MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 201–300 (out of 7566)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
929 454.7066 1814.7972 1814.7781 10.5 1 8 0.55 +1Score > 17 indicates identity NMTFSKDDWQTQEGK + Deamidated (NQ)
132 387.1759 1158.5058 1158.5267 -18.0 0 8 0.43 +1Score > 16 indicates identity FMAQYQELK + 2 Deamidated (NQ)
5700 832.8785 1663.7424 1663.7487 -3.76 1 8 0.83 +1Score > 19 indicates identity ASETFMYARAMGWK + Oxidation (M)
1127 468.2127 1868.8217 1868.8502 -15.2 0 8 0.32 +1Score > 20 indicates identity
Score > 15 indicates homology
QYGIDAIGFNAPDLNMK + 3 Deamidated (NQ)
1498 495.2250 988.4354 988.4219 13.7 0 8 0.94 +1Score > 20 indicates identity GNHEVMMR + Oxidation (M)
3439 657.2986 1968.8741 1968.8499 12.3 0 7 0.85 +1Score > 21 indicates identity
Score > 19 indicates homology
CFADIQPMTDEFGWPR
778 441.2004 1320.5795 1320.5793 0.14 1 7 0.42 +1Score > 20 indicates identity
Score > 16 indicates homology
ADAEKAEETSNR + Deamidated (NQ)
2345 562.7557 2246.9936 2247.0055 -5.28 1 7 0.68 +1Score > 18 indicates identity YYEPSPGRNYGVGMNIAWR + 2 Deamidated (NQ); Oxidation (M)
451 414.1881 1239.5426 1239.5661 -19.0 0 7 0.86 +1Score > 19 indicates identity SPMELMTMLR + 2 Oxidation (M)
966 454.7066 1814.7972 1814.7849 6.80 0 7 0.58 +1Score > 17 indicates identity MTSSCCVTNNLQASLK + 2 Deamidated (NQ)
2355 562.7557 1123.4968 1123.5179 -18.8 0 7 0.69 +1Score > 18 indicates identity SASLSDIEMR + Oxidation (M)
445 414.1881 1652.7235 1652.7278 -2.62 0 7 0.87 +1Score > 19 indicates identity AYSGEGAIADDAGNVSR + Deamidated (NQ)
6497 900.4092 3597.6076 3597.6790 -19.8 0 7 0.32 +1Score > 20 indicates identity
Score > 15 indicates homology
IDVMLDSDGQFYLLEANTSPGMTSHSLVPMAAR + 2 Oxidation (M)
1384 495.2250 1976.8708 1976.8996 -14.6 0 7 0.31 +1Score > 20 indicates identity
Score > 15 indicates homology
AASEEVINMLSQANPQTR + 3 Deamidated (NQ); Oxidation (M)
837 441.2004 1320.5795 1320.6058 -19.9 1 7 1 +1Score > 20 indicates identity
Score > 20 indicates homology
DNNRVGFAEAAR + 2 Deamidated (NQ)
452 414.1881 1239.5426 1239.5661 -19.0 0 7 0.88 +1Score > 19 indicates identity SPMELMTMLR + 2 Oxidation (M)
1163 468.2127 934.4109 934.4219 -11.8 0 7 0.27 +1Score > 20 indicates identity
Score > 14 indicates homology
TFPDCAPK
2828 603.2741 1204.5337 1204.5394 -4.76 0 7 0.58 +1Score > 20 indicates identity
Score > 17 indicates homology
DDPSMVADLSR
412 414.1881 1239.5426 1239.5661 -19.0 0 7 0.89 +1Score > 19 indicates identity SPMELMTMLR + 2 Oxidation (M)
257 400.6820 1598.6989 1598.7186 -12.3 1 7 0.43 +1Score > 16 indicates identity GGQHSGGNFKNDPQR + Deamidated (NQ)
