MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4901–5000 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4340 724.8293 1447.6441
4341 724.8293 1447.6441
4342 724.8293 1447.6441
4343 724.8293 1447.6441
4344 724.8293 1447.6441
4345 724.8293 1447.6441
4346 724.8293 1447.6441
4347 724.8293 1447.6441
4348 724.8293 1447.6441
4350 724.8293 1447.6441
4351 724.8293 1447.6441
4352 724.8293 1447.6441
4359 724.8293 1447.6441
4361 724.8293 1447.6441
4362 724.8293 1447.6441
4365 724.8293 1447.6441
4367 724.8293 1447.6441
4368 724.8293 1447.6441
4369 724.8293 1447.6441
4370 724.8293 1447.6441
4371 724.8293 1447.6441
4372 724.8293 1447.6441
4373 724.8293 1447.6441
4374 724.8293 1447.6441
4375 724.8293 1447.6441
4377 724.8293 1447.6441
4378 724.8293 1447.6441
4379 724.8293 1447.6441
4380 724.8293 1447.6441
4381 724.8293 1447.6441
4382 724.8293 1447.6441
4383 724.8293 1447.6441
4384 724.8293 1447.6441
4385 724.8293 1447.6441
4386 724.8293 1447.6441
4387 724.8293 1447.6441
4388 724.8293 1447.6441
4389 724.8293 1447.6441
4390 724.8293 1447.6441
4391 724.8293 1447.6441
4392 724.8293 1447.6441
4393 724.8293 1447.6441
4394 724.8293 1447.6441
4395 724.8293 1447.6441
4396 724.8293 1447.6441
4397 724.8293 1447.6441
4398 724.8293 1447.6441
4399 724.8293 1447.6441
4400 724.8293 1447.6441
4401 724.8293 1447.6441
4402 724.8293 1447.6441
4403 724.8293 1447.6441
4404 724.8293 1447.6441
4405 724.8293 1447.6441
4406 724.8293 1447.6441
4407 724.8293 1447.6441
4408 724.8293 1447.6441
4409 724.8293 1447.6441
4410 724.8293 1447.6441
4411 738.3355 1474.6565
4412 738.3355 1474.6565
4413 738.3355 1474.6565
4414 738.3355 1474.6565
4415 738.3355 1474.6565
4416 738.3355 1474.6565
4417 738.3355 1474.6565
4418 738.3355 1474.6565
4419 738.3355 1474.6565
4420 738.3355 1474.6565
4421 738.3355 1474.6565
4422 738.3355 1474.6565
4423 738.3355 1474.6565
4424 738.3355 1474.6565
4425 738.3355 1474.6565
4426 738.3355 1474.6565
4427 738.3355 1474.6565
4428 738.3355 1474.6565
4429 738.3355 1474.6565
4430 738.3355 1474.6565
4435 738.3355 1474.6565
4436 738.3355 1474.6565
4437 738.3355 1474.6565
4441 738.3355 1474.6565
4450 738.3355 1474.6565
4451 738.3355 1474.6565
4452 738.3355 1474.6565
4453 738.3355 1474.6565
4454 738.3355 1474.6565
4459 738.3355 1474.6565
4460 738.3355 1474.6565
4461 738.3355 1474.6565
4462 738.3355 1474.6565
4463 738.3355 1474.6565
4464 738.3355 1474.6565
4465 738.3355 1474.6565
4466 738.3355 1474.6565
4467 738.3355 1474.6565
4468 738.3355 1474.6565
4469 738.3355 1474.6565
4471 738.3355 1474.6565
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