MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 401–500 (out of 7566)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
577 427.6943 853.3741 853.3600 16.5 0 5 0.66 +1Score > 16 indicates identity MTSSAGQR + Deamidated (NQ); Oxidation (M)
1851 522.2372 2084.9199 2084.9361 -7.77 0 5 1.5 +1Score > 19 indicates identity SDNCEDTPEAGYFAIAVVK
3928 697.8171 2787.2392 2787.2612 -7.90 0 5 1.5 +1Score > 19 indicates identity QHHLWASQGSDFHQPCPWIELGR + 2 Deamidated (NQ)
449 414.1881 1652.7235 1652.7278 -2.62 0 5 1.5 +1Score > 19 indicates identity AYSGEGAIADDAGNVSR + Deamidated (NQ)
1577 508.7311 2030.8955 2030.9190 -11.6 1 5 0.86 +1Score > 17 indicates identity GRVYAVSMFNPQGNDMTK + Deamidated (NQ); Oxidation (M)
633 427.6943 853.3741 853.3851 -12.9 1 5 0.68 +1Score > 16 indicates identity MSSKEQK + Deamidated (NQ); Oxidation (M)
1563 508.7311 2030.8955 2030.8979 -1.19 0 5 0.86 +1Score > 17 indicates identity MSMGHEVVGHWFNEEVK + Oxidation (M)
2874 603.2741 1204.5337 1204.5394 -4.73 0 5 1 +1Score > 20 indicates identity
Score > 18 indicates homology
ADCEQLAELR + Deamidated (NQ)
3836 684.3109 1366.6073 1366.6266 -14.1 1 5 0.52 +1Score > 20 indicates identity
Score > 15 indicates homology
DWRETNIDYR
5744 832.8785 3327.4848 3327.5452 -18.2 1 5 1.5 +1Score > 19 indicates identity IQTWQYSDSQQAISIEDNGCGIDLSLQKK + 3 Deamidated (NQ)
488 414.1881 826.3617 826.3677 -7.23 0 5 1.5 +1Score > 19 indicates identity MATVSMR + 2 Oxidation (M)
1387 495.2250 988.4354 988.4219 13.7 0 5 1 +1Score > 20 indicates identity
Score > 18 indicates homology
GNHEVMMR + Oxidation (M)
3445 657.2986 1312.5827 1312.5751 5.80 0 5 0.64 +1Score > 21 indicates identity
Score > 16 indicates homology
NMVQNMLSSTR + Deamidated (NQ); 2 Oxidation (M)
3471 657.2986 1968.8741 1968.8809 -3.43 0 5 0.39 +1Score > 21 indicates identity
Score > 13 indicates homology
YVLGDMLGQSPQMEQVR + 3 Deamidated (NQ); Oxidation (M)
5493 819.3723 2455.0951 2455.1308 -14.5 1 5 1 +1Score > 21 indicates identity
Score > 18 indicates homology
QTPMRGHPVFIAQHATATCCR + Deamidated (NQ); Oxidation (M)
975 454.7066 1814.7972 1814.7846 6.94 0 5 1 +1Score > 17 indicates identity GFAAEGQSQNNYLNGLK + 5 Deamidated (NQ)
2616 589.7679 2355.0427 2355.0518 -3.86 1 5 1.4 +1Score > 19 indicates identity NPVFWEKMNNGWDEFSIPK + 2 Deamidated (NQ); Oxidation (M)
563 427.6943 1706.7481 1706.7610 -7.56 0 5 0.68 +1Score > 16 indicates identity WDTLEDVWNGLCAK + Deamidated (NQ)
1051 468.2127 1868.8217 1868.8356 -7.44 1 5 1 +1Score > 20 indicates identity
Score > 17 indicates homology
MSANTEAQGSGRGLEAMK + 2 Oxidation (M)
4096 711.3232 2130.9479 2130.9124 16.7 0 5 1 +1Score > 21 indicates identity
Score > 17 indicates homology