2520 576.2618 1150.5091 1150.5288 -17.1 0 7 1 +1Score > 20 indicates identity
Score > 20 indicates homology
TGDIMTIGGNR + Deamidated (NQ); Oxidation (M)
2628 589.7679 2355.0427 2355.0280 6.24 1 7 0.83 +1Score > 19 indicates identity GEHHGQKGFFAWFNQMFNR + 2 Deamidated (NQ); Oxidation (M)
482 414.1881 826.3617 826.3643 -3.15 1 7 0.91 +1Score > 19 indicates identity RDDMFK + Oxidation (M)
533 427.6943 853.3741 853.3608 15.5 0 7 0.42 +1Score > 16 indicates identity MMSQAMR
838 441.2004 1320.5795 1320.6058 -19.9 1 7 0.28 +1Score > 20 indicates identity
Score > 14 indicates homology
DNNRVGFAEAAR + 2 Deamidated (NQ)
2929 616.7802 1231.5459 1231.5431 2.28 0 7 0.71 +1Score > 18 indicates identity TNFNFAIMEK + 2 Deamidated (NQ); Oxidation (M)
3421 657.2986 1312.5827 1312.5686 10.8 1 7 0.63 +1Score > 21 indicates identity
Score > 18 indicates homology
IMDRMTMNGGR + 2 Oxidation (M)
3816 684.3109 2733.2145 2733.2567 -15.4 0 7 1 +1Score > 20 indicates identity
Score > 20 indicates homology
FTGGSVQVFHGMAAYFGLPEALMNR + 2 Deamidated (NQ); 2 Oxidation (M)
303 400.6820 1598.6989 1598.7001 -0.74 0 7 0.45 +1Score > 16 indicates identity FANSLFVNNWDNR + 3 Deamidated (NQ)
2113 549.2496 2192.9692 2192.9896 -9.29 0 7 0.82 +1Score > 20 indicates identity
Score > 19 indicates homology
EAVLCEWTETVNTPSAQTR + 2 Deamidated (NQ)
450 414.1881 1239.5426 1239.5661 -19.0 0 7 0.94 +1Score > 19 indicates identity MIMAMVQDIR + Deamidated (NQ); 2 Oxidation (M)
2740 603.2741 1204.5337 1204.5281 4.60 0 7 0.23 +1Score > 20 indicates identity
Score > 13 indicates homology
MEQNPQSQLK + 3 Deamidated (NQ)
4357 724.8293 2895.2883 2895.2691 6.62 1 7 0.77 +1Score > 18 indicates identity AIDSISNGYTYFDSVHMDCEKISSR + Deamidated (NQ)
7178 954.4337 1906.8529 1906.8513 0.82 1 7 0.28 +1Score > 21 indicates identity
Score > 14 indicates homology
DDVNEVRNGMECGIGVK + Oxidation (M)
979 454.7066 1814.7972 1814.8219 -13.6 0 7 0.64 +1Score > 17 indicates identity VINGFMIQGGGFEPGMK + 2 Deamidated (NQ); 2 Oxidation (M)
2836 603.2741 1806.8005 1806.8314 -17.1 0 7 0.86 +1Score > 20 indicates identity
Score > 19 indicates homology
TVAAEMPAMQCLQLAR + 2 Deamidated (NQ); Oxidation (M)
70 387.1759 772.3372 772.3464 -11.9 0 7 0.51 +1Score > 16 indicates identity NNPDGTR
2778 603.2741 1204.5337 1204.5281 4.60 0 7 0.58 +1Score > 20 indicates identity
Score > 17 indicates homology
MEQNPQSQLK + 3 Deamidated (NQ)
3265 643.7925 1285.5705 1285.5609 7.53 0 7 0.82 +1Score > 18 indicates identity DNGAEYTMTLR + Oxidation (M)
1788 522.2372 1563.6899 1563.6844 3.55 0 7 1 +1Score > 19 indicates identity MICSPQNNTGAPMK + Oxidation (M)
