MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | test |
| MS data file | : | PRT1346_ENIGMA_14.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 4 Dec 2025 at 15:28:39 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 7,621 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YIBA_ECOLI | 38 | Protein YibA OS=Escherichia coli (strain K12) GN=yibA PE=1 SV=1 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | META_ECOLI | 35 | Homoserine O-succinyltransferase OS=Escherichia coli (strain K12) GN=metA PE=1 SV=4 |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YEAG_ECOLI | 31 | Uncharacterized protein YeaG OS=Escherichia coli (strain K12) GN=yeaG PE=3 SV=1 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SYR_ECOLI | 28 | Arginine--tRNA ligase OS=Escherichia coli (strain K12) GN=argS PE=1 SV=1 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RGP1_ARATH | 24 | UDP-arabinopyranose mutase 1 OS=Arabidopsis thaliana GN=RGP1 PE=1 SV=1 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YCHF_ECOLI | 21 | Ribosome-binding ATPase YchF OS=Escherichia coli (strain K12) GN=ychF PE=1 SV=2 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 6.1 | YCHF_ECOLI |
21 | 39984 | 5 (1) | 1 (1) | 0.08 | |
| Ribosome-binding ATPase YchF OS=Escherichia coli (strain K12) GN=ychF PE=1 SV=2 | |||||||
5 peptide matches (1 non-duplicate, 4 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 3381 |
4 |
643.7925 | 1285.5705 | 1285.5649 | 4.37 | 0 | 21 | 0.032 | 1Score > 18 indicates identity |
U | K.DGDVMNFLFNV.- + Oxidation (M) |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CINAL_ECOLI | 19 | NMN amidohydrolase-like protein YfaY OS=Escherichia coli (strain K12) GN=yfaY PE=1 SV=1 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | FLGF_ECOLI | 15 | Flagellar basal-body rod protein FlgF OS=Escherichia coli (strain K12) GN=flgF PE=3 SV=1 |
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