MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3101–3200 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1966 535.7434 1069.4723
1967 535.7434 1069.4723
1968 535.7434 1069.4723
1969 535.7434 1069.4723
1970 535.7434 1069.4723
1972 535.7434 1069.4723
1974 535.7434 1069.4723
1975 535.7434 1069.4723
1976 535.7434 1069.4723
1977 535.7434 1069.4723
1978 535.7434 1069.4723
1979 535.7434 1069.4723
1980 535.7434 1069.4723
1981 535.7434 1069.4723
1982 535.7434 1069.4723
1983 535.7434 1069.4723
1984 535.7434 1069.4723
1985 535.7434 1069.4723
1986 535.7434 1069.4723
1987 535.7434 1069.4723
1990 535.7434 1069.4723
1991 535.7434 1069.4723
1992 535.7434 1069.4723
1993 535.7434 1069.4723
1994 535.7434 1069.4723
1995 535.7434 1069.4723
1996 535.7434 1069.4723
1997 535.7434 1069.4723
1998 535.7434 1069.4723
1999 535.7434 1069.4723
2000 535.7434 1069.4723
2001 535.7434 1069.4723
2002 535.7434 1069.4723
2004 535.7434 1069.4723
2005 535.7434 1069.4723
2006 535.7434 1069.4723
2007 535.7434 1069.4723
2008 535.7434 1069.4723
2009 535.7434 1069.4723
2010 535.7434 1069.4723
2011 535.7434 1069.4723
2012 535.7434 1069.4723
2013 535.7434 1069.4723
2016 535.7434 1069.4723
2017 535.7434 1069.4723
2018 535.7434 1069.4723
2021 535.7434 1069.4723
2022 535.7434 1069.4723
2023 535.7434 1069.4723
2024 535.7434 1069.4723
2026 535.7434 1069.4723
2027 535.7434 1069.4723
2028 535.7434 1069.4723
2030 535.7434 1069.4723
2031 535.7434 1069.4723
2032 535.7434 1069.4723
2033 535.7434 1069.4723
2034 535.7434 1069.4723
2036 535.7434 1069.4723
2037 535.7434 1069.4723
2038 535.7434 1069.4723
2039 535.7434 1069.4723
2040 535.7434 1069.4723
2041 549.2496 1096.4846
2042 549.2496 1096.4846
2043 549.2496 1096.4846
2044 549.2496 1096.4846
2045 549.2496 1096.4846
2046 549.2496 1096.4846
2047 549.2496 1096.4846
2048 549.2496 1096.4846
2049 549.2496 1096.4846
2050 549.2496 1096.4846
2051 549.2496 1096.4846
2052 549.2496 1096.4846
2053 549.2496 1096.4846
2054 549.2496 1096.4846
2055 549.2496 1096.4846
2056 549.2496 1096.4846
2057 549.2496 1096.4846
2059 549.2496 1096.4846
2062 549.2496 1096.4846
2063 549.2496 1096.4846
2064 549.2496 1096.4846
2065 549.2496 1096.4846
2066 549.2496 1096.4846
2067 549.2496 1096.4846
2068 549.2496 1096.4846
2070 549.2496 1096.4846
2072 549.2496 1096.4846
2073 549.2496 1096.4846
2074 549.2496 1096.4846
2076 549.2496 1096.4846
2077 549.2496 1096.4846
2082 549.2496 1096.4846
2083 549.2496 1096.4846
2084 549.2496 1096.4846
2085 549.2496 1096.4846
2088 549.2496 1096.4846
2091 549.2496 1096.4846
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