MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4101–4200 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3319 643.7925 1285.5705
3321 643.7925 1285.5705
3322 643.7925 1285.5705
3324 643.7925 1285.5705
3325 643.7925 1285.5705
3327 643.7925 1285.5705
3328 643.7925 1285.5705
3329 643.7925 1285.5705
3330 643.7925 1285.5705
3331 643.7925 1285.5705
3332 643.7925 1285.5705
3333 643.7925 1285.5705
3334 643.7925 1285.5705
3335 643.7925 1285.5705
3336 643.7925 1285.5705
3337 643.7925 1285.5705
3338 643.7925 1285.5705
3339 643.7925 1285.5705
3340 643.7925 1285.5705
3341 643.7925 1285.5705
3342 643.7925 1285.5705
3343 643.7925 1285.5705
3344 643.7925 1285.5705
3345 643.7925 1285.5705
3346 643.7925 1285.5705
3347 643.7925 1285.5705
3348 643.7925 1285.5705
3350 643.7925 1285.5705
3351 643.7925 1285.5705
3353 643.7925 1285.5705
3354 643.7925 1285.5705
3356 643.7925 1285.5705
3358 643.7925 1285.5705
3360 643.7925 1285.5705
3362 643.7925 1285.5705
3363 643.7925 1285.5705
3365 643.7925 1285.5705
3366 643.7925 1285.5705
3367 643.7925 1285.5705
3368 643.7925 1285.5705
3369 643.7925 1285.5705
3370 643.7925 1285.5705
3371 643.7925 1285.5705
3372 643.7925 1285.5705
3373 643.7925 1285.5705
3374 643.7925 1285.5705
3375 643.7925 1285.5705
3379 643.7925 1285.5705
3380 643.7925 1285.5705
3382 643.7925 1285.5705
3383 643.7925 1285.5705
3384 643.7925 1285.5705
3385 643.7925 1285.5705
3386 643.7925 1285.5705
3387 643.7925 1285.5705
3388 643.7925 1285.5705
3389 643.7925 1285.5705
3391 643.7925 1285.5705
3392 643.7925 1285.5705
3393 643.7925 1285.5705
3394 643.7925 1285.5705
3395 643.7925 1285.5705
3396 643.7925 1285.5705
3397 657.2986 1312.5827
3398 657.2986 1312.5827
3399 657.2986 1312.5827
3400 657.2986 1312.5827
3401 657.2986 1312.5827
3402 657.2986 1312.5827
3403 657.2986 1312.5827
3404 657.2986 1312.5827
3405 657.2986 1312.5827
3406 657.2986 1312.5827
3407 657.2986 1312.5827
3408 657.2986 1312.5827
3409 657.2986 1312.5827
3410 657.2986 1312.5827
3411 657.2986 1312.5827
3412 657.2986 1312.5827
3414 657.2986 1312.5827
3415 657.2986 1312.5827
3420 657.2986 1312.5827
3422 657.2986 1312.5827
3423 657.2986 1312.5827
3426 657.2986 1312.5827
3428 657.2986 1312.5827
3430 657.2986 1312.5827
3431 657.2986 1312.5827
3433 657.2986 1312.5827
3437 657.2986 1312.5827
3442 657.2986 1312.5827
3446 657.2986 1312.5827
3447 657.2986 1312.5827
3450 657.2986 1312.5827
3451 657.2986 1312.5827
3454 657.2986 1312.5827
3455 657.2986 1312.5827
3458 657.2986 1312.5827
3459 657.2986 1312.5827
3460 657.2986 1312.5827
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