MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2001–2100 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
144 387.1759 772.3372
146 387.1759 772.3372
148 387.1759 772.3372
159 387.1759 772.3372
160 387.1759 772.3372
161 387.1759 772.3372
162 387.1759 772.3372
163 387.1759 772.3372
166 387.1759 772.3372
170 387.1759 772.3372
171 400.6820 799.3495
172 400.6820 799.3495
173 400.6820 799.3495
174 400.6820 799.3495
175 400.6820 799.3495
176 400.6820 799.3495
177 400.6820 799.3495
178 400.6820 799.3495
179 400.6820 799.3495
180 400.6820 799.3495
181 400.6820 799.3495
182 400.6820 799.3495
183 400.6820 799.3495
184 400.6820 799.3495
185 400.6820 799.3495
186 400.6820 799.3495
187 400.6820 799.3495
188 400.6820 799.3495
189 400.6820 799.3495
190 400.6820 799.3495
191 400.6820 799.3495
192 400.6820 799.3495
193 400.6820 799.3495
195 400.6820 799.3495
196 400.6820 799.3495
197 400.6820 799.3495
199 400.6820 799.3495
200 400.6820 799.3495
201 400.6820 799.3495
202 400.6820 799.3495
203 400.6820 799.3495
204 400.6820 799.3495
205 400.6820 799.3495
208 400.6820 799.3495
209 400.6820 799.3495
210 400.6820 799.3495
213 400.6820 799.3495
215 400.6820 799.3495
217 400.6820 799.3495
220 400.6820 799.3495
221 400.6820 799.3495
222 400.6820 799.3495
224 400.6820 799.3495
226 400.6820 799.3495
233 400.6820 799.3495
234 400.6820 799.3495
236 400.6820 799.3495
239 400.6820 799.3495
241 400.6820 799.3495
242 400.6820 799.3495
249 400.6820 799.3495
252 400.6820 799.3495
253 400.6820 799.3495
260 400.6820 799.3495
261 400.6820 799.3495
265 400.6820 799.3495
267 400.6820 799.3495
271 400.6820 799.3495
276 400.6820 799.3495
278 400.6820 799.3495
279 400.6820 799.3495
282 400.6820 799.3495
283 400.6820 799.3495
284 400.6820 799.3495
286 400.6820 799.3495
287 400.6820 799.3495
288 400.6820 799.3495
289 400.6820 799.3495
292 400.6820 799.3495
293 400.6820 799.3495
294 400.6820 799.3495
295 400.6820 799.3495
297 400.6820 799.3495
300 400.6820 799.3495
305 400.6820 799.3495
306 400.6820 799.3495
307 400.6820 799.3495
313 400.6820 799.3495
314 400.6820 799.3495
316 400.6820 799.3495
317 400.6820 799.3495
318 400.6820 799.3495
319 400.6820 799.3495
322 400.6820 799.3495
325 400.6820 799.3495
327 400.6820 799.3495
328 400.6820 799.3495
330 400.6820 799.3495
332 400.6820 799.3495
334 400.6820 799.3495
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