MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
Repeat search

[help]

Export

Not what you expected? Try the select summary.

Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

Filters

[help]

Show

Unassigned peptides, 2801–2900 (out of 7566)

Page: Previous 1 …24 25 26 27 28 29 30 31 32 33 34 … 76 Next 

Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1488 495.2250 988.4354
1489 495.2250 988.4354
1494 495.2250 988.4354
1495 495.2250 988.4354
1496 495.2250 988.4354
1500 495.2250 988.4354
1501 495.2250 988.4354
1503 495.2250 988.4354
1504 495.2250 988.4354
1506 495.2250 988.4354
1508 495.2250 988.4354
1509 495.2250 988.4354
1510 495.2250 988.4354
1511 495.2250 988.4354
1513 495.2250 988.4354
1514 495.2250 988.4354
1515 495.2250 988.4354
1517 495.2250 988.4354
1518 495.2250 988.4354
1526 495.2250 988.4354
1527 495.2250 988.4354
1528 495.2250 988.4354
1529 495.2250 988.4354
1530 495.2250 988.4354
1531 508.7311 1015.4477
1532 508.7311 1015.4477
1533 508.7311 1015.4477
1534 508.7311 1015.4477
1535 508.7311 1015.4477
1536 508.7311 1015.4477
1537 508.7311 1015.4477
1538 508.7311 1015.4477
1539 508.7311 1015.4477
1540 508.7311 1015.4477
1541 508.7311 1015.4477
1542 508.7311 1015.4477
1543 508.7311 1015.4477
1544 508.7311 1015.4477
1545 508.7311 1015.4477
1549 508.7311 1015.4477
1552 508.7311 1015.4477
1556 508.7311 1015.4477
1557 508.7311 1015.4477
1558 508.7311 1015.4477
1559 508.7311 1015.4477
1560 508.7311 1015.4477
1561 508.7311 1015.4477
1562 508.7311 1015.4477
1564 508.7311 1015.4477
1565 508.7311 1015.4477
1568 508.7311 1015.4477
1572 508.7311 1015.4477
1573 508.7311 1015.4477
1574 508.7311 1015.4477
1575 508.7311 1015.4477
1578 508.7311 1015.4477
1580 508.7311 1015.4477
1581 508.7311 1015.4477
1582 508.7311 1015.4477
1585 508.7311 1015.4477
1586 508.7311 1015.4477
1587 508.7311 1015.4477
1588 508.7311 1015.4477
1590 508.7311 1015.4477
1593 508.7311 1015.4477
1594 508.7311 1015.4477
1595 508.7311 1015.4477
1596 508.7311 1015.4477
1597 508.7311 1015.4477
1598 508.7311 1015.4477
1600 508.7311 1015.4477
1601 508.7311 1015.4477
1602 508.7311 1015.4477
1604 508.7311 1015.4477
1608 508.7311 1015.4477
1611 508.7311 1015.4477
1612 508.7311 1015.4477
1614 508.7311 1015.4477
1615 508.7311 1015.4477
1616 508.7311 1015.4477
1619 508.7311 1015.4477
1621 508.7311 1015.4477
1622 508.7311 1015.4477
1623 508.7311 1015.4477
1628 508.7311 1015.4477
1629 508.7311 1015.4477
1630 508.7311 1015.4477
1631 508.7311 1015.4477
1633 508.7311 1015.4477
1634 508.7311 1015.4477
1636 508.7311 1015.4477
1637 508.7311 1015.4477
1638 508.7311 1015.4477
1639 508.7311 1015.4477
1643 508.7311 1015.4477
1644 508.7311 1015.4477
1646 508.7311 1015.4477
1647 508.7311 1015.4477
1648 508.7311 1015.4477
1649 508.7311 1015.4477
Page: Previous 1 …24 25 26 27 28 29 30 31 32 33 34 … 76 Next 

Not what you expected? Try the select summary.

Mascot: http://www.matrixscience.com/