NNTVYSAATQTGVCQGDTSR + 2 Deamidated (NQ)
4703 751.8416 1501.6687 1501.6620 4.45 1 5 1.4 +1Score > 19 indicates identity FDDRGDLSFMQR + Oxidation (M)
795 441.2004 1320.5795 1320.5833 -2.90 0 5 1 +1Score > 20 indicates identity
Score > 17 indicates homology
ILQYGNENPNR + 4 Deamidated (NQ)
2906 616.7802 2463.0917 2463.0934 -0.66 0 5 1.2 +1Score > 18 indicates identity MAQITTTDANEFSSSAEFIPMR + Deamidated (NQ); Oxidation (M)
3701 670.8048 1339.5951 1339.6190 -17.9 1 5 1.5 +1Score > 19 indicates identity KDSGFQMNQLR + Deamidated (NQ); Oxidation (M)
5462 819.3723 1636.7301 1636.7303 -0.17 1 5 0.63 +1Score > 21 indicates identity
Score > 15 indicates homology
WQNMNENRTLWK + 2 Deamidated (NQ); Oxidation (M)
819 441.2004 1320.5795 1320.6020 -17.0 0 5 0.62 +1Score > 20 indicates identity
Score > 15 indicates homology
DGIPLMEEDFR
2172 549.2496 1096.4846 1096.4753 8.44 1 5 1 +1Score > 20 indicates identity
Score > 17 indicates homology
GDARMMASSR + Oxidation (M)
96 387.1759 1158.5058 1158.5088 -2.56 1 5 0.78 +1Score > 16 indicates identity RMADEHGTDK
408 414.1881 1652.7235 1652.7069 10.0 1 5 1.5 +1Score > 19 indicates identity RMGELMAESHASMR + 3 Oxidation (M)
1782 522.2372 1563.6899 1563.6771 8.17 0 5 1.6 +1Score > 19 indicates identity MMFEFNMAELLR + Deamidated (NQ); 2 Oxidation (M)
2230 562.7557 1123.4968 1123.4824 12.8 1 5 1.2 +1Score > 18 indicates identity RQMMGMEPK + Deamidated (NQ); Oxidation (M)
3472 657.2986 1968.8741 1968.8809 -3.43 0 5 0.91 +1Score > 21 indicates identity
Score > 17 indicates homology
YVLGDMLGQSPQMEQVR + 3 Deamidated (NQ); Oxidation (M)
7151 954.4337 3813.7058 3813.7145 -2.29 0 5 1 +1Score > 21 indicates identity
Score > 17 indicates homology
NGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMR + 2 Deamidated (NQ)
2830 603.2741 1204.5337 1204.5394 -4.75 1 5 1 +1Score > 20 indicates identity
Score > 17 indicates homology
GDLKNMNPDGK + Deamidated (NQ); Oxidation (M)
3226 630.2864 2517.1164 2517.1482 -12.6 1 5 1 +1Score > 20 indicates identity
Score > 17 indicates homology
ENAGYVIYECYQPDDGDKLIR
93 387.1759 1158.5058 1158.5267 -18.0 0 5 0.79 +1Score > 16 indicates identity FMAQYQELK + 2 Deamidated (NQ)
3820 684.3109 2049.9109 2049.9479 -18.0 1 5 0.41 +1Score > 20 indicates identity
Score > 14 indicates homology
IEHYHDWLRDAHAMEK
5475 819.3723 3273.4602 3273.5036 -13.3 1 5 1 +1Score > 21 indicates identity
Score > 17 indicates homology
ACTQAAEVALFTYNSRAAQIWWQQNQSK + 4 Deamidated (NQ)
6145 873.3969 2617.1689 2617.2118 -16.4 1 5 1 +1Score > 22 indicates identity
Score > 17 indicates homology
GDLLELYCGNGNFSLALARNFDR + 3 Deamidated (NQ)
505 414.1881 1652.7235 1652.7069 10.0 1 5 1.5 +1Score > 19 indicates identity RMGELMAESHASMR + 3 Oxidation (M)
