MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3901–4000 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3057 616.7802 1231.5459
3058 616.7802 1231.5459
3059 630.2864 1258.5582
3060 630.2864 1258.5582
3061 630.2864 1258.5582
3062 630.2864 1258.5582
3063 630.2864 1258.5582
3064 630.2864 1258.5582
3065 630.2864 1258.5582
3066 630.2864 1258.5582
3067 630.2864 1258.5582
3068 630.2864 1258.5582
3069 630.2864 1258.5582
3070 630.2864 1258.5582
3071 630.2864 1258.5582
3072 630.2864 1258.5582
3073 630.2864 1258.5582
3074 630.2864 1258.5582
3075 630.2864 1258.5582
3076 630.2864 1258.5582
3077 630.2864 1258.5582
3083 630.2864 1258.5582
3084 630.2864 1258.5582
3085 630.2864 1258.5582
3088 630.2864 1258.5582
3089 630.2864 1258.5582
3092 630.2864 1258.5582
3093 630.2864 1258.5582
3095 630.2864 1258.5582
3096 630.2864 1258.5582
3098 630.2864 1258.5582
3100 630.2864 1258.5582
3102 630.2864 1258.5582
3103 630.2864 1258.5582
3105 630.2864 1258.5582
3108 630.2864 1258.5582
3109 630.2864 1258.5582
3110 630.2864 1258.5582
3111 630.2864 1258.5582
3112 630.2864 1258.5582
3113 630.2864 1258.5582
3114 630.2864 1258.5582
3115 630.2864 1258.5582
3116 630.2864 1258.5582
3118 630.2864 1258.5582
3120 630.2864 1258.5582
3122 630.2864 1258.5582
3124 630.2864 1258.5582
3125 630.2864 1258.5582
3129 630.2864 1258.5582
3131 630.2864 1258.5582
3132 630.2864 1258.5582
3133 630.2864 1258.5582
3134 630.2864 1258.5582
3136 630.2864 1258.5582
3137 630.2864 1258.5582
3138 630.2864 1258.5582
3141 630.2864 1258.5582
3142 630.2864 1258.5582
3143 630.2864 1258.5582
3144 630.2864 1258.5582
3145 630.2864 1258.5582
3146 630.2864 1258.5582
3147 630.2864 1258.5582
3148 630.2864 1258.5582
3151 630.2864 1258.5582
3152 630.2864 1258.5582
3153 630.2864 1258.5582
3154 630.2864 1258.5582
3155 630.2864 1258.5582
3156 630.2864 1258.5582
3157 630.2864 1258.5582
3158 630.2864 1258.5582
3159 630.2864 1258.5582
3160 630.2864 1258.5582
3161 630.2864 1258.5582
3162 630.2864 1258.5582
3163 630.2864 1258.5582
3164 630.2864 1258.5582
3165 630.2864 1258.5582
3166 630.2864 1258.5582
3167 630.2864 1258.5582
3171 630.2864 1258.5582
3172 630.2864 1258.5582
3173 630.2864 1258.5582
3174 630.2864 1258.5582
3175 630.2864 1258.5582
3176 630.2864 1258.5582
3177 630.2864 1258.5582
3178 630.2864 1258.5582
3180 630.2864 1258.5582
3181 630.2864 1258.5582
3182 630.2864 1258.5582
3183 630.2864 1258.5582
3184 630.2864 1258.5582
3186 630.2864 1258.5582
3188 630.2864 1258.5582
3189 630.2864 1258.5582
3190 630.2864 1258.5582
3191 630.2864 1258.5582
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