1799 522.2372 1042.4599 1042.4502 9.37 1 7 1 +1Score > 19 indicates identity ESFSNMRR + Deamidated (NQ); Oxidation (M)
4109 711.3232 1420.6319 1420.6331 -0.82 0 7 0.25 +1Score > 21 indicates identity
Score > 13 indicates homology
HNGGQVIHSADER + 2 Deamidated (NQ)
324 400.6820 1598.6989 1598.7113 -7.76 0 7 0.47 +1Score > 16 indicates identity EHEHEIAWYTER
2316 562.7557 2246.9936 2246.9606 14.7 0 7 0.78 +1Score > 18 indicates identity HNIEAQHMTIDTNGQMVMK + 2 Deamidated (NQ); 3 Oxidation (M)
335 400.6820 1598.6989 1598.6990 -0.051 0 7 0.47 +1Score > 16 indicates identity SDVTPALSEMMQMK + 2 Oxidation (M)
1406 495.2250 1482.6531 1482.6806 -18.6 0 7 0.29 +1Score > 20 indicates identity
Score > 14 indicates homology
MVNSGTEATMSAIR + Oxidation (M)
7508 980.1954 3916.7526 3916.7598 -1.83 1 7 0.78 +1Score > 18 indicates identity GSGIHVVSNQPRHQQAADNNMEFANYGPFELLQAR + 6 Deamidated (NQ); Oxidation (M)
1131 468.2127 934.4109 934.4178 -7.41 0 7 1 +1Score > 20 indicates identity
Score > 19 indicates homology
NAALNEMR + Deamidated (NQ); Oxidation (M)
231 400.6820 799.3495 799.3573 -9.77 0 7 0.47 +1Score > 16 indicates identity HAGNSGTR + Deamidated (NQ)
376 414.1881 1239.5426 1239.5553 -10.3 1 7 1 +1Score > 19 indicates identity QKMAENNGGYK + Deamidated (NQ)
6947 940.9276 1879.8405 1879.8384 1.16 1 7 1 +1Score > 19 indicates identity YVMLHGVNRHDNDHR + 2 Deamidated (NQ); Oxidation (M)
1141 468.2127 1401.6163 1401.6082 5.79 1 7 0.68 +1Score > 20 indicates identity
Score > 18 indicates homology
TATADNKSMFNGK + 2 Deamidated (NQ); Oxidation (M)
1146 468.2127 934.4109 934.4290 -19.5 1 7 1 +1Score > 20 indicates identity
Score > 19 indicates homology
DCSLQRR + Deamidated (NQ)
1267 481.7188 961.4231 961.4141 9.40 0 7 0.76 +1Score > 18 indicates identity NGWENNVK + 2 Deamidated (NQ)
2510 576.2618 1150.5091 1150.4891 17.4 0 7 0.62 +1Score > 20 indicates identity
Score > 17 indicates homology
DEAQEQQFR + Deamidated (NQ)
127 387.1759 1544.6744 1544.7028 -18.4 0 7 0.53 +1Score > 16 indicates identity HAEQENMTLTELK + 2 Deamidated (NQ)
394 414.1881 1239.5426 1239.5554 -10.3 0 7 1 +1Score > 19 indicates identity DGGTQFEVMTR
150 387.1759 1544.6744 1544.6889 -9.41 0 7 0.54 +1Score > 16 indicates identity QAGCTLHEVGTTNR + 2 Deamidated (NQ)
734 441.2004 880.3863 880.3709 17.5 0 7 1 +1Score > 20 indicates identity
Score > 19 indicates homology
ATDSATCR
1325 481.7188 1922.8463 1922.8778 -16.4 0 7 0.77 +1Score > 18 indicates identity SANMTLEQLESLNAEQK + 2 Deamidated (NQ); Oxidation (M)
2280 562.7557 1123.4968 1123.4968 0.0036 0 7 0.82 +1Score > 18 indicates identity HADTAMYTAK + Oxidation (M)