6485 900.4092 1798.8038 1798.8151 -6.27 0 5 0.83 +1Score > 20 indicates identity
Score > 17 indicates homology
VMQVSAMADQMLLDSK + Deamidated (NQ); 2 Oxidation (M)
6524 900.4092 3597.6076 3597.5545 14.8 0 5 1 +1Score > 20 indicates identity
Score > 17 indicates homology
GSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR + 3 Deamidated (NQ); 2 Oxidation (M)
3994 697.8171 2787.2392 2787.1972 15.1 1 5 1.6 +1Score > 19 indicates identity MERGIDLTAMMAQGITMGDEFHQR + 2 Deamidated (NQ); 3 Oxidation (M)
6764 913.9153 1825.8160 1825.8438 -15.2 0 5 1.5 +1Score > 19 indicates identity LDDMLEIQTEITSMR + 2 Oxidation (M)
586 427.6943 1706.7481 1706.7280 11.8 0 5 0.7 +1Score > 16 indicates identity FIEENHENIMACAK + 2 Deamidated (NQ)
198 400.6820 799.3495 799.3422 9.09 0 5 0.74 +1Score > 16 indicates identity FTQMQK + 2 Deamidated (NQ); Oxidation (M)
470 414.1881 1239.5426 1239.5661 -19.0 0 5 1.5 +1Score > 19 indicates identity SPMELMTMLR + 2 Oxidation (M)
4094 711.3232 2841.2639 2841.3003 -12.8 1 5 0.7 +1Score > 21 indicates identity
Score > 16 indicates homology
AKNGDNEYMAWDMTLGWIIWQQR + Oxidation (M)
237 400.6820 799.3495 799.3460 4.28 0 5 0.74 +1Score > 16 indicates identity ATDDHNK
391 414.1881 1652.7235 1652.7253 -1.09 0 5 1.5 +1Score > 19 indicates identity CWQEEAYAEAQLR
675 427.6943 1706.7481 1706.7437 2.58 0 5 0.71 +1Score > 16 indicates identity QWVNNGFAGWNGQAR + 3 Deamidated (NQ)
1516 495.2250 1482.6531 1482.6443 5.96 1 5 1 +1Score > 20 indicates identity
Score > 17 indicates homology
MSGDNGMTEEKLR + Oxidation (M)
3249 643.7925 1285.5705 1285.5761 -4.34 0 5 1.3 +1Score > 18 indicates identity MQAYFDQLDR
738 441.2004 880.3863 880.3960 -11.0 0 5 0.81 +1Score > 20 indicates identity
Score > 16 indicates homology
DSLMSANK + Oxidation (M)
4443 738.3355 1474.6565 1474.6524 2.78 1 5 1 +1Score > 20 indicates identity
Score > 17 indicates homology
NMAWRHNPNYR + Deamidated (NQ); Oxidation (M)
136 387.1759 772.3372 772.3464 -11.9 0 5 0.81 +1Score > 16 indicates identity NNPDGTR
7425 967.9399 1933.8652 1933.8298 18.3 1 5 1.6 +1Score > 19 indicates identity QSMGFDTNMEGGFLARR + 2 Deamidated (NQ); Oxidation (M)
51 387.1759 1544.6744 1544.6594 9.70 0 5 0.82 +1Score > 16 indicates identity MQEALMSLMQMAK + 2 Deamidated (NQ); 2 Oxidation (M)
1134 468.2127 934.4109 934.4290 -19.5 1 5 1 +1Score > 20 indicates identity
Score > 17 indicates homology
DCSLQRR + Deamidated (NQ)
2436 576.2618 1725.7637 1725.7742 -6.09 1 5 1 +1Score > 20 indicates identity
Score > 17 indicates homology
GMVNNKFNYFIMSK + 2 Deamidated (NQ); 2 Oxidation (M)
1917 535.7434 2138.9445 2138.9716 -12.7 1 5 1.1 +1Score > 18 indicates identity ITGSQVKNNGYVQDTNNQR + 4 Deamidated (NQ)
