MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4701–4800 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4086 711.3232 1420.6319
4087 711.3232 1420.6319
4088 711.3232 1420.6319
4090 711.3232 1420.6319
4091 711.3232 1420.6319
4099 711.3232 1420.6319
4100 711.3232 1420.6319
4105 711.3232 1420.6319
4108 711.3232 1420.6319
4110 711.3232 1420.6319
4111 711.3232 1420.6319
4112 711.3232 1420.6319
4113 711.3232 1420.6319
4118 711.3232 1420.6319
4119 711.3232 1420.6319
4120 711.3232 1420.6319
4122 711.3232 1420.6319
4124 711.3232 1420.6319
4126 711.3232 1420.6319
4127 711.3232 1420.6319
4129 711.3232 1420.6319
4130 711.3232 1420.6319
4132 711.3232 1420.6319
4134 711.3232 1420.6319
4135 711.3232 1420.6319
4136 711.3232 1420.6319
4138 711.3232 1420.6319
4139 711.3232 1420.6319
4140 711.3232 1420.6319
4141 711.3232 1420.6319
4142 711.3232 1420.6319
4145 711.3232 1420.6319
4146 711.3232 1420.6319
4147 711.3232 1420.6319
4148 711.3232 1420.6319
4150 711.3232 1420.6319
4151 711.3232 1420.6319
4152 711.3232 1420.6319
4155 711.3232 1420.6319
4157 711.3232 1420.6319
4158 711.3232 1420.6319
4159 711.3232 1420.6319
4160 711.3232 1420.6319
4161 711.3232 1420.6319
4162 711.3232 1420.6319
4163 711.3232 1420.6319
4164 711.3232 1420.6319
4165 711.3232 1420.6319
4167 711.3232 1420.6319
4168 711.3232 1420.6319
4169 711.3232 1420.6319
4170 711.3232 1420.6319
4171 711.3232 1420.6319
4172 711.3232 1420.6319
4173 711.3232 1420.6319
4174 711.3232 1420.6319
4175 711.3232 1420.6319
4176 711.3232 1420.6319
4177 711.3232 1420.6319
4178 711.3232 1420.6319
4179 711.3232 1420.6319
4180 711.3232 1420.6319
4182 711.3232 1420.6319
4183 711.3232 1420.6319
4184 711.3232 1420.6319
4185 711.3232 1420.6319
4186 711.3232 1420.6319
4187 711.3232 1420.6319
4188 711.3232 1420.6319
4189 711.3232 1420.6319
4190 711.3232 1420.6319
4191 711.3232 1420.6319
4192 711.3232 1420.6319
4194 711.3232 1420.6319
4195 711.3232 1420.6319
4196 711.3232 1420.6319
4197 711.3232 1420.6319
4198 711.3232 1420.6319
4199 711.3232 1420.6319
4200 711.3232 1420.6319
4201 711.3232 1420.6319
4202 711.3232 1420.6319
4203 711.3232 1420.6319
4204 711.3232 1420.6319
4205 711.3232 1420.6319
4207 711.3232 1420.6319
4208 711.3232 1420.6319
4209 711.3232 1420.6319
4210 711.3232 1420.6319
4211 711.3232 1420.6319
4212 711.3232 1420.6319
4213 711.3232 1420.6319
4214 711.3232 1420.6319
4215 711.3232 1420.6319
4217 711.3232 1420.6319
4218 711.3232 1420.6319
4219 711.3232 1420.6319
4221 711.3232 1420.6319
4222 711.3232 1420.6319
4223 711.3232 1420.6319
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