MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 5201–5300 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4705 751.8416 1501.6687
4706 751.8416 1501.6687
4707 751.8416 1501.6687
4708 751.8416 1501.6687
4709 751.8416 1501.6687
4710 751.8416 1501.6687
4711 751.8416 1501.6687
4712 751.8416 1501.6687
4713 751.8416 1501.6687
4714 751.8416 1501.6687
4715 751.8416 1501.6687
4716 751.8416 1501.6687
4718 751.8416 1501.6687
4719 751.8416 1501.6687
4720 751.8416 1501.6687
4721 751.8416 1501.6687
4722 751.8416 1501.6687
4723 751.8416 1501.6687
4724 751.8416 1501.6687
4725 751.8416 1501.6687
4726 751.8416 1501.6687
4727 751.8416 1501.6687
4728 751.8416 1501.6687
4729 751.8416 1501.6687
4730 751.8416 1501.6687
4731 751.8416 1501.6687
4732 751.8416 1501.6687
4733 751.8416 1501.6687
4734 751.8416 1501.6687
4735 751.8416 1501.6687
4736 751.8416 1501.6687
4737 751.8416 1501.6687
4738 751.8416 1501.6687
4739 751.8416 1501.6687
4740 751.8416 1501.6687
4741 751.8416 1501.6687
4742 751.8416 1501.6687
4743 751.8416 1501.6687
4744 751.8416 1501.6687
4745 751.8416 1501.6687
4746 751.8416 1501.6687
4747 751.8416 1501.6687
4749 765.3478 1528.6810
4750 765.3478 1528.6810
4751 765.3478 1528.6810
4752 765.3478 1528.6810
4753 765.3478 1528.6810
4754 765.3478 1528.6810
4755 765.3478 1528.6810
4757 765.3478 1528.6810
4758 765.3478 1528.6810
4759 765.3478 1528.6810
4760 765.3478 1528.6810
4761 765.3478 1528.6810
4762 765.3478 1528.6810
4763 765.3478 1528.6810
4764 765.3478 1528.6810
4765 765.3478 1528.6810
4766 765.3478 1528.6810
4767 765.3478 1528.6810
4768 765.3478 1528.6810
4769 765.3478 1528.6810
4770 765.3478 1528.6810
4771 765.3478 1528.6810
4772 765.3478 1528.6810
4773 765.3478 1528.6810
4775 765.3478 1528.6810
4776 765.3478 1528.6810
4782 765.3478 1528.6810
4784 765.3478 1528.6810
4785 765.3478 1528.6810
4786 765.3478 1528.6810
4788 765.3478 1528.6810
4791 765.3478 1528.6810
4792 765.3478 1528.6810
4793 765.3478 1528.6810
4795 765.3478 1528.6810
4796 765.3478 1528.6810
4797 765.3478 1528.6810
4798 765.3478 1528.6810
4799 765.3478 1528.6810
4801 765.3478 1528.6810
4802 765.3478 1528.6810
4803 765.3478 1528.6810
4806 765.3478 1528.6810
4807 765.3478 1528.6810
4808 765.3478 1528.6810
4809 765.3478 1528.6810
4810 765.3478 1528.6810
4811 765.3478 1528.6810
4812 765.3478 1528.6810
4813 765.3478 1528.6810
4814 765.3478 1528.6810
4815 765.3478 1528.6810
4817 765.3478 1528.6810
4818 765.3478 1528.6810
4820 765.3478 1528.6810
4821 765.3478 1528.6810
4822 765.3478 1528.6810
4824 765.3478 1528.6810
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