MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 801–900 (out of 4256)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
530 319.1896 318.1823
531 319.1896 318.1823
532 319.1897 318.1824
533 319.1898 318.1825
534 319.1898 318.1825
535 319.1899 318.1826
536 319.1899 318.1826
537 319.1899 318.1826
538 319.1901 318.1828
539 319.1902 318.1829
540 319.2260 318.2187
541 319.2260 318.2187
542 319.2263 318.2190
543 319.2264 318.2191
544 319.2265 318.2192
545 319.2269 318.2196
546 319.2465 318.2392
547 319.9946 318.9873
548 319.9950 318.9877
549 319.9954 318.9881
550 320.9013 319.8940
551 320.9014 319.8941
552 320.9015 319.8942
553 320.9015 319.8942
554 320.9015 319.8942
555 320.9015 319.8942
556 320.9016 319.8943
557 320.9016 319.8943
558 320.9016 319.8943
559 320.9016 319.8943
560 320.9016 319.8943
561 320.9016 319.8943
562 320.9017 319.8944
563 320.9017 319.8944
564 320.9017 319.8944
565 320.9018 319.8945
566 320.9883 319.9810
567 321.0925 320.0852
568 321.0945 320.0872
569 321.0947 320.0874
570 321.0950 320.0877
571 321.0951 320.0878
572 321.0951 320.0878
573 321.0956 320.0883
574 321.0957 320.0884
575 321.1296 320.1223
576 321.1307 320.1234
577 321.1320 320.1247
578 321.1321 320.1248
579 321.1321 320.1248
580 321.1322 320.1249
581 321.1324 320.1251
582 321.1325 320.1252
583 321.1326 320.1253
584 321.1326 320.1253
585 321.1326 320.1253
586 321.1326 320.1253
587 321.1326 320.1253
588 321.1326 320.1253
589 321.1327 320.1254
590 321.1327 320.1254
591 321.1327 320.1254
592 321.1327 320.1254
593 321.1327 320.1254
594 321.1328 320.1255
595 321.1688 320.1615
596 321.1691 320.1618
597 321.1693 320.1620
598 321.1693 320.1620
599 321.1693 320.1620
600 321.1693 320.1620
601 321.1693 320.1620
602 321.1693 320.1620
603 321.1693 320.1620
604 321.1694 320.1621
605 321.1694 320.1621
606 321.1694 320.1621
607 321.1694 320.1621
608 321.1696 320.1623
609 321.1697 320.1624
610 321.1698 320.1625
611 321.1698 320.1625
612 321.1699 320.1626
613 321.1700 320.1627
614 321.1701 320.1628
615 321.1701 320.1628
616 321.1705 320.1632
617 321.1706 320.1633
618 321.2052 320.1979
619 321.2055 320.1982
620 321.2055 320.1982
621 321.2059 320.1986
622 321.2061 320.1988
623 321.2062 320.1989
624 321.2417 320.2344
625 321.2419 320.2346
626 321.2420 320.2347
627 321.2421 320.2348
628 321.2421 320.2348
629 321.2426 320.2353
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