095 792.3600 1582.7055
5096 792.3600 1582.7055
5097 792.3600 1582.7055
5098 792.3600 1582.7055
5099 792.3600 1582.7055
5100 792.3600 1582.7055
5101 792.3600 1582.7055
5102 792.3600 1582.7055
5103 792.3600 1582.7055
5104 792.3600 1582.7055
5105 792.3600 1582.7055
5107 792.3600 1582.7055
5108 792.3600 1582.7055
5110 792.3600 1582.7055
5111 792.3600 1582.7055
5112 792.3600 1582.7055
5115 792.3600 1582.7055
5117 792.3600 1582.7055
5124 792.3600 1582.7055
5127 792.3600 1582.7055
5128 792.3600 1582.7055
5129 792.3600 1582.7055
5130 792.3600 1582.7055
5139 792.3600 1582.7055
5140 792.3600 1582.7055
5141 792.3600 1582.7055
5144 792.3600 1582.7055
5145 792.3600 1582.7055
5146 792.3600 1582.7055
5147 792.3600 1582.7055
5148 792.3600 1582.7055
5149 792.3600 1582.7055
5150 792.3600 1582.7055
5153 792.3600 1582.7055
5155 792.3600 1582.7055
5157 792.3600 1582.7055
5158 792.3600 1582.7055
5159 792.3600 1582.7055
5160 792.3600 1582.7055
5161 792.3600 1582.7055
5162 792.3600 1582.7055
5164 792.3600 1582.7055
5165 792.3600 1582.7055
5166 792.3600 1582.7055
5167 792.3600 1582.7055
5168 792.3600 1582.7055
5169 792.3600 1582.7055
5170 792.3600 1582.7055
5171 792.3600 1582.7055
5173 792.3600 1582.7055
5174 792.3600 1582.7055
5175 792.3600 1582.7055
5176 792.3600 1582.7055
5177 792.3600 1582.7055
5178 792.3600 1582.7055
5179 792.3600 1582.7055
5180 792.3600 1582.7055
5181 792.3600 1582.7055
5182 792.3600 1582.7055
5183 792.3600 1582.7055
5184 792.3600 1582.7055
5185 792.3600 1582.7055
5186 792.3600 1582.7055
5187 792.3600 1582.7055
5189 792.3600 1582.7055
5190 792.3600 1582.7055
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