MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–8 (out of 8)

Page: 1 

-1

Accession Score Description
1 YIBA_ECOLI 38 Protein YibA OS=Escherichia coli (strain K12) GN=yibA PE=1 SV=1
Score Mass Matches Sequences emPAI
1.1 YIBA_ECOLI 38 32310 25 (3) 1 (1) 0.10
Protein YibA OS=Escherichia coli (strain K12) GN=yibA PE=1 SV=1

-25 peptide matches (1 non-duplicate, 24 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
118 -24 387.1759 772.3372 772.3351 2.65 0 29 0.0028 +1Score > 16 indicates identity U K.DPNGDVR.N + Deamidated (NQ)
21   387.1759 772.3372 772.3351 2.65 0 (8) 0.42 +1Score > 16 indicates identity U K.DPNGDVR.N + Deamidated (NQ)
22   387.1759 772.3372 772.3351 2.65 0 (7) 0.45 +1Score > 16 indicates identity U K.DPNGDVR.N + Deamidated (NQ)
40   387.1759 772.3372 772.3351 2.65 0 (1) 2 +2Score > 16 indicates identity U K.DPNGDVR.N + Deamidated (NQ)
52   387.1759 772.3372 772.3351 2.65 0 (14) 0.1 +1Score > 16 indicates identity U K.DPNGDVR.N + Deamidated (NQ)
59   387.1759 772.3372 772.3351 2.65 0 (2) 1.6 +2Score > 16 indicates identity U K.DPNGDVR.N + Deamidated (NQ)
60   387.1759 772.3372 772.3351 2.65 0 (7) 0.45 +1Score > 16 indicates identity U K.DPNGDVR.N + Deamidated (NQ)
65   387.1759 772.3372 772.3351 2.65 0 (14) 0.097 +1Score > 16 indicates identity U K.DPNGDVR.N + Deamidated (NQ)
67   387.1759 772.3372 772.3351 2.65 0 (12) 0.17 +1Score > 16 indicates identity U K.DPNGDVR.N + Deamidated (NQ)
73   387.1759 772.3372 772.3351 2.65 0 (15) 0.07 +1Score > 16 indicates identity U K.DPNGDVR.N + Deamidated (NQ)
77   387.1759 772.3372 772.3351 2.65 0 (10) 0.26 +1Score > 16 indicates identity U K.DPNGDVR.N + Deamidated (NQ)
80   387.1759 772.3372 772.3351 2.65 0 (4) 0.93 +1Score > 16 indicates identity U K.DPNGDVR.N + Deamidated (NQ)
82   387.1759 772.3372 772.3351 2.65 0 (23) 0.013 +1Score > 16 indicates identity U K.DPNGDVR.N + Deamidated (NQ)
87   387.1759 772.3372 772.3351 2.65 0 (6) 0.57 +1Score > 16 indicates identity U K.DPNGDVR.N + Deamidated (NQ)
120   387.1759 772.3372 772.3351 2.65 0 (7) 0.44 +1Score > 16 indicates identity U K.DPNGDVR.N + Deamidated (NQ)
124   387.1759 772.3372 772.3351 2.65 0 (3) 1.3 +1Score > 16 indicates identity U K.DPNGDVR.N + Deamidated (NQ)
129   387.1759 772.3372 772.3351 2.65 0 (16) 0.062 +1Score > 16 indicates identity U K.DPNGDVR.N + Deamidated (NQ)
136   387.1759 772.3372 772.3351 2.65 0 (2) 1.4 +2Score > 16 indicates identity U K.DPNGDVR.N + Deamidated (NQ)
141   387.1759 772.3372 772.3351 2.65 0 (7) 0.47 +1Score > 16 indicates identity U K.DPNGDVR.N + Deamidated (NQ)
145   387.1759 772.3372 772.3351 2.65 0 (5) 0.84 +1Score > 16 indicates identity U K.DPNGDVR.N + Deamidated (NQ)
153   387.1759 772.3372 772.3351 2.65 0 (18) 0.042 +1Score > 16 indicates identity U K.DPNGDVR.N + Deamidated (NQ)
158   387.1759 772.3372 772.3351 2.65 0 (1) 2.1 +1Score > 16 indicates identity U K.DPNGDVR.N + Deamidated (NQ)
167   387.1759 772.3372 772.3351 2.65 0 (1) 2.1 +2Score > 16 indicates identity U K.DPNGDVR.N + Deamidated (NQ)
168   387.1759 772.3372 772.3351 2.65 0 (13) 0.11 +1Score > 16 indicates identity U K.DPNGDVR.N + Deamidated (NQ)
169   387.1759 772.3372 772.3351 2.65 0 (4) 1 +2Score > 16 indicates identity U K.DPNGDVR.N + Deamidated (NQ)

+2

Accession Score Description
1 META_ECOLI 35 Homoserine O-succinyltransferase OS=Escherichia coli (strain K12) GN=metA PE=1 SV=4

+3

Accession Score Description
1 YEAG_ECOLI 31 Uncharacterized protein YeaG OS=Escherichia coli (strain K12) GN=yeaG PE=3 SV=1

+4

Accession Score Description
1 SYR_ECOLI 28 Arginine--tRNA ligase OS=Escherichia coli (strain K12) GN=argS PE=1 SV=1

+5

Accession Score Description
1 RGP1_ARATH 24 UDP-arabinopyranose mutase 1 OS=Arabidopsis thaliana GN=RGP1 PE=1 SV=1

+6

Accession Score Description
1 YCHF_ECOLI 21 Ribosome-binding ATPase YchF OS=Escherichia coli (strain K12) GN=ychF PE=1 SV=2

+7

Accession Score Description
1 CINAL_ECOLI 19 NMN amidohydrolase-like protein YfaY OS=Escherichia coli (strain K12) GN=yfaY PE=1 SV=1

+8

Accession Score Description
1 FLGF_ECOLI 15 Flagellar basal-body rod protein FlgF OS=Escherichia coli (strain K12) GN=flgF PE=3 SV=1
Page: 1 

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