MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2901–3000 (out of 7566)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1650 508.7311 1015.4477
1651 508.7311 1015.4477
1653 508.7311 1015.4477
1654 508.7311 1015.4477
1655 508.7311 1015.4477
1658 508.7311 1015.4477
1659 508.7311 1015.4477
1660 508.7311 1015.4477
1661 508.7311 1015.4477
1662 508.7311 1015.4477
1663 508.7311 1015.4477
1664 508.7311 1015.4477
1665 508.7311 1015.4477
1666 508.7311 1015.4477
1667 508.7311 1015.4477
1669 508.7311 1015.4477
1670 508.7311 1015.4477
1671 508.7311 1015.4477
1672 508.7311 1015.4477
1673 508.7311 1015.4477
1675 508.7311 1015.4477
1676 508.7311 1015.4477
1677 508.7311 1015.4477
1678 508.7311 1015.4477
1679 508.7311 1015.4477
1683 508.7311 1015.4477
1684 508.7311 1015.4477
1685 508.7311 1015.4477
1686 508.7311 1015.4477
1687 508.7311 1015.4477
1688 508.7311 1015.4477
1689 508.7311 1015.4477
1690 508.7311 1015.4477
1691 508.7311 1015.4477
1693 508.7311 1015.4477
1694 508.7311 1015.4477
1695 508.7311 1015.4477
1696 508.7311 1015.4477
1697 508.7311 1015.4477
1698 508.7311 1015.4477
1699 508.7311 1015.4477
1700 508.7311 1015.4477
1701 522.2372 1042.4599
1702 522.2372 1042.4599
1703 522.2372 1042.4599
1705 522.2372 1042.4599
1706 522.2372 1042.4599
1707 522.2372 1042.4599
1708 522.2372 1042.4599
1709 522.2372 1042.4599
1710 522.2372 1042.4599
1711 522.2372 1042.4599
1712 522.2372 1042.4599
1713 522.2372 1042.4599
1714 522.2372 1042.4599
1715 522.2372 1042.4599
1717 522.2372 1042.4599
1718 522.2372 1042.4599
1719 522.2372 1042.4599
1723 522.2372 1042.4599
1724 522.2372 1042.4599
1726 522.2372 1042.4599
1727 522.2372 1042.4599
1730 522.2372 1042.4599
1731 522.2372 1042.4599
1732 522.2372 1042.4599
1733 522.2372 1042.4599
1735 522.2372 1042.4599
1736 522.2372 1042.4599
1737 522.2372 1042.4599
1739 522.2372 1042.4599
1740 522.2372 1042.4599
1743 522.2372 1042.4599
1744 522.2372 1042.4599
1745 522.2372 1042.4599
1746 522.2372 1042.4599
1748 522.2372 1042.4599
1751 522.2372 1042.4599
1755 522.2372 1042.4599
1758 522.2372 1042.4599
1763 522.2372 1042.4599
1764 522.2372 1042.4599
1772 522.2372 1042.4599
1778 522.2372 1042.4599
1779 522.2372 1042.4599
1784 522.2372 1042.4599
1786 522.2372 1042.4599
1787 522.2372 1042.4599
1791 522.2372 1042.4599
1793 522.2372 1042.4599
1804 522.2372 1042.4599
1805 522.2372 1042.4599
1807 522.2372 1042.4599
1808 522.2372 1042.4599
1810 522.2372 1042.4599
1814 522.2372 1042.4599
1817 522.2372 1042.4599
1819 522.2372 1042.4599
1820 522.2372 1042.4599
1821 522.2372 1042.4599
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