MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3701–3800 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2803 603.2741 1204.5337
2804 603.2741 1204.5337
2806 603.2741 1204.5337
2807 603.2741 1204.5337
2809 603.2741 1204.5337
2810 603.2741 1204.5337
2811 603.2741 1204.5337
2812 603.2741 1204.5337
2813 603.2741 1204.5337
2814 603.2741 1204.5337
2815 603.2741 1204.5337
2816 603.2741 1204.5337
2817 603.2741 1204.5337
2818 603.2741 1204.5337
2819 603.2741 1204.5337
2821 603.2741 1204.5337
2823 603.2741 1204.5337
2824 603.2741 1204.5337
2825 603.2741 1204.5337
2827 603.2741 1204.5337
2829 603.2741 1204.5337
2831 603.2741 1204.5337
2832 603.2741 1204.5337
2833 603.2741 1204.5337
2834 603.2741 1204.5337
2835 603.2741 1204.5337
2837 603.2741 1204.5337
2840 603.2741 1204.5337
2841 603.2741 1204.5337
2843 603.2741 1204.5337
2844 603.2741 1204.5337
2848 603.2741 1204.5337
2849 603.2741 1204.5337
2852 603.2741 1204.5337
2853 603.2741 1204.5337
2854 603.2741 1204.5337
2855 603.2741 1204.5337
2857 603.2741 1204.5337
2858 603.2741 1204.5337
2859 603.2741 1204.5337
2860 603.2741 1204.5337
2861 603.2741 1204.5337
2864 603.2741 1204.5337
2866 603.2741 1204.5337
2867 603.2741 1204.5337
2868 603.2741 1204.5337
2869 603.2741 1204.5337
2870 603.2741 1204.5337
2871 603.2741 1204.5337
2872 603.2741 1204.5337
2873 603.2741 1204.5337
2875 603.2741 1204.5337
2877 603.2741 1204.5337
2878 603.2741 1204.5337
2880 603.2741 1204.5337
2881 603.2741 1204.5337
2882 603.2741 1204.5337
2883 603.2741 1204.5337
2884 603.2741 1204.5337
2885 603.2741 1204.5337
2886 603.2741 1204.5337
2887 603.2741 1204.5337
2888 603.2741 1204.5337
2889 603.2741 1204.5337
2890 616.7802 1231.5459
2891 616.7802 1231.5459
2892 616.7802 1231.5459
2893 616.7802 1231.5459
2894 616.7802 1231.5459
2895 616.7802 1231.5459
2896 616.7802 1231.5459
2897 616.7802 1231.5459
2898 616.7802 1231.5459
2899 616.7802 1231.5459
2900 616.7802 1231.5459
2901 616.7802 1231.5459
2903 616.7802 1231.5459
2904 616.7802 1231.5459
2908 616.7802 1231.5459
2909 616.7802 1231.5459
2911 616.7802 1231.5459
2912 616.7802 1231.5459
2913 616.7802 1231.5459
2914 616.7802 1231.5459
2916 616.7802 1231.5459
2917 616.7802 1231.5459
2919 616.7802 1231.5459
2920 616.7802 1231.5459
2921 616.7802 1231.5459
2922 616.7802 1231.5459
2923 616.7802 1231.5459
2924 616.7802 1231.5459
2925 616.7802 1231.5459
2927 616.7802 1231.5459
2928 616.7802 1231.5459
2930 616.7802 1231.5459
2931 616.7802 1231.5459
2932 616.7802 1231.5459
2933 616.7802 1231.5459
2935 616.7802 1231.5459
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