MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 5001–5100 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4472 738.3355 1474.6565
4475 738.3355 1474.6565
4476 738.3355 1474.6565
4478 738.3355 1474.6565
4480 738.3355 1474.6565
4481 738.3355 1474.6565
4483 738.3355 1474.6565
4484 738.3355 1474.6565
4485 738.3355 1474.6565
4487 738.3355 1474.6565
4488 738.3355 1474.6565
4489 738.3355 1474.6565
4490 738.3355 1474.6565
4492 738.3355 1474.6565
4493 738.3355 1474.6565
4494 738.3355 1474.6565
4495 738.3355 1474.6565
4496 738.3355 1474.6565
4497 738.3355 1474.6565
4498 738.3355 1474.6565
4499 738.3355 1474.6565
4500 738.3355 1474.6565
4501 738.3355 1474.6565
4502 738.3355 1474.6565
4503 738.3355 1474.6565
4504 738.3355 1474.6565
4505 738.3355 1474.6565
4506 738.3355 1474.6565
4507 738.3355 1474.6565
4508 738.3355 1474.6565
4509 738.3355 1474.6565
4510 738.3355 1474.6565
4511 738.3355 1474.6565
4512 738.3355 1474.6565
4513 738.3355 1474.6565
4514 738.3355 1474.6565
4515 738.3355 1474.6565
4517 738.3355 1474.6565
4518 738.3355 1474.6565
4519 738.3355 1474.6565
4521 738.3355 1474.6565
4522 738.3355 1474.6565
4524 738.3355 1474.6565
4525 738.3355 1474.6565
4526 738.3355 1474.6565
4527 738.3355 1474.6565
4528 738.3355 1474.6565
4529 738.3355 1474.6565
4530 738.3355 1474.6565
4531 738.3355 1474.6565
4532 738.3355 1474.6565
4533 738.3355 1474.6565
4535 738.3355 1474.6565
4537 738.3355 1474.6565
4538 738.3355 1474.6565
4539 738.3355 1474.6565
4540 738.3355 1474.6565
4541 738.3355 1474.6565
4543 738.3355 1474.6565
4544 738.3355 1474.6565
4545 738.3355 1474.6565
4546 738.3355 1474.6565
4547 738.3355 1474.6565
4548 738.3355 1474.6565
4549 738.3355 1474.6565
4551 738.3355 1474.6565
4552 738.3355 1474.6565
4553 738.3355 1474.6565
4555 738.3355 1474.6565
4556 738.3355 1474.6565
4557 738.3355 1474.6565
4559 738.3355 1474.6565
4560 738.3355 1474.6565
4561 738.3355 1474.6565
4562 738.3355 1474.6565
4563 738.3355 1474.6565
4564 738.3355 1474.6565
4565 738.3355 1474.6565
4567 738.3355 1474.6565
4568 738.3355 1474.6565
4569 738.3355 1474.6565
4570 738.3355 1474.6565
4571 738.3355 1474.6565
4572 738.3355 1474.6565
4573 738.3355 1474.6565
4574 738.3355 1474.6565
4575 738.3355 1474.6565
4576 738.3355 1474.6565
4577 738.3355 1474.6565
4578 738.3355 1474.6565
4579 738.3355 1474.6565
4580 751.8416 1501.6687
4581 751.8416 1501.6687
4582 751.8416 1501.6687
4583 751.8416 1501.6687
4584 751.8416 1501.6687
4585 751.8416 1501.6687
4586 751.8416 1501.6687
4587 751.8416 1501.6687
4588 751.8416 1501.6687
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