MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3001–3100 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1822 522.2372 1042.4599
1824 522.2372 1042.4599
1827 522.2372 1042.4599
1828 522.2372 1042.4599
1829 522.2372 1042.4599
1831 522.2372 1042.4599
1832 522.2372 1042.4599
1833 522.2372 1042.4599
1837 522.2372 1042.4599
1838 522.2372 1042.4599
1840 522.2372 1042.4599
1841 522.2372 1042.4599
1842 522.2372 1042.4599
1843 522.2372 1042.4599
1844 522.2372 1042.4599
1845 522.2372 1042.4599
1846 522.2372 1042.4599
1847 522.2372 1042.4599
1853 522.2372 1042.4599
1855 522.2372 1042.4599
1856 522.2372 1042.4599
1858 522.2372 1042.4599
1859 522.2372 1042.4599
1860 522.2372 1042.4599
1862 522.2372 1042.4599
1866 522.2372 1042.4599
1867 522.2372 1042.4599
1869 522.2372 1042.4599
1871 535.7434 1069.4723
1872 535.7434 1069.4723
1873 535.7434 1069.4723
1874 535.7434 1069.4723
1875 535.7434 1069.4723
1876 535.7434 1069.4723
1877 535.7434 1069.4723
1878 535.7434 1069.4723
1879 535.7434 1069.4723
1880 535.7434 1069.4723
1881 535.7434 1069.4723
1882 535.7434 1069.4723
1883 535.7434 1069.4723
1884 535.7434 1069.4723
1886 535.7434 1069.4723
1887 535.7434 1069.4723
1888 535.7434 1069.4723
1889 535.7434 1069.4723
1890 535.7434 1069.4723
1891 535.7434 1069.4723
1893 535.7434 1069.4723
1895 535.7434 1069.4723
1896 535.7434 1069.4723
1897 535.7434 1069.4723
1899 535.7434 1069.4723
1900 535.7434 1069.4723
1901 535.7434 1069.4723
1905 535.7434 1069.4723
1907 535.7434 1069.4723
1908 535.7434 1069.4723
1911 535.7434 1069.4723
1912 535.7434 1069.4723
1915 535.7434 1069.4723
1916 535.7434 1069.4723
1920 535.7434 1069.4723
1922 535.7434 1069.4723
1923 535.7434 1069.4723
1924 535.7434 1069.4723
1925 535.7434 1069.4723
1926 535.7434 1069.4723
1927 535.7434 1069.4723
1928 535.7434 1069.4723
1929 535.7434 1069.4723
1931 535.7434 1069.4723
1932 535.7434 1069.4723
1933 535.7434 1069.4723
1934 535.7434 1069.4723
1936 535.7434 1069.4723
1937 535.7434 1069.4723
1938 535.7434 1069.4723
1939 535.7434 1069.4723
1940 535.7434 1069.4723
1941 535.7434 1069.4723
1942 535.7434 1069.4723
1943 535.7434 1069.4723
1944 535.7434 1069.4723
1945 535.7434 1069.4723
1946 535.7434 1069.4723
1947 535.7434 1069.4723
1948 535.7434 1069.4723
1949 535.7434 1069.4723
1951 535.7434 1069.4723
1952 535.7434 1069.4723
1953 535.7434 1069.4723
1954 535.7434 1069.4723
1955 535.7434 1069.4723
1956 535.7434 1069.4723
1957 535.7434 1069.4723
1962 535.7434 1069.4723
1963 535.7434 1069.4723
1964 535.7434 1069.4723
1965 535.7434 1069.4723
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