1919 535.7434 2138.9445 2138.9716 -12.7 1 5 1.1 +1Score > 18 indicates identity ITGSQVKNNGYVQDTNNQR + 4 Deamidated (NQ)
639 427.6943 1706.7481 1706.7318 9.54 1 5 0.72 +1Score > 16 indicates identity WDTLDDDRANGCIR + Deamidated (NQ)
5476 819.3723 1636.7301 1636.7549 -15.2 0 5 1 +1Score > 21 indicates identity
Score > 17 indicates homology
MQTQMQTQQIQQK + Deamidated (NQ); Oxidation (M)
2101 549.2496 1096.4846 1096.4753 8.44 1 5 1 +1Score > 20 indicates identity
Score > 17 indicates homology
GDARMMASSR + Oxidation (M)
6173 873.3969 1744.7793 1744.7904 -6.37 0 5 0.39 +1Score > 22 indicates identity
Score > 13 indicates homology
DIDVDYASHSPQIER + Deamidated (NQ)
4439 738.3355 1474.6565 1474.6423 9.59 0 5 1 +1Score > 20 indicates identity
Score > 17 indicates homology
SETAQPSDQALAQQ + 2 Deamidated (NQ)
4456 738.3355 2949.3129 2949.3499 -12.5 0 5 0.38 +1Score > 20 indicates identity
Score > 13 indicates homology
MNNAFMMHASTSPFYPLFAALNINAK + Deamidated (NQ); 3 Oxidation (M)
1800 522.2372 1563.6899 1563.6771 8.17 0 5 1.6 +1Score > 19 indicates identity MMFEFNMAELLR + Deamidated (NQ); 2 Oxidation (M)
2079 549.2496 1096.4846 1096.5036 -17.4 1 5 1 +1Score > 20 indicates identity
Score > 17 indicates homology
KYGPDSSTNK + Deamidated (NQ)
4935 778.8539 1555.6932 1555.6759 11.1 0 5 1.3 +1Score > 18 indicates identity QQVEGASAMGQMFR + Deamidated (NQ); Oxidation (M)
26 387.1759 1544.6744 1544.6594 9.70 0 5 0.84 +1Score > 16 indicates identity MQEALMSLMQMAK + 2 Deamidated (NQ); 2 Oxidation (M)
3873 684.3109 1366.6073 1366.5857 15.8 0 5 1 +1Score > 20 indicates identity
Score > 17 indicates homology
EQQLEVMTGCR + Deamidated (NQ); Oxidation (M)
155 387.1759 1544.6744 1544.6705 2.55 0 5 0.84 +1Score > 16 indicates identity QDDAMVAFQNFIK + 3 Deamidated (NQ); Oxidation (M)
896 454.7066 907.3986 907.3817 18.6 1 5 1.1 +1Score > 17 indicates identity RDNQAMR + 2 Deamidated (NQ); Oxidation (M)
2780 603.2741 1806.8005 1806.8314 -17.1 0 5 1 +1Score > 20 indicates identity
Score > 17 indicates homology
TVAAEMPAMQCLQLAR + 2 Deamidated (NQ); Oxidation (M)
504 414.1881 1239.5426 1239.5367 4.73 0 5 1.6 +1Score > 19 indicates identity TEQQYENATR + Deamidated (NQ)
600 427.6943 853.3741 853.3608 15.5 0 5 0.74 +1Score > 16 indicates identity MMSQAMR
3243 643.7925 1285.5705 1285.5795 -6.95 0 5 1.4 +1Score > 18 indicates identity MVTNLYQNMR + Deamidated (NQ); Oxidation (M)
4886 765.3478 3057.3620 3057.3972 -11.5 0 5 1 +1Score > 21 indicates identity
Score > 17 indicates homology
MANWLNQLQSLLGQSSSSTSSSADQGLVK + 5 Deamidated (NQ); Oxidation (M)
1460 495.2250 988.4354 988.4461 -10.9 0 5 0.9 +1Score > 20 indicates identity
