MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 5301–5400 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4825 765.3478 1528.6810
4826 765.3478 1528.6810
4827 765.3478 1528.6810
4828 765.3478 1528.6810
4830 765.3478 1528.6810
4832 765.3478 1528.6810
4835 765.3478 1528.6810
4836 765.3478 1528.6810
4837 765.3478 1528.6810
4838 765.3478 1528.6810
4839 765.3478 1528.6810
4840 765.3478 1528.6810
4841 765.3478 1528.6810
4842 765.3478 1528.6810
4843 765.3478 1528.6810
4844 765.3478 1528.6810
4845 765.3478 1528.6810
4846 765.3478 1528.6810
4848 765.3478 1528.6810
4849 765.3478 1528.6810
4850 765.3478 1528.6810
4851 765.3478 1528.6810
4853 765.3478 1528.6810
4854 765.3478 1528.6810
4855 765.3478 1528.6810
4856 765.3478 1528.6810
4857 765.3478 1528.6810
4858 765.3478 1528.6810
4859 765.3478 1528.6810
4860 765.3478 1528.6810
4863 765.3478 1528.6810
4864 765.3478 1528.6810
4865 765.3478 1528.6810
4866 765.3478 1528.6810
4867 765.3478 1528.6810
4868 765.3478 1528.6810
4869 765.3478 1528.6810
4871 765.3478 1528.6810
4873 765.3478 1528.6810
4874 765.3478 1528.6810
4875 765.3478 1528.6810
4876 765.3478 1528.6810
4877 765.3478 1528.6810
4878 765.3478 1528.6810
4879 765.3478 1528.6810
4880 765.3478 1528.6810
4881 765.3478 1528.6810
4883 765.3478 1528.6810
4884 765.3478 1528.6810
4885 765.3478 1528.6810
4887 765.3478 1528.6810
4888 765.3478 1528.6810
4889 765.3478 1528.6810
4890 765.3478 1528.6810
4891 765.3478 1528.6810
4892 765.3478 1528.6810
4893 765.3478 1528.6810
4895 765.3478 1528.6810
4896 765.3478 1528.6810
4897 765.3478 1528.6810
4898 765.3478 1528.6810
4899 765.3478 1528.6810
4900 765.3478 1528.6810
4901 765.3478 1528.6810
4902 765.3478 1528.6810
4904 765.3478 1528.6810
4905 765.3478 1528.6810
4906 765.3478 1528.6810
4907 765.3478 1528.6810
4908 765.3478 1528.6810
4911 765.3478 1528.6810
4912 765.3478 1528.6810
4913 765.3478 1528.6810
4914 765.3478 1528.6810
4915 765.3478 1528.6810
4916 765.3478 1528.6810
4917 765.3478 1528.6810
4918 778.8539 1555.6932
4919 778.8539 1555.6932
4920 778.8539 1555.6932
4921 778.8539 1555.6932
4923 778.8539 1555.6932
4924 778.8539 1555.6932
4925 778.8539 1555.6932
4927 778.8539 1555.6932
4928 778.8539 1555.6932
4929 778.8539 1555.6932
4930 778.8539 1555.6932
4931 778.8539 1555.6932
4933 778.8539 1555.6932
4936 778.8539 1555.6932
4937 778.8539 1555.6932
4938 778.8539 1555.6932
4939 778.8539 1555.6932
4940 778.8539 1555.6932
4941 778.8539 1555.6932
4942 778.8539 1555.6932
4943 778.8539 1555.6932
4946 778.8539 1555.6932
4947 778.8539 1555.6932
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