MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3501–3600 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2519 576.2618 1150.5091
2522 576.2618 1150.5091
2526 576.2618 1150.5091
2528 576.2618 1150.5091
2529 576.2618 1150.5091
2530 576.2618 1150.5091
2531 576.2618 1150.5091
2532 576.2618 1150.5091
2534 576.2618 1150.5091
2535 576.2618 1150.5091
2537 576.2618 1150.5091
2538 576.2618 1150.5091
2539 576.2618 1150.5091
2540 576.2618 1150.5091
2542 576.2618 1150.5091
2543 576.2618 1150.5091
2544 576.2618 1150.5091
2545 576.2618 1150.5091
2546 576.2618 1150.5091
2547 576.2618 1150.5091
2548 576.2618 1150.5091
2549 576.2618 1150.5091
2550 576.2618 1150.5091
2551 589.7679 1177.5213
2552 589.7679 1177.5213
2553 589.7679 1177.5213
2554 589.7679 1177.5213
2555 589.7679 1177.5213
2556 589.7679 1177.5213
2557 589.7679 1177.5213
2558 589.7679 1177.5213
2559 589.7679 1177.5213
2560 589.7679 1177.5213
2561 589.7679 1177.5213
2562 589.7679 1177.5213
2563 589.7679 1177.5213
2564 589.7679 1177.5213
2565 589.7679 1177.5213
2567 589.7679 1177.5213
2570 589.7679 1177.5213
2572 589.7679 1177.5213
2573 589.7679 1177.5213
2574 589.7679 1177.5213
2577 589.7679 1177.5213
2580 589.7679 1177.5213
2582 589.7679 1177.5213
2583 589.7679 1177.5213
2584 589.7679 1177.5213
2585 589.7679 1177.5213
2586 589.7679 1177.5213
2587 589.7679 1177.5213
2588 589.7679 1177.5213
2590 589.7679 1177.5213
2591 589.7679 1177.5213
2592 589.7679 1177.5213
2596 589.7679 1177.5213
2597 589.7679 1177.5213
2598 589.7679 1177.5213
2599 589.7679 1177.5213
2600 589.7679 1177.5213
2601 589.7679 1177.5213
2604 589.7679 1177.5213
2605 589.7679 1177.5213
2606 589.7679 1177.5213
2608 589.7679 1177.5213
2609 589.7679 1177.5213
2611 589.7679 1177.5213
2612 589.7679 1177.5213
2613 589.7679 1177.5213
2614 589.7679 1177.5213
2615 589.7679 1177.5213
2617 589.7679 1177.5213
2618 589.7679 1177.5213
2620 589.7679 1177.5213
2621 589.7679 1177.5213
2622 589.7679 1177.5213
2625 589.7679 1177.5213
2627 589.7679 1177.5213
2629 589.7679 1177.5213
2630 589.7679 1177.5213
2631 589.7679 1177.5213
2634 589.7679 1177.5213
2635 589.7679 1177.5213
2636 589.7679 1177.5213
2637 589.7679 1177.5213
2639 589.7679 1177.5213
2640 589.7679 1177.5213
2642 589.7679 1177.5213
2644 589.7679 1177.5213
2645 589.7679 1177.5213
2646 589.7679 1177.5213
2647 589.7679 1177.5213
2649 589.7679 1177.5213
2650 589.7679 1177.5213
2651 589.7679 1177.5213
2652 589.7679 1177.5213
2653 589.7679 1177.5213
2655 589.7679 1177.5213
2656 589.7679 1177.5213
2657 589.7679 1177.5213
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