MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–8 (out of 8)

Page: 1 

+1

Accession Score Description
1 YIBA_ECOLI 38 Protein YibA OS=Escherichia coli (strain K12) GN=yibA PE=1 SV=1

+2

Accession Score Description
1 META_ECOLI 35 Homoserine O-succinyltransferase OS=Escherichia coli (strain K12) GN=metA PE=1 SV=4

+3

Accession Score Description
1 YEAG_ECOLI 31 Uncharacterized protein YeaG OS=Escherichia coli (strain K12) GN=yeaG PE=3 SV=1

+4

Accession Score Description
1 SYR_ECOLI 28 Arginine--tRNA ligase OS=Escherichia coli (strain K12) GN=argS PE=1 SV=1

+5

Accession Score Description
1 RGP1_ARATH 24 UDP-arabinopyranose mutase 1 OS=Arabidopsis thaliana GN=RGP1 PE=1 SV=1

+6

Accession Score Description
1 YCHF_ECOLI 21 Ribosome-binding ATPase YchF OS=Escherichia coli (strain K12) GN=ychF PE=1 SV=2

-7

Accession Score Description
1 CINAL_ECOLI 19 NMN amidohydrolase-like protein YfaY OS=Escherichia coli (strain K12) GN=yfaY PE=1 SV=1
Score Mass Matches Sequences emPAI
7.1 CINAL_ECOLI 19 44483 20 (2) 2 (1) 0.07
NMN amidohydrolase-like protein YfaY OS=Escherichia coli (strain K12) GN=yfaY PE=1 SV=1

-20 peptide matches (5 non-duplicate, 15 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
235 -8 400.6820 1598.6989 1598.6925 4.04 0 4 0.89 +1Score > 16 indicates identity U R.QEVCAMMALNMLR.R + Deamidated (NQ); 2 Oxidation (M)
228   400.6820 1598.6989 1598.6925 4.04 0 (1) 2 +3Score > 16 indicates identity U R.QEVCAMMALNMLR.R + Deamidated (NQ); 2 Oxidation (M)
248   400.6820 1598.6989 1598.6925 4.04 0 (1) 1.9 +2Score > 16 indicates identity U R.QEVCAMMALNMLR.R + Deamidated (NQ); 2 Oxidation (M)
256   400.6820 1598.6989 1598.6925 4.04 0 (2) 1.5 +1Score > 16 indicates identity U R.QEVCAMMALNMLR.R + Deamidated (NQ); 2 Oxidation (M)
263   400.6820 1598.6989 1598.6925 4.04 0 (1) 1.8 +1Score > 16 indicates identity U R.QEVCAMMALNMLR.R + Deamidated (NQ); 2 Oxidation (M)
268   400.6820 1598.6989 1598.6925 4.04 0 (2) 1.4 +3Score > 16 indicates identity U R.QEVCAMMALNMLR.R + Deamidated (NQ); 2 Oxidation (M)
273   400.6820 1598.6989 1598.6925 4.04 0 (1) 1.7 +2Score > 16 indicates identity U R.QEVCAMMALNMLR.R + Deamidated (NQ); 2 Oxidation (M)
274   400.6820 1598.6989 1598.6925 4.04 0 (1) 1.7 +2Score > 16 indicates identity U R.QEVCAMMALNMLR.R + Deamidated (NQ); 2 Oxidation (M)
280   400.6820 1598.6989 1598.6925 4.04 0 (0) 2.2 +3Score > 16 indicates identity U R.QEVCAMMALNMLR.R + Deamidated (NQ); 2 Oxidation (M)
285   400.6820 1598.6989 1598.6925 4.04 0 0 2.2 +1Score > 16 indicates identity U R.QEVCAMMALNMLR.R + Deamidated (NQ); 2 Oxidation (M)
296 +5 400.6820 1598.6989 1598.6925 4.04 0 18 0.033 +1Score > 16 indicates identity U R.QEVCAMMALNMLR.R + Deamidated (NQ); 2 Oxidation (M)
333 +1 400.6820 1598.6989 1598.6925 4.04 0 8 0.39 +1Score > 16 indicates identity U R.QEVCAMMALNMLR.R + Deamidated (NQ); 2 Oxidation (M)
6289 +1 886.9030 1771.7915 1771.7725 10.7 1 7 0.96 +1Score > 19 indicates identity U R.QEVCAMMALNMLRR.W + 2 Deamidated (NQ); 3 Oxidation (M)

+8

Accession Score Description
1 FLGF_ECOLI 15 Flagellar basal-body rod protein FlgF OS=Escherichia coli (strain K12) GN=flgF PE=3 SV=1
Page: 1 

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