MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4801–4900 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4225 711.3232 1420.6319
4227 711.3232 1420.6319
4228 711.3232 1420.6319
4229 711.3232 1420.6319
4230 711.3232 1420.6319
4231 711.3232 1420.6319
4232 711.3232 1420.6319
4233 711.3232 1420.6319
4234 711.3232 1420.6319
4235 711.3232 1420.6319
4236 711.3232 1420.6319
4237 711.3232 1420.6319
4238 711.3232 1420.6319
4239 711.3232 1420.6319
4240 711.3232 1420.6319
4241 711.3232 1420.6319
4242 724.8293 1447.6441
4243 724.8293 1447.6441
4244 724.8293 1447.6441
4245 724.8293 1447.6441
4246 724.8293 1447.6441
4247 724.8293 1447.6441
4248 724.8293 1447.6441
4249 724.8293 1447.6441
4250 724.8293 1447.6441
4252 724.8293 1447.6441
4256 724.8293 1447.6441
4257 724.8293 1447.6441
4258 724.8293 1447.6441
4261 724.8293 1447.6441
4263 724.8293 1447.6441
4264 724.8293 1447.6441
4265 724.8293 1447.6441
4266 724.8293 1447.6441
4268 724.8293 1447.6441
4269 724.8293 1447.6441
4270 724.8293 1447.6441
4271 724.8293 1447.6441
4272 724.8293 1447.6441
4273 724.8293 1447.6441
4274 724.8293 1447.6441
4275 724.8293 1447.6441
4276 724.8293 1447.6441
4277 724.8293 1447.6441
4278 724.8293 1447.6441
4279 724.8293 1447.6441
4281 724.8293 1447.6441
4282 724.8293 1447.6441
4284 724.8293 1447.6441
4286 724.8293 1447.6441
4287 724.8293 1447.6441
4288 724.8293 1447.6441
4289 724.8293 1447.6441
4290 724.8293 1447.6441
4291 724.8293 1447.6441
4292 724.8293 1447.6441
4293 724.8293 1447.6441
4294 724.8293 1447.6441
4295 724.8293 1447.6441
4296 724.8293 1447.6441
4297 724.8293 1447.6441
4298 724.8293 1447.6441
4299 724.8293 1447.6441
4300 724.8293 1447.6441
4301 724.8293 1447.6441
4302 724.8293 1447.6441
4303 724.8293 1447.6441
4304 724.8293 1447.6441
4306 724.8293 1447.6441
4307 724.8293 1447.6441
4308 724.8293 1447.6441
4309 724.8293 1447.6441
4310 724.8293 1447.6441
4311 724.8293 1447.6441
4312 724.8293 1447.6441
4313 724.8293 1447.6441
4315 724.8293 1447.6441
4317 724.8293 1447.6441
4318 724.8293 1447.6441
4319 724.8293 1447.6441
4320 724.8293 1447.6441
4321 724.8293 1447.6441
4322 724.8293 1447.6441
4323 724.8293 1447.6441
4324 724.8293 1447.6441
4325 724.8293 1447.6441
4326 724.8293 1447.6441
4327 724.8293 1447.6441
4328 724.8293 1447.6441
4329 724.8293 1447.6441
4330 724.8293 1447.6441
4331 724.8293 1447.6441
4332 724.8293 1447.6441
4333 724.8293 1447.6441
4334 724.8293 1447.6441
4335 724.8293 1447.6441
4336 724.8293 1447.6441
4337 724.8293 1447.6441
4338 724.8293 1447.6441
4339 724.8293 1447.6441
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