MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4001–4100 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3192 630.2864 1258.5582
3193 630.2864 1258.5582
3194 630.2864 1258.5582
3195 630.2864 1258.5582
3196 630.2864 1258.5582
3198 630.2864 1258.5582
3199 630.2864 1258.5582
3200 630.2864 1258.5582
3201 630.2864 1258.5582
3202 630.2864 1258.5582
3203 630.2864 1258.5582
3204 630.2864 1258.5582
3205 630.2864 1258.5582
3207 630.2864 1258.5582
3208 630.2864 1258.5582
3209 630.2864 1258.5582
3210 630.2864 1258.5582
3212 630.2864 1258.5582
3213 630.2864 1258.5582
3214 630.2864 1258.5582
3215 630.2864 1258.5582
3216 630.2864 1258.5582
3217 630.2864 1258.5582
3218 630.2864 1258.5582
3219 630.2864 1258.5582
3220 630.2864 1258.5582
3221 630.2864 1258.5582
3222 630.2864 1258.5582
3223 630.2864 1258.5582
3224 630.2864 1258.5582
3225 630.2864 1258.5582
3228 643.7925 1285.5705
3229 643.7925 1285.5705
3230 643.7925 1285.5705
3231 643.7925 1285.5705
3232 643.7925 1285.5705
3233 643.7925 1285.5705
3234 643.7925 1285.5705
3235 643.7925 1285.5705
3236 643.7925 1285.5705
3237 643.7925 1285.5705
3238 643.7925 1285.5705
3239 643.7925 1285.5705
3240 643.7925 1285.5705
3241 643.7925 1285.5705
3242 643.7925 1285.5705
3244 643.7925 1285.5705
3245 643.7925 1285.5705
3246 643.7925 1285.5705
3247 643.7925 1285.5705
3248 643.7925 1285.5705
3250 643.7925 1285.5705
3251 643.7925 1285.5705
3252 643.7925 1285.5705
3253 643.7925 1285.5705
3257 643.7925 1285.5705
3258 643.7925 1285.5705
3259 643.7925 1285.5705
3261 643.7925 1285.5705
3262 643.7925 1285.5705
3264 643.7925 1285.5705
3266 643.7925 1285.5705
3269 643.7925 1285.5705
3272 643.7925 1285.5705
3275 643.7925 1285.5705
3276 643.7925 1285.5705
3277 643.7925 1285.5705
3278 643.7925 1285.5705
3279 643.7925 1285.5705
3281 643.7925 1285.5705
3282 643.7925 1285.5705
3283 643.7925 1285.5705
3284 643.7925 1285.5705
3286 643.7925 1285.5705
3287 643.7925 1285.5705
3288 643.7925 1285.5705
3289 643.7925 1285.5705
3290 643.7925 1285.5705
3293 643.7925 1285.5705
3294 643.7925 1285.5705
3295 643.7925 1285.5705
3296 643.7925 1285.5705
3297 643.7925 1285.5705
3298 643.7925 1285.5705
3300 643.7925 1285.5705
3301 643.7925 1285.5705
3303 643.7925 1285.5705
3304 643.7925 1285.5705
3305 643.7925 1285.5705
3307 643.7925 1285.5705
3308 643.7925 1285.5705
3309 643.7925 1285.5705
3311 643.7925 1285.5705
3312 643.7925 1285.5705
3313 643.7925 1285.5705
3314 643.7925 1285.5705
3315 643.7925 1285.5705
3316 643.7925 1285.5705
3317 643.7925 1285.5705
3318 643.7925 1285.5705
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