MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 101–200 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2081 549.2496 1096.4846 1096.5036 -17.4 1 10 0.23 +1Score > 20 indicates identity
Score > 16 indicates homology		 KYGPDSSTNK + Deamidated (NQ)
255 400.6820 1598.6989 1598.7186 -12.3 1 10 0.25 +1Score > 16 indicates identity GGQHSGGNFKNDPQR + Deamidated (NQ)
1196 481.7188 961.4231 961.4063 17.6 0 9 0.4 +1Score > 18 indicates identity METPQPDK + Deamidated (NQ); Oxidation (M)
396 414.1881 1239.5426 1239.5554 -10.3 0 9 0.53 +1Score > 19 indicates identity DQYAGAVETMR
1801 522.2372 1563.6899 1563.6909 -0.61 1 9 0.55 +1Score > 19 indicates identity ICSPQNNTGAPMKK + 3 Deamidated (NQ); Oxidation (M)
4216 711.3232 1420.6319 1420.6187 9.31 1 9 0.14 +1Score > 21 indicates identity
Score > 13 indicates homology		 MRAQNCVGPNTR + 2 Deamidated (NQ); Oxidation (M)
1550 508.7311 2030.8955 2030.9190 -11.6 1 9 0.31 +1Score > 17 indicates identity GRVYAVSMFNPQGNDMTK + Deamidated (NQ); Oxidation (M)
4748 751.8416 1501.6687 1501.6620 4.45 1 9 0.49 +1Score > 19 indicates identity FDDRGDLSFMQR + Oxidation (M)
5457 819.3723 1636.7301 1636.7185 7.07 0 9 0.25 +1Score > 21 indicates identity
Score > 16 indicates homology		 VGGLSVMCEDAEAAGR + Oxidation (M)
1830 522.2372 2084.9199 2084.9287 -4.22 0 9 0.55 +1Score > 19 indicates identity QTSLNGFYQDNGGDADLLR + 2 Deamidated (NQ)
1164 468.2127 934.4109 934.4192 -8.88 0 9 0.31 +1Score > 20 indicates identity
Score > 17 indicates homology		 HHSPACAR
4665 751.8416 1501.6687 1501.6620 4.45 1 9 0.51 +1Score > 19 indicates identity FDDRGDLSFMQR + Oxidation (M)
4103 711.3232 1420.6319 1420.6504 -13.0 0 9 0.17 +1Score > 21 indicates identity
Score > 14 indicates homology		 ENITNLDACITR + 2 Deamidated (NQ)
2191 549.2496 2192.9692 2193.0017 -14.8 1 9 0.63 +1Score > 20 indicates identity IAFVCDAGMGSSAMGATTFRK + Oxidation (M)
5286 805.8662 1609.7179 1609.7107 4.44 0 9 0.48 +1Score > 19 indicates identity TLNSNQFDNEDAIK + 2 Deamidated (NQ)
1359 481.7188 961.4231 961.4366 -14.0 0 9 0.42 +1Score > 18 indicates identity QHTHDAPR + Deamidated (NQ)
386 414.1881 1239.5426 1239.5554 -10.3 0 9 0.57 +1Score > 19 indicates identity DGGTQFEVMTR
745 441.2004 1320.5795 1320.6020 -17.0 0 9 0.32 +1Score > 20 indicates identity
Score > 17 indicates homology		 AFNEMQPIVDR + 2 Deamidated (NQ)
1627 508.7311 1015.4477 1015.4570 -9.15 0 9 0.34 +1Score > 17 indicates identity TINNAPDNR + 2 Deamidated (NQ)
238 400.6820 799.3495 799.3460 4.28 0 9 0.27 +1Score > 16 indicates identity ATDDHNK
1401 495.2250 988.4354 988.4219 13.7 0 9 0.65 +1Score > 20 indicates identity GNHEVMMR + Oxidation (M)
3022 616.7802 2463.0917 2463.0570 14.1 1 9 0.45 +1Score > 18 indicates identity LNKEMVEGMGGTQSEQYQEFR + 3 Deamidated (NQ)
1385 495.2250 988.4354 988.4219 13.7 0 9 0.22 +1Score > 20 indicates identity
Score > 15 indicates homology		 GNHEVMMR + Oxidation (M)
3987 697.8171 1393.6196 1393.6329 -9.58 1 9 0.6 +1Score > 19 indicates identity QAQQMQEKMQK + Deamidated (NQ); Oxidation (M)
1345 481.7188 961.4231 961.4063 17.6 0 9 0.44 +1Score > 18 indicates identity METPQPDK + Deamidated (NQ); Oxidation (M)
