MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3201–3300 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2093 549.2496 1096.4846
2094 549.2496 1096.4846
2103 549.2496 1096.4846
2104 549.2496 1096.4846
2106 549.2496 1096.4846
2107 549.2496 1096.4846
2109 549.2496 1096.4846
2110 549.2496 1096.4846
2112 549.2496 1096.4846
2114 549.2496 1096.4846
2115 549.2496 1096.4846
2116 549.2496 1096.4846
2118 549.2496 1096.4846
2123 549.2496 1096.4846
2124 549.2496 1096.4846
2125 549.2496 1096.4846
2126 549.2496 1096.4846
2128 549.2496 1096.4846
2129 549.2496 1096.4846
2130 549.2496 1096.4846
2131 549.2496 1096.4846
2135 549.2496 1096.4846
2136 549.2496 1096.4846
2137 549.2496 1096.4846
2138 549.2496 1096.4846
2139 549.2496 1096.4846
2140 549.2496 1096.4846
2141 549.2496 1096.4846
2142 549.2496 1096.4846
2143 549.2496 1096.4846
2144 549.2496 1096.4846
2145 549.2496 1096.4846
2146 549.2496 1096.4846
2147 549.2496 1096.4846
2149 549.2496 1096.4846
2150 549.2496 1096.4846
2152 549.2496 1096.4846
2153 549.2496 1096.4846
2155 549.2496 1096.4846
2156 549.2496 1096.4846
2157 549.2496 1096.4846
2158 549.2496 1096.4846
2160 549.2496 1096.4846
2161 549.2496 1096.4846
2162 549.2496 1096.4846
2163 549.2496 1096.4846
2164 549.2496 1096.4846
2165 549.2496 1096.4846
2166 549.2496 1096.4846
2167 549.2496 1096.4846
2168 549.2496 1096.4846
2170 549.2496 1096.4846
2171 549.2496 1096.4846
2174 549.2496 1096.4846
2175 549.2496 1096.4846
2176 549.2496 1096.4846
2177 549.2496 1096.4846
2179 549.2496 1096.4846
2180 549.2496 1096.4846
2181 549.2496 1096.4846
2182 549.2496 1096.4846
2185 549.2496 1096.4846
2186 549.2496 1096.4846
2187 549.2496 1096.4846
2188 549.2496 1096.4846
2189 549.2496 1096.4846
2190 549.2496 1096.4846
2193 549.2496 1096.4846
2195 549.2496 1096.4846
2196 549.2496 1096.4846
2197 549.2496 1096.4846
2200 549.2496 1096.4846
2201 549.2496 1096.4846
2202 549.2496 1096.4846
2203 549.2496 1096.4846
2205 549.2496 1096.4846
2206 549.2496 1096.4846
2207 549.2496 1096.4846
2208 549.2496 1096.4846
2209 549.2496 1096.4846
2210 549.2496 1096.4846
2211 562.7557 1123.4968
2212 562.7557 1123.4968
2213 562.7557 1123.4968
2214 562.7557 1123.4968
2215 562.7557 1123.4968
2216 562.7557 1123.4968
2217 562.7557 1123.4968
2218 562.7557 1123.4968
2219 562.7557 1123.4968
2220 562.7557 1123.4968
2222 562.7557 1123.4968
2223 562.7557 1123.4968
2224 562.7557 1123.4968
2227 562.7557 1123.4968
2229 562.7557 1123.4968
2233 562.7557 1123.4968
2235 562.7557 1123.4968
2237 562.7557 1123.4968
2238 562.7557 1123.4968
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