MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4201–4300 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3461 657.2986 1312.5827
3462 657.2986 1312.5827
3463 657.2986 1312.5827
3464 657.2986 1312.5827
3465 657.2986 1312.5827
3466 657.2986 1312.5827
3468 657.2986 1312.5827
3470 657.2986 1312.5827
3474 657.2986 1312.5827
3475 657.2986 1312.5827
3479 657.2986 1312.5827
3480 657.2986 1312.5827
3485 657.2986 1312.5827
3486 657.2986 1312.5827
3487 657.2986 1312.5827
3488 657.2986 1312.5827
3490 657.2986 1312.5827
3491 657.2986 1312.5827
3493 657.2986 1312.5827
3494 657.2986 1312.5827
3495 657.2986 1312.5827
3496 657.2986 1312.5827
3497 657.2986 1312.5827
3498 657.2986 1312.5827
3499 657.2986 1312.5827
3501 657.2986 1312.5827
3502 657.2986 1312.5827
3503 657.2986 1312.5827
3504 657.2986 1312.5827
3505 657.2986 1312.5827
3506 657.2986 1312.5827
3507 657.2986 1312.5827
3508 657.2986 1312.5827
3509 657.2986 1312.5827
3510 657.2986 1312.5827
3511 657.2986 1312.5827
3512 657.2986 1312.5827
3513 657.2986 1312.5827
3514 657.2986 1312.5827
3516 657.2986 1312.5827
3519 657.2986 1312.5827
3521 657.2986 1312.5827
3522 657.2986 1312.5827
3523 657.2986 1312.5827
3525 657.2986 1312.5827
3526 657.2986 1312.5827
3528 657.2986 1312.5827
3529 657.2986 1312.5827
3530 657.2986 1312.5827
3531 657.2986 1312.5827
3532 657.2986 1312.5827
3533 657.2986 1312.5827
3534 657.2986 1312.5827
3535 657.2986 1312.5827
3536 657.2986 1312.5827
3537 657.2986 1312.5827
3538 657.2986 1312.5827
3539 657.2986 1312.5827
3540 657.2986 1312.5827
3541 657.2986 1312.5827
3542 657.2986 1312.5827
3543 657.2986 1312.5827
3544 657.2986 1312.5827
3545 657.2986 1312.5827
3546 657.2986 1312.5827
3547 657.2986 1312.5827
3548 657.2986 1312.5827
3549 657.2986 1312.5827
3550 657.2986 1312.5827
3551 657.2986 1312.5827
3552 657.2986 1312.5827
3555 657.2986 1312.5827
3556 657.2986 1312.5827
3557 657.2986 1312.5827
3558 657.2986 1312.5827
3560 657.2986 1312.5827
3561 657.2986 1312.5827
3562 657.2986 1312.5827
3563 657.2986 1312.5827
3564 657.2986 1312.5827
3565 657.2986 1312.5827
3566 670.8048 1339.5951
3567 670.8048 1339.5951
3568 670.8048 1339.5951
3569 670.8048 1339.5951
3570 670.8048 1339.5951
3571 670.8048 1339.5951
3572 670.8048 1339.5951
3573 670.8048 1339.5951
3574 670.8048 1339.5951
3575 670.8048 1339.5951
3576 670.8048 1339.5951
3577 670.8048 1339.5951
3578 670.8048 1339.5951
3580 670.8048 1339.5951
3581 670.8048 1339.5951
3582 670.8048 1339.5951
3583 670.8048 1339.5951
3584 670.8048 1339.5951
3586 670.8048 1339.5951
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