MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 701–800 (out of 4256)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
430 315.1457 314.1384
431 315.1573 314.1500
432 315.1580 314.1507
433 315.1580 314.1507
434 315.1581 314.1508
435 315.1582 314.1509
436 315.1583 314.1510
437 315.1585 314.1512
438 315.1585 314.1512
439 315.1585 314.1512
440 315.1585 314.1512
441 315.1586 314.1513
442 315.1609 314.1536
443 315.1818 314.1745
444 315.1819 314.1746
445 316.1989 315.1916
446 317.0821 316.0748
447 317.0992 316.0919
448 317.0994 316.0921
449 317.0996 316.0923
450 317.0999 316.0926
451 317.0999 316.0926
452 317.0999 316.0926
453 317.1000 316.0927
454 317.1000 316.0927
455 317.1002 316.0929
456 317.1007 316.0934
457 317.1013 316.0940
458 317.1376 316.1303
459 317.1376 316.1303
460 317.1376 316.1303
461 317.1377 316.1304
462 317.1377 316.1304
463 317.1377 316.1304
464 317.1377 316.1304
465 317.1377 316.1304
466 317.1377 316.1304
467 317.1378 316.1305
468 317.1378 316.1305
469 317.1378 316.1305
470 317.1379 316.1306
471 317.1381 316.1308
472 317.1381 316.1308
473 317.1737 316.1664
474 317.1737 316.1664
475 317.1738 316.1665
476 317.1739 316.1666
477 317.1741 316.1668
478 317.1742 316.1669
479 317.1743 316.1670
480 317.1743 316.1670
481 317.1743 316.1670
482 317.1747 316.1674
483 317.1753 316.1680
484 317.1753 316.1680
485 317.1762 316.1689
486 317.2119 316.2046
487 318.0332 317.0259
488 318.0419 317.0346
489 318.1034 317.0961
490 318.1925 317.1852
491 318.1927 317.1854
492 319.0401 318.0328
493 319.1139 318.1066
494 319.1151 318.1078
495 319.1158 318.1085
496 319.1159 318.1086
497 319.1164 318.1091
498 319.1167 318.1094
499 319.1167 318.1094
500 319.1167 318.1094
501 319.1168 318.1095
502 319.1168 318.1095
503 319.1168 318.1095
504 319.1169 318.1096
505 319.1169 318.1096
506 319.1331 318.1258
507 319.1334 318.1261
508 319.1535 318.1462
509 319.1535 318.1462
510 319.1536 318.1463
511 319.1537 318.1464
512 319.1537 318.1464
513 319.1537 318.1464
514 319.1537 318.1464
515 319.1538 318.1465
516 319.1538 318.1465
517 319.1539 318.1466
518 319.1539 318.1466
519 319.1539 318.1466
520 319.1540 318.1467
521 319.1540 318.1467
522 319.1541 318.1468
523 319.1545 318.1472
524 319.1553 318.1480
525 319.1669 318.1596
526 319.1669 318.1596
527 319.1670 318.1597
528 319.1895 318.1822
529 319.1896 318.1823
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