MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3301–3400 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2240 562.7557 1123.4968
2242 562.7557 1123.4968
2245 562.7557 1123.4968
2250 562.7557 1123.4968
2251 562.7557 1123.4968
2252 562.7557 1123.4968
2257 562.7557 1123.4968
2258 562.7557 1123.4968
2261 562.7557 1123.4968
2262 562.7557 1123.4968
2265 562.7557 1123.4968
2267 562.7557 1123.4968
2268 562.7557 1123.4968
2269 562.7557 1123.4968
2270 562.7557 1123.4968
2271 562.7557 1123.4968
2272 562.7557 1123.4968
2273 562.7557 1123.4968
2274 562.7557 1123.4968
2277 562.7557 1123.4968
2278 562.7557 1123.4968
2279 562.7557 1123.4968
2281 562.7557 1123.4968
2282 562.7557 1123.4968
2284 562.7557 1123.4968
2285 562.7557 1123.4968
2287 562.7557 1123.4968
2288 562.7557 1123.4968
2289 562.7557 1123.4968
2290 562.7557 1123.4968
2292 562.7557 1123.4968
2293 562.7557 1123.4968
2294 562.7557 1123.4968
2296 562.7557 1123.4968
2298 562.7557 1123.4968
2300 562.7557 1123.4968
2301 562.7557 1123.4968
2302 562.7557 1123.4968
2304 562.7557 1123.4968
2305 562.7557 1123.4968
2306 562.7557 1123.4968
2308 562.7557 1123.4968
2310 562.7557 1123.4968
2311 562.7557 1123.4968
2312 562.7557 1123.4968
2313 562.7557 1123.4968
2314 562.7557 1123.4968
2315 562.7557 1123.4968
2317 562.7557 1123.4968
2318 562.7557 1123.4968
2321 562.7557 1123.4968
2322 562.7557 1123.4968
2323 562.7557 1123.4968
2325 562.7557 1123.4968
2326 562.7557 1123.4968
2327 562.7557 1123.4968
2328 562.7557 1123.4968
2329 562.7557 1123.4968
2330 562.7557 1123.4968
2331 562.7557 1123.4968
2333 562.7557 1123.4968
2334 562.7557 1123.4968
2335 562.7557 1123.4968
2337 562.7557 1123.4968
2338 562.7557 1123.4968
2339 562.7557 1123.4968
2340 562.7557 1123.4968
2341 562.7557 1123.4968
2342 562.7557 1123.4968
2343 562.7557 1123.4968
2344 562.7557 1123.4968
2346 562.7557 1123.4968
2347 562.7557 1123.4968
2349 562.7557 1123.4968
2350 562.7557 1123.4968
2351 562.7557 1123.4968
2352 562.7557 1123.4968
2353 562.7557 1123.4968
2354 562.7557 1123.4968
2356 562.7557 1123.4968
2360 562.7557 1123.4968
2361 562.7557 1123.4968
2362 562.7557 1123.4968
2363 562.7557 1123.4968
2364 562.7557 1123.4968
2365 562.7557 1123.4968
2366 562.7557 1123.4968
2367 562.7557 1123.4968
2369 562.7557 1123.4968
2371 562.7557 1123.4968
2373 562.7557 1123.4968
2374 562.7557 1123.4968
2375 562.7557 1123.4968
2376 562.7557 1123.4968
2377 562.7557 1123.4968
2378 562.7557 1123.4968
2379 562.7557 1123.4968
2380 562.7557 1123.4968
2381 576.2618 1150.5091
2382 576.2618 1150.5091
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