MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2201–2300 (out of 7566)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
572 427.6943 853.3741
573 427.6943 853.3741
574 427.6943 853.3741
575 427.6943 853.3741
576 427.6943 853.3741
581 427.6943 853.3741
583 427.6943 853.3741
584 427.6943 853.3741
585 427.6943 853.3741
587 427.6943 853.3741
588 427.6943 853.3741
589 427.6943 853.3741
590 427.6943 853.3741
591 427.6943 853.3741
592 427.6943 853.3741
593 427.6943 853.3741
595 427.6943 853.3741
597 427.6943 853.3741
598 427.6943 853.3741
603 427.6943 853.3741
604 427.6943 853.3741
605 427.6943 853.3741
606 427.6943 853.3741
608 427.6943 853.3741
609 427.6943 853.3741
610 427.6943 853.3741
614 427.6943 853.3741
615 427.6943 853.3741
616 427.6943 853.3741
617 427.6943 853.3741
618 427.6943 853.3741
620 427.6943 853.3741
621 427.6943 853.3741
623 427.6943 853.3741
625 427.6943 853.3741
626 427.6943 853.3741
627 427.6943 853.3741
630 427.6943 853.3741
632 427.6943 853.3741
636 427.6943 853.3741
644 427.6943 853.3741
646 427.6943 853.3741
647 427.6943 853.3741
648 427.6943 853.3741
650 427.6943 853.3741
651 427.6943 853.3741
652 427.6943 853.3741
653 427.6943 853.3741
654 427.6943 853.3741
655 427.6943 853.3741
656 427.6943 853.3741
657 427.6943 853.3741
659 427.6943 853.3741
661 427.6943 853.3741
662 427.6943 853.3741
663 427.6943 853.3741
665 427.6943 853.3741
666 427.6943 853.3741
667 427.6943 853.3741
668 427.6943 853.3741
669 427.6943 853.3741
670 427.6943 853.3741
671 427.6943 853.3741
672 427.6943 853.3741
674 427.6943 853.3741
677 427.6943 853.3741
678 427.6943 853.3741
679 427.6943 853.3741
680 427.6943 853.3741
681 441.2004 880.3863
682 441.2004 880.3863
683 441.2004 880.3863
684 441.2004 880.3863
685 441.2004 880.3863
686 441.2004 880.3863
687 441.2004 880.3863
688 441.2004 880.3863
689 441.2004 880.3863
690 441.2004 880.3863
691 441.2004 880.3863
692 441.2004 880.3863
693 441.2004 880.3863
694 441.2004 880.3863
695 441.2004 880.3863
696 441.2004 880.3863
697 441.2004 880.3863
700 441.2004 880.3863
702 441.2004 880.3863
703 441.2004 880.3863
704 441.2004 880.3863
705 441.2004 880.3863
707 441.2004 880.3863
709 441.2004 880.3863
711 441.2004 880.3863
712 441.2004 880.3863
713 441.2004 880.3863
715 441.2004 880.3863
716 441.2004 880.3863
717 441.2004 880.3863
719 441.2004 880.3863
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