6851 927.4214 2779.2425 2779.2325 3.60 1 9 1 +1Score > 21 indicates identity
Score > 21 indicates homology		 MMSECIYQEGNAFVIMGAGEQLKR + 2 Deamidated (NQ); Oxidation (M)
430 414.1881 1239.5426 1239.5661 -19.0 0 9 0.6 +1Score > 19 indicates identity SPMELMTMLR + 2 Oxidation (M)
1960 535.7434 1069.4723 1069.4862 -13.1 0 9 0.42 +1Score > 18 indicates identity YCVNSASLR + Deamidated (NQ)
164 387.1759 1544.6744 1544.6994 -16.2 0 9 0.31 +1Score > 16 indicates identity EQNEPVAQVSQWK + 3 Deamidated (NQ)
5810 846.3846 1690.7546 1690.7403 8.47 0 9 0.17 +1Score > 20 indicates identity
Score > 14 indicates homology		 SEMGDLAQSVSHMQR + Oxidation (M)
1253 481.7188 961.4231 961.4141 9.40 0 9 0.45 +1Score > 18 indicates identity NGWENNVK + 2 Deamidated (NQ)
3078 630.2864 2517.1164 2517.1127 1.48 0 9 0.59 +1Score > 20 indicates identity
Score > 19 indicates homology		 MFSMHNCQINPGAGPEITTPWK + 2 Deamidated (NQ)
228 400.6820 1598.6989 1598.7107 -7.36 1 9 0.3 +1Score > 16 indicates identity TGQICQSQRHRPQ + 4 Deamidated (NQ)
5132 792.3600 2374.0583 2374.0899 -13.3 0 9 0.2 +1Score > 21 indicates identity
Score > 14 indicates homology		 LSANWMAAAGHPGEDAGLYEAVK + Deamidated (NQ); Oxidation (M)
281 400.6820 1598.6989 1598.6990 -0.051 0 9 0.3 +1Score > 16 indicates identity SDVTPALSEMMQMK + 2 Oxidation (M)
1328 481.7188 1922.8463 1922.8767 -15.8 1 9 0.47 +1Score > 18 indicates identity YAMNFSADAFMRQISR + Oxidation (M)
2253 562.7557 1123.4968 1123.4968 0.031 1 9 0.5 +1Score > 18 indicates identity MREYPNGEK + Deamidated (NQ)
6058 859.8907 1717.7669 1717.8006 -19.6 1 9 0.51 +1Score > 18 indicates identity SQILDEAKAEAEQER + 2 Deamidated (NQ)
404 414.1881 1239.5426 1239.5661 -19.0 0 9 0.63 +1Score > 19 indicates identity SPMELMTMLR + 2 Oxidation (M)
424 414.1881 1239.5426 1239.5661 -19.0 0 9 0.63 +1Score > 19 indicates identity SPMELMTMLR + 2 Oxidation (M)
2610 589.7679 2355.0427 2355.0796 -15.7 1 9 0.59 +1Score > 19 indicates identity NFVLMQQKMAAGENPAAEMIK + 3 Deamidated (NQ); 2 Oxidation (M)
5461 819.3723 2455.0951 2455.0705 10.0 1 9 0.28 +1Score > 21 indicates identity
Score > 16 indicates homology		 DALPLMENAYRQMQMNAQAEK + 4 Deamidated (NQ)
925 454.7066 907.3986 907.3891 10.4 0 9 0.43 +1Score > 17 indicates identity DMAMNQAK
2120 549.2496 1096.4846 1096.4753 8.44 1 9 0.26 +1Score > 20 indicates identity
Score > 15 indicates homology		 GDARMMASSR + Oxidation (M)
1430 495.2250 1482.6531 1482.6820 -19.5 1 9 0.55 +1Score > 20 indicates identity
Score > 18 indicates homology		 GYDRAMGARPMAR + 2 Oxidation (M)
371 414.1881 1652.7235 1652.7253 -1.09 0 9 0.65 +1Score > 19 indicates identity CWQEEAYAEAQLR
3256 643.7925 2571.1411 2571.1322 3.43 0 9 0.55 +1Score > 18 indicates identity LPFNNGQETNELELMNATFDEK + 2 Deamidated (NQ); Oxidation (M)
5865 846.3846 2536.1319 2536.1363 -1.71 0 9 0.81 +1Score > 20 indicates identity QGMTLEVMVPQGHFGYQPQAER + 2 Deamidated (NQ); 2 Oxidation (M)
635 427.6943 1706.7481 1706.7821 -19.9 0 9 0.3 +1Score > 16 indicates identity HEDMYTAINELINK + Deamidated (NQ); Oxidation (M)
5188 792.3600 2374.0583 2374.0127 19.2 0 8 0.99 +1Score > 21 indicates identity NINVMQESSVGPALSMNFDMR + 3 Deamidated (NQ); 2 Oxidation (M)
