MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2701–2800 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1333 481.7188 961.4231
1334 481.7188 961.4231
1335 481.7188 961.4231
1336 481.7188 961.4231
1337 481.7188 961.4231
1338 481.7188 961.4231
1339 481.7188 961.4231
1340 481.7188 961.4231
1341 481.7188 961.4231
1342 481.7188 961.4231
1343 481.7188 961.4231
1344 481.7188 961.4231
1346 481.7188 961.4231
1347 481.7188 961.4231
1348 481.7188 961.4231
1350 481.7188 961.4231
1351 481.7188 961.4231
1353 481.7188 961.4231
1354 481.7188 961.4231
1355 481.7188 961.4231
1357 481.7188 961.4231
1358 481.7188 961.4231
1360 481.7188 961.4231
1361 495.2250 988.4354
1362 495.2250 988.4354
1363 495.2250 988.4354
1364 495.2250 988.4354
1365 495.2250 988.4354
1366 495.2250 988.4354
1367 495.2250 988.4354
1368 495.2250 988.4354
1369 495.2250 988.4354
1370 495.2250 988.4354
1371 495.2250 988.4354
1372 495.2250 988.4354
1373 495.2250 988.4354
1374 495.2250 988.4354
1375 495.2250 988.4354
1376 495.2250 988.4354
1378 495.2250 988.4354
1379 495.2250 988.4354
1380 495.2250 988.4354
1382 495.2250 988.4354
1388 495.2250 988.4354
1390 495.2250 988.4354
1391 495.2250 988.4354
1393 495.2250 988.4354
1394 495.2250 988.4354
1396 495.2250 988.4354
1397 495.2250 988.4354
1399 495.2250 988.4354
1400 495.2250 988.4354
1403 495.2250 988.4354
1405 495.2250 988.4354
1407 495.2250 988.4354
1408 495.2250 988.4354
1409 495.2250 988.4354
1410 495.2250 988.4354
1412 495.2250 988.4354
1414 495.2250 988.4354
1418 495.2250 988.4354
1419 495.2250 988.4354
1421 495.2250 988.4354
1422 495.2250 988.4354
1425 495.2250 988.4354
1431 495.2250 988.4354
1432 495.2250 988.4354
1433 495.2250 988.4354
1435 495.2250 988.4354
1437 495.2250 988.4354
1439 495.2250 988.4354
1440 495.2250 988.4354
1442 495.2250 988.4354
1443 495.2250 988.4354
1444 495.2250 988.4354
1445 495.2250 988.4354
1446 495.2250 988.4354
1447 495.2250 988.4354
1448 495.2250 988.4354
1449 495.2250 988.4354
1454 495.2250 988.4354
1455 495.2250 988.4354
1456 495.2250 988.4354
1457 495.2250 988.4354
1458 495.2250 988.4354
1463 495.2250 988.4354
1464 495.2250 988.4354
1466 495.2250 988.4354
1467 495.2250 988.4354
1470 495.2250 988.4354
1471 495.2250 988.4354
1475 495.2250 988.4354
1476 495.2250 988.4354
1478 495.2250 988.4354
1479 495.2250 988.4354
1480 495.2250 988.4354
1482 495.2250 988.4354
1484 495.2250 988.4354
1485 495.2250 988.4354
1487 495.2250 988.4354
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