7179 954.4337 1906.8529 1906.8883 -18.6 0 7 0.44 +1Score > 21 indicates identity
Score > 16 indicates homology
WSSQMINTLQIQFHR + 3 Deamidated (NQ); Oxidation (M)
5390 805.8662 3219.4357 3219.4973 -19.1 1 7 0.88 +1Score > 19 indicates identity VAMMNRLQQALEHNHFFLMAQPITGMR + 4 Deamidated (NQ); 2 Oxidation (M)
3169 630.2864 2517.1164 2517.1264 -3.96 0 7 0.37 +1Score > 20 indicates identity
Score > 15 indicates homology
ANGIEHSNAHDAMADVYATIAMAK + Deamidated (NQ); Oxidation (M)
5369 805.8662 1609.7179 1609.7480 -18.7 0 7 0.89 +1Score > 19 indicates identity VFADTGENGVMMPVK + Oxidation (M)
1167 468.2127 934.4109 934.4291 -19.5 1 7 1 +1Score > 20 indicates identity
Score > 19 indicates homology
DGMRVGER + Oxidation (M)
624 427.6943 853.3741 853.3851 -13.0 0 7 0.48 +1Score > 16 indicates identity GEMSTVSK + Oxidation (M)
230 400.6820 1598.6989 1598.6882 6.69 1 7 0.5 +1Score > 16 indicates identity GDLSFMQRESDGEK + Deamidated (NQ)
1352 481.7188 1922.8463 1922.8754 -15.1 1 6 0.79 +1Score > 18 indicates identity FMSRGYSMTQIAEQLK + 2 Deamidated (NQ); 2 Oxidation (M)
1704 522.2372 2084.9199 2084.9585 -18.5 0 6 1.1 +1Score > 19 indicates identity LAVHSAMESLSYHPNANAR + 2 Deamidated (NQ); Oxidation (M)
1570 508.7311 1015.4477 1015.4611 -13.1 0 6 0.62 +1Score > 17 indicates identity YYDAETVR
5805 846.3846 1690.7546 1690.7403 8.47 0 6 1 +1Score > 20 indicates identity
Score > 19 indicates homology
SEMGDLAQSVSHMQR + Oxidation (M)
507 414.1881 1239.5426 1239.5666 -19.4 1 6 1.1 +1Score > 19 indicates identity QQFATMGERR + Deamidated (NQ); Oxidation (M)
1750 522.2372 1563.6899 1563.7160 -16.7 0 6 1.1 +1Score > 19 indicates identity NLMDAIELEQMPK + Deamidated (NQ); 2 Oxidation (M)
401 414.1881 1239.5426 1239.5661 -19.0 0 6 1.1 +1Score > 19 indicates identity SPMELMTMLR + 2 Oxidation (M)
407 414.1881 826.3617 826.3643 -3.13 0 6 1.1 +1Score > 19 indicates identity MYVNQR + Deamidated (NQ); Oxidation (M)
1275 481.7188 961.4231 961.4063 17.6 0 6 0.8 +1Score > 18 indicates identity METPQPDK + Deamidated (NQ); Oxidation (M)
1868 522.2372 1563.6899 1563.7126 -14.5 0 6 1.1 +1Score > 19 indicates identity NAMLPLENSDSPFK + 2 Deamidated (NQ)
6449 900.4092 3597.6076 3597.6790 -19.8 0 6 0.93 +1Score > 20 indicates identity
Score > 19 indicates homology
IDVMLDSDGQFYLLEANTSPGMTSHSLVPMAAR + 2 Oxidation (M)
366 414.1881 1652.7235 1652.7318 -5.03 1 6 1.1 +1Score > 19 indicates identity WYAERDAEIENEK + Deamidated (NQ)
384 414.1881 1239.5426 1239.5554 -10.3 0 6 1.1 +1Score > 19 indicates identity DGGTQFEVMTR
