MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3601–3700 (out of 7566)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2658 589.7679 1177.5213
2659 589.7679 1177.5213
2660 589.7679 1177.5213
2661 589.7679 1177.5213
2662 589.7679 1177.5213
2663 589.7679 1177.5213
2664 589.7679 1177.5213
2665 589.7679 1177.5213
2666 589.7679 1177.5213
2669 589.7679 1177.5213
2670 589.7679 1177.5213
2671 589.7679 1177.5213
2672 589.7679 1177.5213
2673 589.7679 1177.5213
2674 589.7679 1177.5213
2675 589.7679 1177.5213
2676 589.7679 1177.5213
2677 589.7679 1177.5213
2678 589.7679 1177.5213
2679 589.7679 1177.5213
2685 589.7679 1177.5213
2686 589.7679 1177.5213
2688 589.7679 1177.5213
2689 589.7679 1177.5213
2690 589.7679 1177.5213
2691 589.7679 1177.5213
2692 589.7679 1177.5213
2694 589.7679 1177.5213
2697 589.7679 1177.5213
2698 589.7679 1177.5213
2699 589.7679 1177.5213
2700 589.7679 1177.5213
2701 589.7679 1177.5213
2703 589.7679 1177.5213
2706 589.7679 1177.5213
2708 589.7679 1177.5213
2709 589.7679 1177.5213
2711 589.7679 1177.5213
2712 589.7679 1177.5213
2713 589.7679 1177.5213
2714 589.7679 1177.5213
2716 589.7679 1177.5213
2717 589.7679 1177.5213
2718 589.7679 1177.5213
2719 589.7679 1177.5213
2720 589.7679 1177.5213
2721 603.2741 1204.5337
2722 603.2741 1204.5337
2723 603.2741 1204.5337
2724 603.2741 1204.5337
2725 603.2741 1204.5337
2726 603.2741 1204.5337
2728 603.2741 1204.5337
2729 603.2741 1204.5337
2730 603.2741 1204.5337
2731 603.2741 1204.5337
2732 603.2741 1204.5337
2733 603.2741 1204.5337
2734 603.2741 1204.5337
2735 603.2741 1204.5337
2736 603.2741 1204.5337
2737 603.2741 1204.5337
2738 603.2741 1204.5337
2739 603.2741 1204.5337
2741 603.2741 1204.5337
2743 603.2741 1204.5337
2744 603.2741 1204.5337
2745 603.2741 1204.5337
2746 603.2741 1204.5337
2747 603.2741 1204.5337
2750 603.2741 1204.5337
2752 603.2741 1204.5337
2753 603.2741 1204.5337
2754 603.2741 1204.5337
2756 603.2741 1204.5337
2757 603.2741 1204.5337
2759 603.2741 1204.5337
2760 603.2741 1204.5337
2761 603.2741 1204.5337
2762 603.2741 1204.5337
2763 603.2741 1204.5337
2764 603.2741 1204.5337
2766 603.2741 1204.5337
2767 603.2741 1204.5337
2769 603.2741 1204.5337
2772 603.2741 1204.5337
2775 603.2741 1204.5337
2779 603.2741 1204.5337
2783 603.2741 1204.5337
2787 603.2741 1204.5337
2788 603.2741 1204.5337
2790 603.2741 1204.5337
2792 603.2741 1204.5337
2794 603.2741 1204.5337
2795 603.2741 1204.5337
2796 603.2741 1204.5337
2798 603.2741 1204.5337
2799 603.2741 1204.5337
2800 603.2741 1204.5337
2801 603.2741 1204.5337
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