6226 873.3969 2617.1689 2617.1238 17.2 0 8 1 +1Score > 22 indicates identity
Score > 21 indicates homology		 NPVSGEVLWQGNDADAAQVEQACR + 4 Deamidated (NQ)
2626 589.7679 1177.5213 1177.5437 -19.0 0 8 0.63 +1Score > 19 indicates identity MFPDSPAAAGSK
4458 738.3355 1474.6565 1474.6524 2.78 1 8 0.22 +1Score > 20 indicates identity
Score > 14 indicates homology		 NMAWRHNPNYR + Deamidated (NQ); Oxidation (M)
427 414.1881 1239.5426 1239.5661 -19.0 0 8 0.68 +1Score > 19 indicates identity SPMELMTMLR + 2 Oxidation (M)
227 400.6820 799.3495 799.3460 4.28 0 8 0.33 +1Score > 16 indicates identity ATDDHNK
1282 481.7188 961.4231 961.4141 9.39 0 8 0.51 +1Score > 18 indicates identity LNGWDNDK + Deamidated (NQ)
1918 535.7434 2138.9445 2138.9466 -0.97 1 8 0.48 +1Score > 18 indicates identity MANLSGYNFAYLDEQTKR + 3 Deamidated (NQ); Oxidation (M)
379 414.1881 1652.7235 1652.7278 -2.62 1 8 0.7 +1Score > 19 indicates identity RFNTANDDNVTQVR + 4 Deamidated (NQ)
6909 927.4214 2779.2425 2779.2476 -1.83 1 8 1 +1Score > 21 indicates identity
Score > 21 indicates homology		 KQTNHAMINNMDFEGNNSCLVVSR + Deamidated (NQ)
2348 562.7557 1123.4968 1123.4824 12.8 1 8 0.56 +1Score > 18 indicates identity RQMMGMEPK + Deamidated (NQ); Oxidation (M)
5875 846.3846 1690.7546 1690.7403 8.47 0 8 0.29 +1Score > 20 indicates identity
Score > 15 indicates homology		 SEMGDLAQSVSHMQR + Oxidation (M)
755 441.2004 1320.5795 1320.6020 -17.0 0 8 0.38 +1Score > 20 indicates identity
Score > 17 indicates homology		 AFNEMQPIVDR + 2 Deamidated (NQ)
321 400.6820 799.3495 799.3460 4.28 0 8 0.34 +1Score > 16 indicates identity ATDDHNK
942 454.7066 907.3986 907.3957 3.23 0 8 0.47 +1Score > 17 indicates identity ELMEGNAK + Deamidated (NQ); Oxidation (M)
1324 481.7188 961.4231 961.4141 9.40 0 8 0.53 +1Score > 18 indicates identity NGWENNVK + 2 Deamidated (NQ)
4550 738.3355 1474.6565 1474.6762 -13.4 0 8 0.81 +1Score > 20 indicates identity MQFYSTPGTGTIR + Deamidated (NQ); Oxidation (M)
3763 684.3109 1366.6073 1366.6299 -16.6 1 8 0.18 +1Score > 20 indicates identity
Score > 13 indicates homology		 SRDAFIDEMQR
64 387.1759 772.3372 772.3351 2.67 0 8 0.38 +1Score > 16 indicates identity AQGEDPR + Deamidated (NQ)
846 441.2004 880.3863 880.3969 -12.0 0 8 0.34 +1Score > 20 indicates identity
Score > 16 indicates homology		 QAMMAAMK
3418 657.2986 1312.5827 1312.5825 0.17 0 8 0.25 +1Score > 21 indicates identity
Score > 15 indicates homology		 VSMPEITAMMR + 3 Oxidation (M)
2755 603.2741 1204.5337 1204.5281 4.60 0 8 0.34 +1Score > 20 indicates identity
Score > 16 indicates homology		 MEQNPQSQLK + 3 Deamidated (NQ)
438 414.1881 1239.5426 1239.5661 -19.0 0 8 0.74 +1Score > 19 indicates identity SPMELMTMLR + 2 Oxidation (M)
3149 630.2864 1887.8373 1887.8105 14.2 1 8 0.81 +1Score > 20 indicates identity EHGFQCHVGSMRSPSR + Deamidated (NQ); Oxidation (M)
774 441.2004 1320.5795 1320.6033 -18.0 1 8 0.86 +1Score > 20 indicates identity WYSAQHRMNK + Deamidated (NQ)
459 414.1881 1239.5426 1239.5661 -19.0 0 8 0.74 +1Score > 19 indicates identity SPMELMTMLR + 2 Oxidation (M)
