MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4401–4500 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3712 670.8048 1339.5951
3713 670.8048 1339.5951
3714 670.8048 1339.5951
3715 670.8048 1339.5951
3716 670.8048 1339.5951
3717 670.8048 1339.5951
3718 670.8048 1339.5951
3719 670.8048 1339.5951
3720 670.8048 1339.5951
3721 670.8048 1339.5951
3722 670.8048 1339.5951
3723 670.8048 1339.5951
3724 670.8048 1339.5951
3725 670.8048 1339.5951
3726 670.8048 1339.5951
3727 670.8048 1339.5951
3728 670.8048 1339.5951
3729 670.8048 1339.5951
3730 670.8048 1339.5951
3731 670.8048 1339.5951
3732 670.8048 1339.5951
3733 670.8048 1339.5951
3734 670.8048 1339.5951
3735 684.3109 1366.6073
3736 684.3109 1366.6073
3737 684.3109 1366.6073
3738 684.3109 1366.6073
3739 684.3109 1366.6073
3740 684.3109 1366.6073
3741 684.3109 1366.6073
3742 684.3109 1366.6073
3743 684.3109 1366.6073
3744 684.3109 1366.6073
3745 684.3109 1366.6073
3746 684.3109 1366.6073
3747 684.3109 1366.6073
3748 684.3109 1366.6073
3749 684.3109 1366.6073
3750 684.3109 1366.6073
3751 684.3109 1366.6073
3753 684.3109 1366.6073
3756 684.3109 1366.6073
3760 684.3109 1366.6073
3762 684.3109 1366.6073
3765 684.3109 1366.6073
3766 684.3109 1366.6073
3768 684.3109 1366.6073
3770 684.3109 1366.6073
3771 684.3109 1366.6073
3772 684.3109 1366.6073
3773 684.3109 1366.6073
3774 684.3109 1366.6073
3775 684.3109 1366.6073
3778 684.3109 1366.6073
3779 684.3109 1366.6073
3780 684.3109 1366.6073
3781 684.3109 1366.6073
3782 684.3109 1366.6073
3783 684.3109 1366.6073
3784 684.3109 1366.6073
3785 684.3109 1366.6073
3786 684.3109 1366.6073
3787 684.3109 1366.6073
3788 684.3109 1366.6073
3789 684.3109 1366.6073
3790 684.3109 1366.6073
3791 684.3109 1366.6073
3792 684.3109 1366.6073
3793 684.3109 1366.6073
3794 684.3109 1366.6073
3798 684.3109 1366.6073
3799 684.3109 1366.6073
3800 684.3109 1366.6073
3801 684.3109 1366.6073
3802 684.3109 1366.6073
3804 684.3109 1366.6073
3806 684.3109 1366.6073
3808 684.3109 1366.6073
3809 684.3109 1366.6073
3810 684.3109 1366.6073
3811 684.3109 1366.6073
3812 684.3109 1366.6073
3813 684.3109 1366.6073
3815 684.3109 1366.6073
3818 684.3109 1366.6073
3819 684.3109 1366.6073
3821 684.3109 1366.6073
3822 684.3109 1366.6073
3823 684.3109 1366.6073
3825 684.3109 1366.6073
3826 684.3109 1366.6073
3829 684.3109 1366.6073
3830 684.3109 1366.6073
3831 684.3109 1366.6073
3832 684.3109 1366.6073
3833 684.3109 1366.6073
3834 684.3109 1366.6073
3837 684.3109 1366.6073
3838 684.3109 1366.6073
3839 684.3109 1366.6073
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