752 441.2004 880.3863 880.3709 17.5 0 6 0.69 +1Score > 20 indicates identity
Score > 17 indicates homology
TQNMGGTR + Deamidated (NQ); Oxidation (M)
1043 468.2127 1401.6163 1401.6194 -2.24 0 6 1 +1Score > 20 indicates identity
Score > 19 indicates homology
TLPPGCEGEEASR
441 414.1881 1652.7235 1652.6956 16.8 1 6 1.1 +1Score > 19 indicates identity CLMCQPASTQGNRK + 3 Deamidated (NQ)
2431 576.2618 1725.7637 1725.7376 15.1 1 6 1 +1Score > 20 indicates identity
Score > 19 indicates homology
EIFNMSNQRNGDQR + 2 Deamidated (NQ); Oxidation (M)
3824 684.3109 1366.6073 1366.5857 15.8 0 6 1 +1Score > 20 indicates identity
Score > 19 indicates homology
EQQLEVMTGCR + Deamidated (NQ); Oxidation (M)
1492 495.2250 988.4354 988.4219 13.7 0 6 1 +1Score > 20 indicates identity
Score > 19 indicates homology
GNHEVMMR + Oxidation (M)
5821 846.3846 1690.7546 1690.7369 10.5 1 6 1 +1Score > 20 indicates identity
Score > 19 indicates homology
QYYMNQQRTQSAR + 2 Deamidated (NQ); Oxidation (M)
1096 468.2127 934.4109 934.4192 -8.88 0 6 0.32 +1Score > 20 indicates identity
Score > 14 indicates homology
HHSPACAR
3692 670.8048 1339.5951 1339.6190 -17.9 1 6 1.1 +1Score > 19 indicates identity KDSGFQMNQLR + Deamidated (NQ); Oxidation (M)
571 427.6943 853.3741 853.3600 16.5 0 6 0.51 +1Score > 16 indicates identity MTSSAGQR + Deamidated (NQ); Oxidation (M)
2851 603.2741 1806.8005 1806.8314 -17.1 0 6 1 +1Score > 20 indicates identity
Score > 19 indicates homology
TVAAEMPAMQCLQLAR + 2 Deamidated (NQ); Oxidation (M)
4356 724.8293 2895.2883 2895.2691 6.62 1 6 0.9 +1Score > 18 indicates identity AIDSISNGYTYFDSVHMDCEKISSR + Deamidated (NQ)
4934 778.8539 1555.6932 1555.7049 -7.50 0 6 0.92 +1Score > 18 indicates identity EGHIQQQMGGINAR + 2 Deamidated (NQ); Oxidation (M)
1512 495.2250 1482.6531 1482.6409 8.23 0 6 1 +1Score > 20 indicates identity
Score > 19 indicates homology
FSSPGQMSGDSLNR + Deamidated (NQ)
7188 954.4337 3813.7058 3813.6842 5.66 1 6 0.6 +1Score > 21 indicates identity
Score > 17 indicates homology
IMTGAPVPEGCEAVVMQEQTEQMDNGVRFTAEVR + 3 Deamidated (NQ); 2 Oxidation (M)
502 414.1881 1652.7235 1652.7273 -2.32 0 6 1.1 +1Score > 19 indicates identity EMVSEMVANDLEAAK + Deamidated (NQ); Oxidation (M)
2727 603.2741 1204.5337 1204.5394 -4.75 1 6 1 +1Score > 20 indicates identity
Score > 19 indicates homology
GDLKNMNPDGK + Deamidated (NQ); Oxidation (M)
1632 508.7311 1015.4477 1015.4619 -14.0 0 6 0.66 +1Score > 17 indicates identity FDMMGFLR
311 400.6820 1598.6989 1598.7259 -16.9 1 6 0.54 +1Score > 16 indicates identity AWAQAAQCPPDRAR + 2 Deamidated (NQ)
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