MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 5401–5500 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4948 778.8539 1555.6932
4949 778.8539 1555.6932
4950 778.8539 1555.6932
4951 778.8539 1555.6932
4952 778.8539 1555.6932
4953 778.8539 1555.6932
4954 778.8539 1555.6932
4955 778.8539 1555.6932
4956 778.8539 1555.6932
4957 778.8539 1555.6932
4958 778.8539 1555.6932
4959 778.8539 1555.6932
4961 778.8539 1555.6932
4962 778.8539 1555.6932
4963 778.8539 1555.6932
4964 778.8539 1555.6932
4965 778.8539 1555.6932
4966 778.8539 1555.6932
4967 778.8539 1555.6932
4968 778.8539 1555.6932
4969 778.8539 1555.6932
4970 778.8539 1555.6932
4971 778.8539 1555.6932
4972 778.8539 1555.6932
4973 778.8539 1555.6932
4974 778.8539 1555.6932
4975 778.8539 1555.6932
4976 778.8539 1555.6932
4977 778.8539 1555.6932
4978 778.8539 1555.6932
4979 778.8539 1555.6932
4980 778.8539 1555.6932
4981 778.8539 1555.6932
4982 778.8539 1555.6932
4983 778.8539 1555.6932
4984 778.8539 1555.6932
4985 778.8539 1555.6932
4986 778.8539 1555.6932
4987 778.8539 1555.6932
4988 778.8539 1555.6932
4989 778.8539 1555.6932
4990 778.8539 1555.6932
4991 778.8539 1555.6932
4992 778.8539 1555.6932
4993 778.8539 1555.6932
4994 778.8539 1555.6932
4995 778.8539 1555.6932
4996 778.8539 1555.6932
4997 778.8539 1555.6932
4998 778.8539 1555.6932
4999 778.8539 1555.6932
5000 778.8539 1555.6932
5001 778.8539 1555.6932
5002 778.8539 1555.6932
5003 778.8539 1555.6932
5004 778.8539 1555.6932
5005 778.8539 1555.6932
5006 778.8539 1555.6932
5007 778.8539 1555.6932
5008 778.8539 1555.6932
5009 778.8539 1555.6932
5010 778.8539 1555.6932
5011 778.8539 1555.6932
5012 778.8539 1555.6932
5013 778.8539 1555.6932
5014 778.8539 1555.6932
5015 778.8539 1555.6932
5016 778.8539 1555.6932
5017 778.8539 1555.6932
5018 778.8539 1555.6932
5019 778.8539 1555.6932
5020 778.8539 1555.6932
5022 778.8539 1555.6932
5023 778.8539 1555.6932
5025 778.8539 1555.6932
5027 778.8539 1555.6932
5029 778.8539 1555.6932
5030 778.8539 1555.6932
5031 778.8539 1555.6932
5033 778.8539 1555.6932
5035 778.8539 1555.6932
5036 778.8539 1555.6932
5038 778.8539 1555.6932
5039 778.8539 1555.6932
5040 778.8539 1555.6932
5041 778.8539 1555.6932
5043 778.8539 1555.6932
5044 778.8539 1555.6932
5045 778.8539 1555.6932
5046 778.8539 1555.6932
5047 778.8539 1555.6932
5048 778.8539 1555.6932
5049 778.8539 1555.6932
5050 778.8539 1555.6932
5051 778.8539 1555.6932
5052 778.8539 1555.6932
5053 778.8539 1555.6932
5054 778.8539 1555.6932
5056 778.8539 1555.6932
5057 778.8539 1555.6932
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