5767 846.3846 1690.7546 1690.7330 12.8 0 8 0.92 +1Score > 20 indicates identity AMLNIANMGYFSSDR + 2 Deamidated (NQ)
980 454.7066 1814.7972 1814.7902 3.85 0 8 0.5 +1Score > 17 indicates identity CLNHMFAAEIYMQR + 2 Oxidation (M)
422 414.1881 826.3617 826.3643 -3.15 0 8 0.75 +1Score > 19 indicates identity TAMGEFR + Oxidation (M)
676 427.6943 853.3741 853.3851 -13.0 0 8 0.34 +1Score > 16 indicates identity GEMSTVSK + Oxidation (M)
813 441.2004 1320.5795 1320.6020 -17.0 0 8 0.88 +1Score > 20 indicates identity DGIPLMEEDFR
1117 468.2127 934.4109 934.4178 -7.46 0 8 0.69 +1Score > 20 indicates identity
Score > 19 indicates homology		 MNVGDVQR + Deamidated (NQ); Oxidation (M)
4023 697.8171 2787.2392 2787.2520 -4.59 0 8 0.77 +1Score > 19 indicates identity AGIPLDVMYDVVTNAAGNSWMFENR + 2 Deamidated (NQ); Oxidation (M)
3187 630.2864 1258.5582 1258.5621 -3.06 0 8 0.85 +1Score > 20 indicates identity ISMMPGMPSHR + Oxidation (M)
55 387.1759 772.3372 772.3351 2.67 0 8 0.4 +1Score > 16 indicates identity AQGEDPR + Deamidated (NQ)
555 427.6943 853.3741 853.3674 7.85 0 8 0.35 +1Score > 16 indicates identity VMNMQAK + Deamidated (NQ); 2 Oxidation (M)
1803 522.2372 2084.9199 2084.9287 -4.22 0 8 0.8 +1Score > 19 indicates identity QTSLNGFYQDNGGDADLLR + 2 Deamidated (NQ)
1006 454.7066 907.3986 907.3817 18.6 1 8 0.52 +1Score > 17 indicates identity RDNQAMR + 2 Deamidated (NQ); Oxidation (M)
440 414.1881 1239.5426 1239.5661 -19.0 0 8 0.78 +1Score > 19 indicates identity SPMELMTMLR + 2 Oxidation (M)
3448 657.2986 1312.5827 1312.5686 10.8 1 8 0.83 +1Score > 21 indicates identity
Score > 19 indicates homology		 IMDRMTMNGGR + 2 Oxidation (M)
2619 589.7679 2355.0427 2355.0280 6.24 1 8 0.72 +1Score > 19 indicates identity GEHHGQKGFFAWFNQMFNR + 2 Deamidated (NQ); Oxidation (M)
1499 495.2250 988.4354 988.4461 -10.9 0 8 0.41 +1Score > 20 indicates identity
Score > 16 indicates homology		 VNADPESTR + Deamidated (NQ)
547 427.6943 853.3741 853.3600 16.5 0 8 0.36 +1Score > 16 indicates identity MTSSAGQR + Deamidated (NQ); Oxidation (M)
6805 927.4214 2779.2425 2779.2325 3.60 1 8 0.38 +1Score > 21 indicates identity
Score > 16 indicates homology		 MMSECIYQEGNAFVIMGAGEQLKR + 2 Deamidated (NQ); Oxidation (M)
395 414.1881 826.3617 826.3643 -3.13 0 8 0.8 +1Score > 19 indicates identity MYVNQR + Deamidated (NQ); Oxidation (M)
1815 522.2372 1563.6899 1563.6771 8.17 0 8 0.83 +1Score > 19 indicates identity MMFEFNMAELLR + Deamidated (NQ); 2 Oxidation (M)
4823 765.3478 2293.0215 2292.9845 16.1 0 8 1 +1Score > 21 indicates identity
Score > 20 indicates homology		 LYSSDNMYPDSQQGFAPTVR + 2 Deamidated (NQ); Oxidation (M)
602 427.6943 853.3741 853.3600 16.5 0 8 0.37 +1Score > 16 indicates identity MTSSAGQR + Deamidated (NQ); Oxidation (M)
3419 657.2986 1968.8741 1968.8809 -3.43 1 8 1 +1Score > 21 indicates identity
Score > 20 indicates homology		 MVETSQCVPYPAKADEK + Deamidated (NQ); Oxidation (M)
1010 454.7066 907.3986 907.3891 10.4 0 8 0.55 +1Score > 17 indicates identity DMAMNQAK
718 441.2004 1760.7727 1760.7709 0.99 0 8 0.23 +1Score > 20 indicates identity
Score > 14 indicates homology		 VAAQHSMMPAETESEK + Oxidation (M)
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