MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2101–2200 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
337 400.6820 799.3495
338 400.6820 799.3495
339 400.6820 799.3495
341 414.1881 826.3617
342 414.1881 826.3617
343 414.1881 826.3617
344 414.1881 826.3617
345 414.1881 826.3617
346 414.1881 826.3617
347 414.1881 826.3617
348 414.1881 826.3617
349 414.1881 826.3617
350 414.1881 826.3617
351 414.1881 826.3617
352 414.1881 826.3617
353 414.1881 826.3617
354 414.1881 826.3617
355 414.1881 826.3617
356 414.1881 826.3617
377 414.1881 826.3617
378 414.1881 826.3617
380 414.1881 826.3617
387 414.1881 826.3617
388 414.1881 826.3617
398 414.1881 826.3617
405 414.1881 826.3617
406 414.1881 826.3617
411 414.1881 826.3617
414 414.1881 826.3617
415 414.1881 826.3617
417 414.1881 826.3617
418 414.1881 826.3617
419 414.1881 826.3617
421 414.1881 826.3617
434 414.1881 826.3617
435 414.1881 826.3617
436 414.1881 826.3617
444 414.1881 826.3617
454 414.1881 826.3617
455 414.1881 826.3617
457 414.1881 826.3617
458 414.1881 826.3617
460 414.1881 826.3617
461 414.1881 826.3617
463 414.1881 826.3617
467 414.1881 826.3617
471 414.1881 826.3617
473 414.1881 826.3617
476 414.1881 826.3617
479 414.1881 826.3617
481 414.1881 826.3617
483 414.1881 826.3617
484 414.1881 826.3617
487 414.1881 826.3617
490 414.1881 826.3617
492 414.1881 826.3617
493 414.1881 826.3617
498 414.1881 826.3617
500 414.1881 826.3617
501 414.1881 826.3617
508 414.1881 826.3617
511 427.6943 853.3741
512 427.6943 853.3741
513 427.6943 853.3741
514 427.6943 853.3741
515 427.6943 853.3741
516 427.6943 853.3741
517 427.6943 853.3741
518 427.6943 853.3741
519 427.6943 853.3741
520 427.6943 853.3741
521 427.6943 853.3741
522 427.6943 853.3741
523 427.6943 853.3741
524 427.6943 853.3741
525 427.6943 853.3741
526 427.6943 853.3741
528 427.6943 853.3741
529 427.6943 853.3741
531 427.6943 853.3741
534 427.6943 853.3741
535 427.6943 853.3741
537 427.6943 853.3741
540 427.6943 853.3741
541 427.6943 853.3741
542 427.6943 853.3741
543 427.6943 853.3741
544 427.6943 853.3741
545 427.6943 853.3741
546 427.6943 853.3741
549 427.6943 853.3741
550 427.6943 853.3741
552 427.6943 853.3741
559 427.6943 853.3741
560 427.6943 853.3741
561 427.6943 853.3741
562 427.6943 853.3741
567 427.6943 853.3741
568 427.6943 853.3741
570 427.6943 853.3741
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