Score > 17 indicates homology
VNADPESTR + Deamidated (NQ)
1674 508.7311 2030.8955 2030.8747 10.2 1 5 0.95 +1Score > 17 indicates identity RFGMMLGQGMDVQSAQEK + 3 Deamidated (NQ); Oxidation (M)
1381 495.2250 1482.6531 1482.6748 -14.6 0 5 0.55 +1Score > 20 indicates identity
Score > 14 indicates homology
AFEVGCTLNWMR
2533 576.2618 1150.5091 1150.5288 -17.1 0 5 1 +1Score > 20 indicates identity
Score > 17 indicates homology
TGDIMTIGGNR + Deamidated (NQ); Oxidation (M)
888 454.7066 907.3986 907.3891 10.4 0 5 1.1 +1Score > 17 indicates identity DMAMNQAK
596 427.6943 853.3741 853.3851 -13.0 0 5 0.75 +1Score > 16 indicates identity GEMSTVSK + Oxidation (M)
6712 913.9153 1825.8160 1825.8087 4.01 1 5 1.6 +1Score > 19 indicates identity NGMECGIGVKNYNDVR + Deamidated (NQ)
6210 873.3969 3489.5585 3489.6279 -19.9 0 5 1 +1Score > 22 indicates identity
Score > 17 indicates homology
EGQHLMLEIEDNAGLYQPVTNASGLGMNLVDK + 2 Deamidated (NQ); 2 Oxidation (M)
489 414.1881 1239.5426 1239.5441 -1.22 0 5 1.6 +1Score > 19 indicates identity AYCNLNEGIGK + 2 Deamidated (NQ)
972 454.7066 1814.7972 1814.8179 -11.4 1 5 1.1 +1Score > 17 indicates identity ALTFCEAEMDNLTRK + Deamidated (NQ); Oxidation (M)
1450 495.2250 988.4354 988.4219 13.7 0 5 0.65 +1Score > 20 indicates identity
Score > 15 indicates homology
GNHEVMMR + Oxidation (M)
3754 684.3109 2049.9109 2049.9500 -19.0 1 5 0.41 +1Score > 20 indicates identity
Score > 13 indicates homology
QDAVQHAQQLLKMMGYGV + 2 Deamidated (NQ); 2 Oxidation (M)
1519 495.2250 1482.6531 1482.6443 5.96 1 5 0.57 +1Score > 20 indicates identity
Score > 15 indicates homology
MSGDNGMTEEKLR + Oxidation (M)
4448 738.3355 1474.6565 1474.6582 -1.21 1 5 0.89 +1Score > 20 indicates identity
Score > 17 indicates homology
AECQNQQGNLRR + 2 Deamidated (NQ)
2433 576.2618 1725.7637 1725.7707 -4.04 1 5 0.97 +1Score > 20 indicates identity
Score > 17 indicates homology
YHVNQYTGDESRTR + Deamidated (NQ)
1058 468.2127 1401.6163 1401.6194 -2.24 0 5 1 +1Score > 20 indicates identity
Score > 17 indicates homology
TLPPGCEGEEASR
2089 549.2496 2192.9692 2193.0017 -14.8 1 5 0.57 +1Score > 20 indicates identity
Score > 15 indicates homology
IAFVCDAGMGSSAMGATTFRK + Oxidation (M)
2239 562.7557 1123.4968 1123.5179 -18.8 0 5 1.3 +1Score > 18 indicates identity SASLSDIEMR + Oxidation (M)
4779 765.3478 3057.3620 3057.3994 -12.2 1 5 1 +1Score > 21 indicates identity
Score > 17 indicates homology
FASYLIAAVQQATNGHCAICETMAQKR + 3 Deamidated (NQ); Oxidation (M)
5784 846.3846 2536.1319 2536.1322 -0.10 0 5 1 +1Score > 20 indicates identity
Score > 17 indicates homology
AASMITESQCYQLEQNLHQQR + 2 Deamidated (NQ)
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