MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2601–2700 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1197 481.7188 961.4231
1198 481.7188 961.4231
1199 481.7188 961.4231
1200 481.7188 961.4231
1201 481.7188 961.4231
1202 481.7188 961.4231
1203 481.7188 961.4231
1207 481.7188 961.4231
1208 481.7188 961.4231
1209 481.7188 961.4231
1210 481.7188 961.4231
1211 481.7188 961.4231
1212 481.7188 961.4231
1213 481.7188 961.4231
1216 481.7188 961.4231
1217 481.7188 961.4231
1218 481.7188 961.4231
1219 481.7188 961.4231
1220 481.7188 961.4231
1222 481.7188 961.4231
1223 481.7188 961.4231
1224 481.7188 961.4231
1225 481.7188 961.4231
1226 481.7188 961.4231
1227 481.7188 961.4231
1228 481.7188 961.4231
1229 481.7188 961.4231
1230 481.7188 961.4231
1231 481.7188 961.4231
1233 481.7188 961.4231
1234 481.7188 961.4231
1235 481.7188 961.4231
1236 481.7188 961.4231
1237 481.7188 961.4231
1238 481.7188 961.4231
1240 481.7188 961.4231
1244 481.7188 961.4231
1245 481.7188 961.4231
1246 481.7188 961.4231
1247 481.7188 961.4231
1249 481.7188 961.4231
1251 481.7188 961.4231
1252 481.7188 961.4231
1255 481.7188 961.4231
1256 481.7188 961.4231
1257 481.7188 961.4231
1258 481.7188 961.4231
1259 481.7188 961.4231
1260 481.7188 961.4231
1261 481.7188 961.4231
1262 481.7188 961.4231
1263 481.7188 961.4231
1264 481.7188 961.4231
1265 481.7188 961.4231
1269 481.7188 961.4231
1270 481.7188 961.4231
1271 481.7188 961.4231
1272 481.7188 961.4231
1274 481.7188 961.4231
1276 481.7188 961.4231
1277 481.7188 961.4231
1278 481.7188 961.4231
1279 481.7188 961.4231
1281 481.7188 961.4231
1283 481.7188 961.4231
1284 481.7188 961.4231
1285 481.7188 961.4231
1286 481.7188 961.4231
1289 481.7188 961.4231
1290 481.7188 961.4231
1291 481.7188 961.4231
1292 481.7188 961.4231
1293 481.7188 961.4231
1294 481.7188 961.4231
1295 481.7188 961.4231
1296 481.7188 961.4231
1298 481.7188 961.4231
1299 481.7188 961.4231
1300 481.7188 961.4231
1301 481.7188 961.4231
1303 481.7188 961.4231
1304 481.7188 961.4231
1305 481.7188 961.4231
1306 481.7188 961.4231
1309 481.7188 961.4231
1311 481.7188 961.4231
1312 481.7188 961.4231
1313 481.7188 961.4231
1314 481.7188 961.4231
1315 481.7188 961.4231
1318 481.7188 961.4231
1319 481.7188 961.4231
1320 481.7188 961.4231
1321 481.7188 961.4231
1323 481.7188 961.4231
1326 481.7188 961.4231
1327 481.7188 961.4231
1329 481.7188 961.4231
1331 481.7188 961.4231
1332 481.7188 961.4231
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