MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2401–2500 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
892 454.7066 907.3986
893 454.7066 907.3986
894 454.7066 907.3986
895 454.7066 907.3986
899 454.7066 907.3986
902 454.7066 907.3986
903 454.7066 907.3986
904 454.7066 907.3986
905 454.7066 907.3986
906 454.7066 907.3986
907 454.7066 907.3986
909 454.7066 907.3986
910 454.7066 907.3986
911 454.7066 907.3986
912 454.7066 907.3986
914 454.7066 907.3986
918 454.7066 907.3986
919 454.7066 907.3986
920 454.7066 907.3986
921 454.7066 907.3986
923 454.7066 907.3986
927 454.7066 907.3986
933 454.7066 907.3986
934 454.7066 907.3986
935 454.7066 907.3986
939 454.7066 907.3986
941 454.7066 907.3986
943 454.7066 907.3986
944 454.7066 907.3986
945 454.7066 907.3986
947 454.7066 907.3986
948 454.7066 907.3986
950 454.7066 907.3986
951 454.7066 907.3986
952 454.7066 907.3986
953 454.7066 907.3986
954 454.7066 907.3986
955 454.7066 907.3986
957 454.7066 907.3986
958 454.7066 907.3986
959 454.7066 907.3986
960 454.7066 907.3986
962 454.7066 907.3986
964 454.7066 907.3986
965 454.7066 907.3986
969 454.7066 907.3986
970 454.7066 907.3986
971 454.7066 907.3986
973 454.7066 907.3986
981 454.7066 907.3986
982 454.7066 907.3986
983 454.7066 907.3986
984 454.7066 907.3986
986 454.7066 907.3986
988 454.7066 907.3986
989 454.7066 907.3986
991 454.7066 907.3986
992 454.7066 907.3986
993 454.7066 907.3986
994 454.7066 907.3986
995 454.7066 907.3986
996 454.7066 907.3986
997 454.7066 907.3986
998 454.7066 907.3986
999 454.7066 907.3986
1000 454.7066 907.3986
1001 454.7066 907.3986
1002 454.7066 907.3986
1003 454.7066 907.3986
1008 454.7066 907.3986
1009 454.7066 907.3986
1011 454.7066 907.3986
1012 454.7066 907.3986
1013 454.7066 907.3986
1014 454.7066 907.3986
1015 454.7066 907.3986
1016 454.7066 907.3986
1017 454.7066 907.3986
1018 454.7066 907.3986
1020 454.7066 907.3986
1021 468.2127 934.4109
1022 468.2127 934.4109
1023 468.2127 934.4109
1024 468.2127 934.4109
1025 468.2127 934.4109
1026 468.2127 934.4109
1027 468.2127 934.4109
1028 468.2127 934.4109
1029 468.2127 934.4109
1030 468.2127 934.4109
1031 468.2127 934.4109
1032 468.2127 934.4109
1033 468.2127 934.4109
1034 468.2127 934.4109
1035 468.2127 934.4109
1036 468.2127 934.4109
1037 468.2127 934.4109
1038 468.2127 934.4109
1039 468.2127 934.4109
1040 468.2127 934.4109
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