MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4501–4600 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3840 684.3109 1366.6073
3841 684.3109 1366.6073
3842 684.3109 1366.6073
3843 684.3109 1366.6073
3844 684.3109 1366.6073
3845 684.3109 1366.6073
3846 684.3109 1366.6073
3848 684.3109 1366.6073
3849 684.3109 1366.6073
3851 684.3109 1366.6073
3852 684.3109 1366.6073
3853 684.3109 1366.6073
3854 684.3109 1366.6073
3855 684.3109 1366.6073
3856 684.3109 1366.6073
3857 684.3109 1366.6073
3859 684.3109 1366.6073
3860 684.3109 1366.6073
3862 684.3109 1366.6073
3863 684.3109 1366.6073
3864 684.3109 1366.6073
3866 684.3109 1366.6073
3867 684.3109 1366.6073
3868 684.3109 1366.6073
3869 684.3109 1366.6073
3870 684.3109 1366.6073
3871 684.3109 1366.6073
3872 684.3109 1366.6073
3874 684.3109 1366.6073
3875 684.3109 1366.6073
3876 684.3109 1366.6073
3877 684.3109 1366.6073
3878 684.3109 1366.6073
3879 684.3109 1366.6073
3880 684.3109 1366.6073
3881 684.3109 1366.6073
3882 684.3109 1366.6073
3883 684.3109 1366.6073
3885 684.3109 1366.6073
3886 684.3109 1366.6073
3888 684.3109 1366.6073
3891 684.3109 1366.6073
3892 684.3109 1366.6073
3893 684.3109 1366.6073
3894 684.3109 1366.6073
3895 684.3109 1366.6073
3896 684.3109 1366.6073
3897 684.3109 1366.6073
3898 684.3109 1366.6073
3899 684.3109 1366.6073
3900 684.3109 1366.6073
3901 684.3109 1366.6073
3902 684.3109 1366.6073
3903 684.3109 1366.6073
3904 697.8171 1393.6196
3905 697.8171 1393.6196
3906 697.8171 1393.6196
3907 697.8171 1393.6196
3908 697.8171 1393.6196
3909 697.8171 1393.6196
3911 697.8171 1393.6196
3912 697.8171 1393.6196
3913 697.8171 1393.6196
3914 697.8171 1393.6196
3915 697.8171 1393.6196
3921 697.8171 1393.6196
3924 697.8171 1393.6196
3925 697.8171 1393.6196
3929 697.8171 1393.6196
3930 697.8171 1393.6196
3931 697.8171 1393.6196
3932 697.8171 1393.6196
3933 697.8171 1393.6196
3935 697.8171 1393.6196
3936 697.8171 1393.6196
3937 697.8171 1393.6196
3938 697.8171 1393.6196
3939 697.8171 1393.6196
3940 697.8171 1393.6196
3941 697.8171 1393.6196
3942 697.8171 1393.6196
3943 697.8171 1393.6196
3944 697.8171 1393.6196
3946 697.8171 1393.6196
3947 697.8171 1393.6196
3948 697.8171 1393.6196
3949 697.8171 1393.6196
3950 697.8171 1393.6196
3951 697.8171 1393.6196
3952 697.8171 1393.6196
3953 697.8171 1393.6196
3954 697.8171 1393.6196
3957 697.8171 1393.6196
3958 697.8171 1393.6196
3959 697.8171 1393.6196
3960 697.8171 1393.6196
3961 697.8171 1393.6196
3962 697.8171 1393.6196
3963 697.8171 1393.6196
3964 697.8171 1393.6196
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