MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 5101–5200 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4589 751.8416 1501.6687
4590 751.8416 1501.6687
4591 751.8416 1501.6687
4592 751.8416 1501.6687
4593 751.8416 1501.6687
4596 751.8416 1501.6687
4597 751.8416 1501.6687
4599 751.8416 1501.6687
4600 751.8416 1501.6687
4601 751.8416 1501.6687
4602 751.8416 1501.6687
4606 751.8416 1501.6687
4607 751.8416 1501.6687
4608 751.8416 1501.6687
4609 751.8416 1501.6687
4610 751.8416 1501.6687
4611 751.8416 1501.6687
4612 751.8416 1501.6687
4613 751.8416 1501.6687
4614 751.8416 1501.6687
4615 751.8416 1501.6687
4616 751.8416 1501.6687
4617 751.8416 1501.6687
4618 751.8416 1501.6687
4619 751.8416 1501.6687
4620 751.8416 1501.6687
4621 751.8416 1501.6687
4622 751.8416 1501.6687
4623 751.8416 1501.6687
4625 751.8416 1501.6687
4626 751.8416 1501.6687
4628 751.8416 1501.6687
4629 751.8416 1501.6687
4631 751.8416 1501.6687
4632 751.8416 1501.6687
4633 751.8416 1501.6687
4634 751.8416 1501.6687
4635 751.8416 1501.6687
4636 751.8416 1501.6687
4637 751.8416 1501.6687
4638 751.8416 1501.6687
4639 751.8416 1501.6687
4640 751.8416 1501.6687
4641 751.8416 1501.6687
4642 751.8416 1501.6687
4643 751.8416 1501.6687
4644 751.8416 1501.6687
4645 751.8416 1501.6687
4646 751.8416 1501.6687
4647 751.8416 1501.6687
4648 751.8416 1501.6687
4649 751.8416 1501.6687
4650 751.8416 1501.6687
4651 751.8416 1501.6687
4653 751.8416 1501.6687
4654 751.8416 1501.6687
4655 751.8416 1501.6687
4656 751.8416 1501.6687
4657 751.8416 1501.6687
4658 751.8416 1501.6687
4659 751.8416 1501.6687
4660 751.8416 1501.6687
4661 751.8416 1501.6687
4662 751.8416 1501.6687
4663 751.8416 1501.6687
4664 751.8416 1501.6687
4666 751.8416 1501.6687
4667 751.8416 1501.6687
4668 751.8416 1501.6687
4669 751.8416 1501.6687
4670 751.8416 1501.6687
4671 751.8416 1501.6687
4672 751.8416 1501.6687
4673 751.8416 1501.6687
4674 751.8416 1501.6687
4675 751.8416 1501.6687
4676 751.8416 1501.6687
4677 751.8416 1501.6687
4678 751.8416 1501.6687
4679 751.8416 1501.6687
4680 751.8416 1501.6687
4681 751.8416 1501.6687
4682 751.8416 1501.6687
4683 751.8416 1501.6687
4684 751.8416 1501.6687
4685 751.8416 1501.6687
4687 751.8416 1501.6687
4689 751.8416 1501.6687
4692 751.8416 1501.6687
4693 751.8416 1501.6687
4694 751.8416 1501.6687
4695 751.8416 1501.6687
4696 751.8416 1501.6687
4697 751.8416 1501.6687
4698 751.8416 1501.6687
4699 751.8416 1501.6687
4700 751.8416 1501.6687
4701 751.8416 1501.6687
4702 751.8416 1501.6687
4704 751.8416 1501.6687
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