MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2501–2600 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1041 468.2127 934.4109
1042 468.2127 934.4109
1044 468.2127 934.4109
1045 468.2127 934.4109
1046 468.2127 934.4109
1047 468.2127 934.4109
1048 468.2127 934.4109
1050 468.2127 934.4109
1052 468.2127 934.4109
1053 468.2127 934.4109
1054 468.2127 934.4109
1055 468.2127 934.4109
1057 468.2127 934.4109
1060 468.2127 934.4109
1061 468.2127 934.4109
1062 468.2127 934.4109
1063 468.2127 934.4109
1064 468.2127 934.4109
1065 468.2127 934.4109
1066 468.2127 934.4109
1067 468.2127 934.4109
1068 468.2127 934.4109
1069 468.2127 934.4109
1070 468.2127 934.4109
1071 468.2127 934.4109
1073 468.2127 934.4109
1074 468.2127 934.4109
1076 468.2127 934.4109
1077 468.2127 934.4109
1078 468.2127 934.4109
1080 468.2127 934.4109
1082 468.2127 934.4109
1084 468.2127 934.4109
1085 468.2127 934.4109
1087 468.2127 934.4109
1089 468.2127 934.4109
1090 468.2127 934.4109
1092 468.2127 934.4109
1098 468.2127 934.4109
1099 468.2127 934.4109
1100 468.2127 934.4109
1101 468.2127 934.4109
1102 468.2127 934.4109
1103 468.2127 934.4109
1104 468.2127 934.4109
1106 468.2127 934.4109
1108 468.2127 934.4109
1109 468.2127 934.4109
1111 468.2127 934.4109
1112 468.2127 934.4109
1113 468.2127 934.4109
1115 468.2127 934.4109
1116 468.2127 934.4109
1120 468.2127 934.4109
1121 468.2127 934.4109
1122 468.2127 934.4109
1124 468.2127 934.4109
1125 468.2127 934.4109
1126 468.2127 934.4109
1128 468.2127 934.4109
1132 468.2127 934.4109
1136 468.2127 934.4109
1137 468.2127 934.4109
1139 468.2127 934.4109
1140 468.2127 934.4109
1144 468.2127 934.4109
1145 468.2127 934.4109
1147 468.2127 934.4109
1148 468.2127 934.4109
1149 468.2127 934.4109
1151 468.2127 934.4109
1152 468.2127 934.4109
1153 468.2127 934.4109
1154 468.2127 934.4109
1156 468.2127 934.4109
1160 468.2127 934.4109
1166 468.2127 934.4109
1169 468.2127 934.4109
1170 468.2127 934.4109
1171 468.2127 934.4109
1173 468.2127 934.4109
1174 468.2127 934.4109
1176 468.2127 934.4109
1177 468.2127 934.4109
1178 468.2127 934.4109
1180 468.2127 934.4109
1181 468.2127 934.4109
1182 468.2127 934.4109
1183 468.2127 934.4109
1184 468.2127 934.4109
1186 468.2127 934.4109
1187 468.2127 934.4109
1188 468.2127 934.4109
1189 468.2127 934.4109
1190 468.2127 934.4109
1191 481.7188 961.4231
1192 481.7188 961.4231
1193 481.7188 961.4231
1194 481.7188 961.4231
1195 481.7188 961.4231
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