MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2
MS data file : PRT1311_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:18:13 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,460

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 601–700 (out of 4256)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
330 311.1567 310.1494
331 311.1662 310.1589
332 311.1664 310.1591
333 311.1664 310.1591
334 311.1664 310.1591
335 311.1665 310.1592
336 311.1665 310.1592
337 311.1666 310.1593
338 311.1846 310.1773
339 311.1851 310.1778
340 311.1852 310.1779
341 311.1855 310.1782
342 311.1867 310.1794
343 311.1871 310.1798
344 311.2049 310.1976
345 311.2051 310.1978
346 311.2051 310.1978
347 311.2051 310.1978
348 311.2053 310.1980
349 311.2056 310.1983
350 311.2226 310.2153
351 311.2228 310.2155
352 311.9456 310.9383
353 311.9456 310.9383
354 311.9456 310.9383
355 311.9456 310.9383
356 311.9458 310.9385
357 311.9459 310.9386
358 312.0547 311.0474
359 312.0548 311.0475
360 312.1319 311.1246
361 312.1320 311.1247
362 312.1321 311.1248
363 312.1321 311.1248
364 312.1324 311.1251
365 313.0276 312.0203
366 313.0283 312.0210
367 313.0285 312.0212
368 313.0869 312.0796
369 313.0872 312.0799
370 313.0873 312.0800
371 313.0876 312.0803
372 313.0876 312.0803
373 313.0885 312.0812
374 313.1070 312.0997
375 313.1154 312.1081
376 313.1164 312.1091
377 313.1169 312.1096
378 313.1172 312.1099
379 313.1263 312.1190
380 313.1281 312.1208
381 313.1288 312.1215
382 313.1291 312.1218
383 313.1426 312.1353
384 313.1638 312.1565
385 313.1642 312.1569
386 313.1649 312.1576
387 313.1653 312.1580
388 313.1659 312.1586
389 313.1664 312.1591
390 313.1665 312.1592
391 313.1827 312.1754
392 313.1836 312.1763
393 313.1837 312.1764
394 313.1841 312.1768
395 313.1842 312.1769
396 313.1844 312.1771
397 313.1844 312.1771
398 313.1845 312.1772
399 313.1845 312.1772
400 313.1850 312.1777
401 313.2209 312.2136
402 313.2209 312.2136
403 313.2212 312.2139
404 313.2217 312.2144
405 313.2217 312.2144
406 313.2387 312.2314
407 313.3228 312.3155
408 314.0894 313.0821
409 314.0895 313.0822
410 315.0537 314.0464
411 315.0537 314.0464
412 315.0541 314.0468
413 315.1022 314.0949
414 315.1022 314.0949
415 315.1025 314.0952
416 315.1213 314.1140
417 315.1215 314.1142
418 315.1216 314.1143
419 315.1216 314.1143
420 315.1218 314.1145
421 315.1219 314.1146
422 315.1219 314.1146
423 315.1328 314.1255
424 315.1428 314.1355
425 315.1445 314.1372
426 315.1454 314.1381
427 315.1455 314.1382
428 315.1455 314.1382
429 315.1456 314.1383
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