MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3801–3900 (out of 7566)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2936 616.7802 1231.5459
2937 616.7802 1231.5459
2938 616.7802 1231.5459
2939 616.7802 1231.5459
2942 616.7802 1231.5459
2943 616.7802 1231.5459
2944 616.7802 1231.5459
2946 616.7802 1231.5459
2947 616.7802 1231.5459
2949 616.7802 1231.5459
2950 616.7802 1231.5459
2951 616.7802 1231.5459
2952 616.7802 1231.5459
2953 616.7802 1231.5459
2954 616.7802 1231.5459
2955 616.7802 1231.5459
2956 616.7802 1231.5459
2957 616.7802 1231.5459
2958 616.7802 1231.5459
2959 616.7802 1231.5459
2960 616.7802 1231.5459
2961 616.7802 1231.5459
2962 616.7802 1231.5459
2963 616.7802 1231.5459
2964 616.7802 1231.5459
2965 616.7802 1231.5459
2966 616.7802 1231.5459
2967 616.7802 1231.5459
2968 616.7802 1231.5459
2969 616.7802 1231.5459
2971 616.7802 1231.5459
2972 616.7802 1231.5459
2974 616.7802 1231.5459
2975 616.7802 1231.5459
2976 616.7802 1231.5459
2977 616.7802 1231.5459
2978 616.7802 1231.5459
2979 616.7802 1231.5459
2980 616.7802 1231.5459
2981 616.7802 1231.5459
2982 616.7802 1231.5459
2983 616.7802 1231.5459
2986 616.7802 1231.5459
2987 616.7802 1231.5459
2988 616.7802 1231.5459
2989 616.7802 1231.5459
2991 616.7802 1231.5459
2992 616.7802 1231.5459
2993 616.7802 1231.5459
2994 616.7802 1231.5459
2995 616.7802 1231.5459
2996 616.7802 1231.5459
2997 616.7802 1231.5459
2998 616.7802 1231.5459
2999 616.7802 1231.5459
3000 616.7802 1231.5459
3001 616.7802 1231.5459
3002 616.7802 1231.5459
3003 616.7802 1231.5459
3004 616.7802 1231.5459
3005 616.7802 1231.5459
3007 616.7802 1231.5459
3008 616.7802 1231.5459
3009 616.7802 1231.5459
3010 616.7802 1231.5459
3011 616.7802 1231.5459
3012 616.7802 1231.5459
3015 616.7802 1231.5459
3016 616.7802 1231.5459
3017 616.7802 1231.5459
3019 616.7802 1231.5459
3021 616.7802 1231.5459
3024 616.7802 1231.5459
3025 616.7802 1231.5459
3026 616.7802 1231.5459
3027 616.7802 1231.5459
3029 616.7802 1231.5459
3031 616.7802 1231.5459
3033 616.7802 1231.5459
3034 616.7802 1231.5459
3035 616.7802 1231.5459
3036 616.7802 1231.5459
3037 616.7802 1231.5459
3038 616.7802 1231.5459
3039 616.7802 1231.5459
3041 616.7802 1231.5459
3042 616.7802 1231.5459
3043 616.7802 1231.5459
3045 616.7802 1231.5459
3046 616.7802 1231.5459
3047 616.7802 1231.5459
3048 616.7802 1231.5459
3049 616.7802 1231.5459
3050 616.7802 1231.5459
3051 616.7802 1231.5459
3052 616.7802 1231.5459
3053 616.7802 1231.5459
3054 616.7802 1231.5459
3055 616.7802 1231.5459
3056 616.7802 1231.5459
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